Phalloidin uptake by the liver of cholestatic rats in vivo, in isolated perfused liver and isolated hepatocytes

1981 ◽  
Vol 316 (3) ◽  
pp. 257-261 ◽  
Author(s):  
A. K. Walli ◽  
E. Wieland ◽  
Th. Wieland
Keyword(s):  
Isolated Hepatocytes ◽  
Perfused Liver ◽  
Isolated Perfused Liver

scholarly journals Effect of thyroid state on cyclic AMP-mediated induction of hepatic phosphoenolpyruvate carboxykinase

1985 ◽  
Vol 226 (1) ◽  
pp. 67-73 ◽  
Author(s):  
W Höppner ◽  
W Süssmuth ◽  
H J Seitz
Keyword(s):  
Cyclic Amp ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Close Correlation ◽  
Perfused Liver ◽  
Thyroid State ◽  
Fed State ◽  
Isolated Perfused Liver ◽  
Translatable Mrna

Hepatic phosphoenolpyruvate carboxykinase (PEPCK) is significantly increased in the hyperthyroid starved rat, and moderately decreased in the hypothyroid starved rat. As tri-iodothyronine by itself has only a small and sustained effect on the induction of this enzyme, as was previously shown in the isolated perfused organ, the effect of hypo- and hyper-thyroidism on the increase in cytosolic PEPCK provoked by dibutyryl cyclic AMP (Bt2cAMP) was investigated in vivo and in the isolated perfused liver. Compared with euthyroid fed controls, in hypothyroid fed rats Bt2cAMP provoked in 2 h only a small increase in translatable mRNA coding for PEPCK. In contrast, in hyperthyroid animals PEPCK mRNA as measured by translation in vitro was already increased in the fed state, and further enhanced by Bt2cAMP injection to values as in euthyroid controls. Under all thyroid states a close correlation between PEPCK mRNA activity and PEPCK synthesis was observed. In the isolated perfused liver from the hyperthyroid fed rat, the increase in PEPCK provoked by Bt2cAMP or Bt2cAMP + isobutylmethylxanthine was considerably enhanced compared with those obtained in livers of hypothyroid rats. Also, adrenaline provoked a stimulated induction of PEPCK in hyperthyroid rats compared with hypothyroid rats. To summarize, our data indicate that the primary action of thyroid hormones on the synthesis of hepatic cytosolic PEPCK is to accelerate the cyclic AMP- or adrenaline-induction of the enzyme, acting primarily at a pretranslational level.

Glutathione Conjugation of 4-Hydroxy-trans-2,3-nonenal in the Rat in Vivo, the Isolated Perfused Liver and Erythrocytes

1999 ◽  
Vol 159 (3) ◽  
pp. 214-223 ◽  
Author(s):  
Piet J.M. Boon ◽  
H.Susana Marinho ◽  
Roelof Oosting ◽  
Gerard J. Mulder
Keyword(s):  
Perfused Liver ◽  
Glutathione Conjugation ◽  
Isolated Perfused Liver

A comparative study of drug metabolism in the isolated perfused liver and in vivo in rats

1970 ◽  
Vol 9 (1) ◽  
pp. 99-105 ◽  
Author(s):  
C. Von Bahr ◽  
B. Alexanderson ◽  
D.L. Azarnoff ◽  
F. Sjöqvist ◽  
S. Orrenius
Keyword(s):  
Drug Metabolism ◽  
Comparative Study ◽  
Perfused Liver ◽  
Isolated Perfused Liver

scholarly journals Mitochondrial and cytosolic ATP/ADP ratios in rat liver in vivo

1981 ◽  
Vol 200 (2) ◽  
pp. 405-408 ◽  
Author(s):  
W D Schwenke ◽  
S Soboll ◽  
H J Seitz ◽  
H Sies
Keyword(s):  
Rat Liver ◽  
Oxygen Supply ◽  
Isolated Hepatocytes ◽  
Perfused Liver ◽  
Mitochondrial Matrix ◽  
Atp Content ◽  
Anaesthetized Rats

The ratio of ATP content/ADP content in livers from unanaesthetized fed rat was 0.9 in the mitochondrial matrix and 6.9 in the cytosol; the values for starved (48 h) animals were 1.0 and 5.9 respectively. The mitochondrial ratios observed in unanaesthetized animals were higher than in haemoglobin-free-perfused liver and lower than in isolated hepatocytes. Possible reasons for these differences may be related to oxygen supply and/or other factors. Further, data from anaesthetized rats with the liver exposed are given: mitochondrial ATP/ADP ratios were decreased with pentobarbital, but less so with ketamine as narcotic agent.

Hepatic metabolism of meal-fed rats: Studies in vivo and in the isolated perfused liver

1990 ◽  
Vol 48 (2) ◽  
pp. 247-253 ◽  
Author(s):  
R.B. Bazotte ◽  
J. Constantin ◽  
N.S. Hell ◽  
A. Bracht
Keyword(s):  
Hepatic Metabolism ◽  
Perfused Liver ◽  
Isolated Perfused Liver

Influence of hepatic taurine concentration on bile acid conjugation with taurine.

1977 ◽  
Vol 232 (1) ◽  
pp. E75 ◽  
Author(s):  
W G Hardison ◽  
J H Proffitt
Keyword(s):  
Bile Acid ◽  
Cholic Acid ◽  
Sodium Cholate ◽  
Total Bile Acid ◽  
Perfused Liver ◽  
Taurine Concentration ◽  
Isolated Perfused Liver ◽  
Liver Homogenates

In vitro, addition of taurine to liver homogenates increases the proportion of cholic acid conjugated with taurine. In the present study, the relation between hepatic taurine concentration and the proportion of infused sodium cholate conjugated with taurine was studied in the whole organ. The isolated perfused liver was studied to eliminate possible transfer of taurine to or from the large extrahepatic poosl present in vivo. During cholate infusion, the proportion of taurocholate excreted in bile decreased, and the proportion of glycocholate increased in a complementary fashion. Infusion of taurine with cholate prevented these changes. Hepatic taurine concentration, calculated from measured hepatic taurine concentrations before and at the end of cholate infusion, fell. Fall in proportion of total bile acid excreted as taurocholate was most rapid at low hepatic taurine concentrations between about 1.4 and 0.65 mumol/g liver. Hepatic taurine concentrations is a major determinant of the proportion of bile acid conjugated with taurine.

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