Failure of denitrifying bacteria to utilize benzoic acid under anaerobic conditions with nitrate as the only terminal electron acceptor

1980 ◽  
Vol 9 (2) ◽  
pp. 133-135 ◽  
Author(s):  
W. Fabig ◽  
J. C. G. Ottow ◽  
F. M�ller
Keyword(s):  
Benzoic Acid ◽  
Electron Acceptor ◽  
Denitrifying Bacteria ◽  
Anaerobic Conditions ◽  
Terminal Electron Acceptor

scholarly journals The fixA and fixB Genes Are Necessary for Anaerobic Carnitine Reduction in Escherichia coli

2002 ◽  
Vol 184 (14) ◽  
pp. 4044-4047 ◽  
Author(s):  
Angelique Walt ◽  
Michael L. Kahn
Keyword(s):  
Escherichia Coli ◽  
Electron Acceptor ◽  
Anaerobic Conditions ◽  
Terminal Electron Acceptor ◽  
E Coli ◽  
Carnitine Metabolism

ABSTRACT In Escherichia coli, the use of carnitine as a terminal electron acceptor depends on a functional caiTABCDE operon. It had been suggested that the adjacent but divergent fixABCX operon is also required for carnitine metabolism, perhaps to provide electrons for carnitine reduction. We have constructed E. coli fixA and fixB mutants and find that they are unable to reduce carnitine to γ-butyrobetaine under anaerobic conditions.

scholarly journals Isolation and Characterization of Diverse Halobenzoate-Degrading Denitrifying Bacteria from Soils and Sediments

2000 ◽  
Vol 66 (8) ◽  
pp. 3446-3453 ◽  
Author(s):  
Bongkeun Song ◽  
Norberto J. Palleroni ◽  
Max M. Häggblom
Keyword(s):  
Electron Acceptor ◽  
Cellular Fatty Acid ◽  
Denitrifying Bacteria ◽  
Rrna Gene ◽  
Terminal Electron Acceptor ◽  
Ecological Sites ◽  
Isolation And Characterization ◽  
Gene Sequence Analysis ◽  
Soils And Sediments

ABSTRACT Denitrifying bacteria capable of degrading halobenzoates were isolated from various geographical and ecological sites. The strains were isolated after initial enrichment on one of the monofluoro-, monochloro-, or monobromo-benzoate isomers with nitrate as an electron acceptor, yielding a total of 33 strains isolated from the different halobenzoate-utilizing enrichment cultures. Each isolate could grow on the selected halobenzoate with nitrate as the terminal electron acceptor. The isolates obtained on 2-fluorobenzoate could use 2-fluorobenzoate under both aerobic and denitrifying conditions, but did not degrade other halobenzoates. In contrast, the 4-fluorobenzoate isolates degraded 4-fluorobenzoate under denitrifying conditions only, but utilized 2-fluorobenzoate under both aerobic and denitrifying conditions. The strains isolated on either 3-chlorobenzoate or 3-bromobenzoate could use 3-chlorobenzoate, 3-bromobenzoate, and 2- and 4-fluorobenzoates under denitrifying conditions. The isolates were identified and classified on the basis of 16S rRNA gene sequence analysis and their cellular fatty acid profiles. They were placed in nine genera belonging to either the α-, β-, or γ-branch of theProteobacteria, namely, Acidovorax,Azoarcus, Bradyrhizobium,Ochrobactrum, Paracoccus,Pseudomonas, Mesorhizobium,Ensifer, and Thauera. These results indicate that the ability to utilize different halobenzoates under denitrifying conditions is ubiquitously distributed in theProteobacteria and that these bacteria are widely distributed in soils and sediments.

scholarly journals Investigation of suitable conditions for the growth and producing biofilm of denitrifying bacteria

2016 ◽  
Vol 14 (1) ◽  
pp. 191-196
Author(s):  
Hoàng Phương Hà ◽  
Nguyễn Quang Huy ◽  
Hoàng Thị Yến
Keyword(s):  
Aerobic Condition ◽  
Denitrifying Bacteria ◽  
Nitrifying Bacteria ◽  
Contaminated Water ◽  
Rrna Gene ◽  
Anaerobic Conditions ◽  
16S Rrna Gene Analysis ◽  
Lower Efficiency ◽  
Terminal Electron Acceptor ◽  
Selection Of

Denitrification occurs under anaerobic conditions, utilizing organic substances such as a carbon source and nitrate as the terminal electron acceptor. Some bacteria strains that are capable of producing biofilm and are attached to the carrier for the applications of the nitrate contaminated water treatment. This process is done simultaneously with the process of nitrification, nitrifying bacteria on the biofilm surface (ocic zone), denitrifying bacteria under biofilm (anoxic zone). The selection of suitable conditions for the growth and production of biofilm of denitrifying bacteria is the target of this research. Two strains D10 and D32 are capable to reduce nitrate and created the best biofilm. Optimal conditions for the strain growth were: 37oC, pH 6.6 - 7. Supplementation of 0.006% M1, E2 (methanol, ethanol) increased the reducing N-NO3 to 80% and 100% respectively. Denitrification process still occured in aerobic condition but with lower efficiency (25.8%) byD32 strain. Based on 16S rRNA gene analysis, D10 strain was the closest relative with Bacillus fusiformis, D32 closest to Pseudomonas denitrifricans.

Sulfate as a terminal electron acceptor in toluene oxidation in anaerobic conditions

2020 ◽  
Vol 22 (4) ◽  
pp. 157-167
Author(s):  
I. I. Ivanenkо ◽  
E. Y. Lapatina
Keyword(s):  
Electron Acceptor ◽  
Waste Water Treatment ◽  
Process Intensification ◽  
Petroleum Products ◽  
Electron Acceptors ◽  
Laboratory Research ◽  
Anaerobic Conditions ◽  
Active Sludge ◽  
Toluene Oxidation ◽  
Terminal Electron Acceptor

In aerobic conditions, many of microorganisms cause decomposition of saturated hydrocarbons. Little publications are available relative to anaerobic transformation of these compounds, which provides substantial сapiltal saving for waste water treatment. At the same time, cultures found among aerobic decomposers of petroleum products are characterized by the ability to use elements with variable valence as terminal electron acceptors in oxidation of organic substances. Their ability to decompose aliphatic hydrocarbons helps to identify some of them.Purpose: studying the ability of a selected association of immobilized bacteria on a fibrous carrier to utilize sulfate as a terminal electron acceptor for toluene oxidation.Methodology/approach: Analytical summarizing of results, literature review, laboratory research based on standard and modern up-to-date methodologies with the use of modern analytical equipment.Findings: The availability of using microorganism selection is shown for expanding the range of polluting strippants in biological purification; the main directions are determined for the process intensification by immobilization of active sludge on a fibrous carrier. The ability of microorganisms to oxidize toluene under oxygen-free (anaerobic) conditions is studied in the laboratory conditions.Research implications: monoaromatic hydrocarbons, toluene, in particular, can be changed by selected associations of decomposers using terminal electron acceptors in oxidation of nitrates and sulfates.

Function of the Rhizobium etli CFN42 nirK gene in nitrite metabolism

2005 ◽  
Vol 33 (1) ◽  
pp. 162-163 ◽  
Author(s):  
E. Bueno ◽  
N. Gómez-Hernández ◽  
L. Girard ◽  
E.J. Bedmar ◽  
M.J. Delgado
Keyword(s):  
Electron Acceptor ◽  
Mutational Analysis ◽  
Deficient Mutant ◽  
Rhizobium Etli ◽  
Wild Type ◽  
Anaerobic Conditions ◽  
Gene Encoding ◽  
Terminal Electron Acceptor ◽  
Nirk Gene ◽  
Nitrite Uptake

Rhizobium etli CFN42 is not capable of growing anaerobically with nitrate but it grows with nitrite as a terminal electron acceptor. This bacterium contains the nirK gene encoding the copper-containing Nir (nitrite reductase), which is located on the cryptic plasmid pCFN42f. Mutational analysis has demonstrated that a nirK deficient mutant was not capable of growing under nitrite-respiring conditions. Moreover, microaerobic growth of this mutant was inhibited by the presence of nitrite. Nir activity and nitrite uptake were highly diminished in a nirK mutant, compared with the wild-type levels after incubation under anaerobic conditions. Our results suggest that the copper-containing Nir may have both a respiratory and a nitrite-detoxifying role in R. etli.

Effects of terminal electron acceptor strength on film morphology and ternary memory performance of triphenylamine donor based devices

2013 ◽  
Vol 1 (24) ◽  
pp. 3816 ◽  
Author(s):  
Hao Zhuang ◽  
Qijian Zhang ◽  
Yongxiang Zhu ◽  
Xufeng Xu ◽  
Haifeng Liu ◽  
...  
Keyword(s):  
Electron Acceptor ◽  
Memory Performance ◽  
Film Morphology ◽  
Terminal Electron Acceptor

Magnetovibrio blakemorei gen. nov., sp. nov., a magnetotactic bacterium ( Alphaproteobacteria : Rhodospirillaceae ) isolated from a salt marsh

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1824-1833 ◽  
Author(s):  
Dennis A. Bazylinski ◽  
Timothy J. Williams ◽  
Christopher T. Lefèvre ◽  
Denis Trubitsyn ◽  
Jiasong Fang ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Electron Acceptor ◽  
Anaerobic Growth ◽  
Rrna Gene ◽  
Magnetotactic Bacterium ◽  
Single Chain ◽  
Circular Chromosome ◽  
Terminal Electron Acceptor ◽  
Content Type ◽  
Link Type

A magnetotactic bacterium, designated strain MV-1T, was isolated from sulfide-rich sediments in a salt marsh near Boston, MA, USA. Cells of strain MV-1T were Gram-negative, and vibrioid to helicoid in morphology. Cells were motile by means of a single polar flagellum. The cells appeared to display a transitional state between axial and polar magnetotaxis: cells swam in both directions, but generally had longer excursions in one direction than the other. Cells possessed a single chain of magnetosomes containing truncated hexaoctahedral crystals of magnetite, positioned along the long axis of the cell. Strain MV-1T was a microaerophile that was also capable of anaerobic growth on some nitrogen oxides. Salinities greater than 10 % seawater were required for growth. Strain MV-1T exhibited chemolithoautotrophic growth on thiosulfate and sulfide with oxygen as the terminal electron acceptor (microaerobic growth) and on thiosulfate using nitrous oxide (N2O) as the terminal electron acceptor (anaerobic growth). Chemo-organoautotrophic and methylotrophic growth was supported by formate under microaerobic conditions. Autotrophic growth occurred via the Calvin–Benson–Bassham cycle. Chemo-organoheterotrophic growth was supported by various organic acids and amino acids, under microaerobic and anaerobic conditions. Optimal growth occurred at pH 7.0 and 26–28 °C. The genome of strain MV-1T consisted of a single, circular chromosome, about 3.7 Mb in size, with a G+C content of 52.9–53.5 mol%.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MV-1T belongs to the family Rhodospirillaceae within the Alphaproteobacteria , but is not closely related to the genus Magnetospirillum . The name Magnetovibrio blakemorei gen. nov., sp. nov. is proposed for strain MV-1T. The type strain of Magnetovibrio blakemorei is MV-1T ( = ATCC BAA-1436T  = DSM 18854T).

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