Immunocytochemical localization of hepatic fatty acid binding protein in the liver of fed and fasted rats

1988 ◽  
Vol 89 (4) ◽  
pp. 317-322 ◽  
Author(s):  
S. Iseki ◽  
H. Kondo ◽  
M. Hitomi ◽  
T. Ono
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Immunocytochemical Localization ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Hepatic Fatty Acid ◽  
Fasted Rats

scholarly journals Studies on fatty acid-binding proteins. Changes in the concentration of hepatic fatty acid-binding protein during development in the rat

1987 ◽  
Vol 242 (3) ◽  
pp. 919-922 ◽  
Author(s):  
M Sheridan ◽  
T C I Wilkinson ◽  
D C Wilton
Keyword(s):  
Fatty Acid ◽  
Binding Proteins ◽  
Fatty Acid Binding Protein ◽  
Fluorescence Enhancement ◽  
Binding Protein ◽  
Fold Increase ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Hepatic Fatty Acid

The concentration of hepatic fatty acid-binding protein was determined in the livers of rats at various stages of development from foetus to young adult. Fatty acid-binding protein concentrations were determined by quantifying the fluorescence enhancement on the binding of the fluorescent probe 11-(dansylamino)-undecanoic acid. A 20-fold increase in the concentration of the protein was observed between the foetus and adult, and this increase was confirmed by immuno-blotting. No other protein in the 14,000-Mr range was observed in the foetus. Possible alternative fatty acid-binding proteins could not be detected in h.p.l.c.-fractionated foetal cytosol by the fluorescence-enhancement method.

scholarly journals Light microscopic localization of hepatic fatty acid binding protein mRNA in jejunal epithelia of rats using in situ hybridization, immunohistochemical, and autoradiographic techniques.

1990 ◽  
Vol 38 (1) ◽  
pp. 111-115 ◽  
Author(s):  
S Iseki ◽  
H Kondo
Keyword(s):  
In Situ Hybridization ◽  
Fatty Acid ◽  
Epithelial Cells ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Hepatic Fatty Acid ◽  
Fabp Gene

An in situ hybridization technique using a [35S]-labeled oligonucleotide probe was employed, in combination with immunohistochemistry and autoradiography, to examine gene expression for hepatic fatty acid binding protein (FABP) in the jejunal epithelia from both fed and fasted rats. In rats fed ad libitum, immunoreactivity and mRNA signal for FABP were localized to the absorptive epithelial cells lining the villus, whereas they were absent in the crypt epithelial cells. The level of FABP mRNA was relatively low in the tip of the villus, although FABP immunoreactivity remained high in this area. Animals fasted for 3 days exhibited a downward shift of the lower boundary of the FABP-expressing cell population into the middle portion of the crypt, in terms of the immunoreactivity and the mRNA signal. The proliferative cell compartment of the crypt, as revealed by [3H]-TdR incorporation, showed no substantial change in size between the fed and fasted states. The present results provided evidence that (a) during the differentiation and upward migration of the absorptive epithelial cells, the expression of FABP gene begins at the crypt-villus junction and declines before the cells reach the villus tip, and (b) fasting induces an earlier expression of the FABP gene in the maturing crypt epithelial cells.

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