Ribonucleotides during the regeneration of the rat kidney after lesions by folic acid, HgCl2 and ligation of the ureter

1974 ◽  
Vol 284 (4) ◽  
pp. 383-393 ◽  
Author(s):  
J. Oertel ◽  
H. Herken
Keyword(s):  
Folic Acid ◽  
Rat Kidney ◽  
Ligation Of The Ureter

The Morphology of the Rat Kidney Following Folic Acid Administration

1970 ◽  
Vol 28 ◽  
pp. 200-201
Author(s):  
Aline Byrnes ◽  
Elsa E. Ramos ◽  
Minoru Suzuki ◽  
E.D. Mayfield
Keyword(s):  
Folic Acid ◽  
De Novo ◽  
Specific Activity ◽  
Rat Kidney ◽  
Present Report ◽  
Intracellular Localization ◽  
Male Rats ◽  
Renal Hypertrophy ◽  
Electron Microscopic ◽  
Biochemical Alterations

Renal hypertrophy was induced in 100 g male rats by the injection of 250 mg folic acid (FA) dissolved in 0.3 M NaHCO3/kg body weight (i.v.). Preliminary studies of the biochemical alterations in ribonucleic acid (RNA) metabolism of the renal tissue have been reported recently (1). They are: RNA content and concentration, orotic acid-c14 incorporation into RNA and acid soluble nucleotide pool, intracellular localization of the newly synthesized RNA, and the specific activity of enzymes of the de novo pyrimidine biosynthesis pathway. The present report describes the light and electron microscopic observations in these animals. For light microscopy, kidney slices were fixed in formalin, embedded, sectioned, and stained with H & E and PAS.

scholarly journals Effects of folic acid on rat kidney exposed to 900 MHz electromagnetic radiation

2017 ◽  
Vol 5 (4) ◽  
pp. 198 ◽  
Author(s):  
ÖmürGülsüm Deniz ◽  
ElfideGizem Kıvrak ◽  
ArifeAhsen Kaplan ◽  
BerrinZuhal Altunkaynak
Keyword(s):  
Folic Acid ◽  
Electromagnetic Radiation ◽  
Rat Kidney

Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy

2004 ◽  
Vol 287 (2) ◽  
pp. C517-C526 ◽  
Author(s):  
Ruben M. Sandoval ◽  
Michael D. Kennedy ◽  
Philip S. Low ◽  
Bruce A. Molitoris
Keyword(s):  
Folic Acid ◽  
Proximal Tubule ◽  
Rat Kidney ◽  
Proximal Tubules ◽  
Apical Surface ◽  
Proximal Tubule Cells ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Tubule Cells ◽  
Folate Conjugate

Intravital two-photon microscopy was used to follow the uptake and trafficking of fluorescent conjugates of folic acid in the rat kidney. Intravenously administered folate-linked dye molecules quickly filled the plasma volume but not cellular components of the blood. Glomerular filtration occurred immediately and binding to proximal tubule cells was seen within seconds. Fluorescence from a pH-insensitive conjugate of folic acid, folate Texas red (FTR), was readily observed on the apical surface of the proximal tubules and in multiple cellular compartments, but little binding or uptake could be detected in any other kidney cells. Fluorescence from a pH-sensitive conjugate of folic acid, folate fluorescein, was seen only on the apical surface of proximal tubule cells, suggesting that internalized folate conjugates are localized to acidic compartments. The majority of the FTR conjugate internalized by proximal tubules accumulated within a lysosomal pool, as determined by colocalization studies. However, portions of FTR were also shown by electron microscopy to undergo transcytosis from apical to basal domains. Additional studies with colchicine, which is known to depolymerize microtubules and interrupt transcytosis, produced a marked reduction in endocytosis of FTR, with accumulation limited to the subapical region of the cell. No evidence of cytosolic release of either folate conjugate was observed, which may represent a key difference from the cytosolic deposition seen in neoplastic cells. Together, these data support the argument that folate conjugates (and, by extrapolation, physiological folate) bind to the apical surface of proximal tubule cells and are transported into and across the cells in endocytic compartments.

Epidermal growth factor receptor regulation in rat kidney: two models of renal growth

1989 ◽  
Vol 257 (6) ◽  
pp. F1059-F1064 ◽  
Author(s):  
M. T. Behrens ◽  
A. L. Corbin ◽  
M. K. Hise
Keyword(s):  
Folic Acid ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Proximal Tubule ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Proximal Tubule Cell ◽  
Tubule Cell ◽  
Rat Kidney Cortex ◽  
Epidermal Growth

The binding of 125I-labeled epidermal growth factor (EGF) to plasma membranes prepared from rat kidney cortex was studied following unilateral nephrectomy, a model of proximal tubule cell hypertrophy, and following the administration of folic acid, a model of proximal tubule cell hyperplasia. Binding of 125I-EGF was a linear function of basolateral membrane protein content and time of incubation. Specific binding to luminal brush-border membranes was not evident in these studies. Neither insulin nor insulin-like growth factor I could displace EGF binding, indicating that binding was specific. Scatchard analysis revealed a single binding site. The KD in sham-operated animals 48 h after surgery was 11.2 +/- 1.4 nM, whereas Bmax averaged 95.2 +/- 4.1 fmol/mg protein (n = 3). Similar values were obtained in nephrectomized animals. The Bmax of folic acid-untreated animals averaged 212.5 +/- 6.9 fmol/mg 48 h after administration, whereas that of vehicle-injected controls averaged 85.4 +/- 9.2 (n = 3, P less than 0.001). Differences in binding were not related to changes in affinity, ligand degradation by the preparations, or receptor binding of endogenous EGF. These data indicate that regeneration following folic acid administration is associated with an upregulation of proximal nephron EGF receptors that may play an important role in the mitogenic response.

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