Die kinetische Bestimmung der alkalischen Serum-Phosphatase

1968 ◽  
Vol 234 (6) ◽  
pp. 459-459
Author(s):  
P. Gerhards
Keyword(s):  
Serum Phosphatase

scholarly journals STUDIES ON SERUM PHOSPHATASE ACTIVITY

1938 ◽  
Vol 124 (3) ◽  
pp. 631-637
Author(s):  
S.J. Thannhauser ◽  
Max Reichel ◽  
Jerome F. Grattan ◽  
Stephen J. Maddock
Keyword(s):  
Phosphatase Activity ◽  
Serum Phosphatase

SERUM PHOSPHATASE IN CASES OF BONE TUMOUR

The Lancet ◽  
1940 ◽  
Vol 235 (6094) ◽  
pp. 1074-1075 ◽  
Author(s):  
Stanford Cade ◽  
N.F. Maclagan ◽  
R.F. Townsend
Keyword(s):  
Bone Tumour ◽  
Serum Phosphatase

Quantification of Serum Inorganic Phosphorus, Phosphatase, and Urinary Phosphate without Preliminary Treatment

1970 ◽  
Vol 16 (9) ◽  
pp. 776-780 ◽  
Author(s):  
Jesse F Goodwin
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Direct Method ◽  
Reference Method ◽  
Inorganic Phosphorus ◽  
Preliminary Treatment ◽  
Reduction Procedure ◽  
Phosphorus Forms ◽  
A Direct Method ◽  
Serum Phosphatase

Abstract A direct method has been devised for determining serum and urinary inorganic phosphorus and serum phosphatase activity. Inorganic phosphorus forms a phosphomolybdate complex in the presence of borate. The complex is reduced with ascorbic acid. The resulting suspension is solubilized with carbonate and the absorbance measured at 720 nm. Samples containing low to moderate concentrations of bilirubin may be assayed by the method, and it compares favorably with a p-semidine reduction procedure with use of a TCA filtrate. Alkaline phosphatase values obtained by the method in which a β-glycerophosphate substrate is used compare favorably with a reference method in which p-nitrophenolphosphate is used as a substrate. Citrates, fluorides, nitrites, oxalates, and mannitol do not appreciably affect results obtained by the method.

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