Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity

1984 ◽  
Vol 22 (1-2) ◽  
pp. 115-124 ◽  
Author(s):  
Douglas K. Rex ◽  
William F. Bosron ◽  
Ting-Kai Li
Keyword(s):  
Enzyme Activity ◽  
Alcohol Dehydrogenase ◽  
Liver Enzyme ◽  
Inbred Strains ◽  
Purification And Characterization ◽  
Liver Enzyme Activity ◽  
Total Liver

scholarly journals Partial purification and characterization of an endo-α-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242

1992 ◽  
Vol 288 (2) ◽  
pp. 475-482 ◽  
Author(s):  
I Ishii-Karakasa ◽  
H Iwase ◽  
K Hotta ◽  
Y Tanaka ◽  
S Omura
Keyword(s):  
Enzyme Activity ◽  
Culture Medium ◽  
Gel Filtration ◽  
Partial Purification ◽  
Gastric Mucus ◽  
Purification And Characterization ◽  
Streptomyces Sp ◽  
Optimum Ph ◽  
New Type

For the purification of a new type of endo-alpha-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242 (endo-GalNAc-ase-S) [Iwase, Ishii, Ishihara, Tanaka, Omura & Hotta (1988) Biochem. Biophys. Res. Commun. 151, 422-428], a method for assaying enzyme activity was established. Using purified pig gastric mucus glycoprotein (PGM) as the substrate, oligosaccharides liberated from PGM were pyridylaminated, and the reducing terminal sugars of oligosaccharides larger than Gal beta 1-3GalNAc were analysed by h.p.1.c. The crude enzyme of endo-GalNAc-ase-S was prepared as an 80% (w/v) ammonium sulphate precipitate from the concentrated culture medium. The enzyme was partially purified by gel chromatofocusing and subsequent DEAE-Toyopearl chromatography. Endo-enzyme activity eluted around pI 4.8 on a gel chromatofocusing column and eluted with 0.19-0.25 M-NaCl on a DEAE-Toyopearl column. In the enzyme fraction obtained, no exo-glycosidases or proteases could be detected. The molecular mass of the enzyme was estimated as 105 kDa by gel filtration, and the optimum pH was 5.5. Endo-GalNAc-ase-S hydrolysed the O-glycosidic linkage between GalNAc and Ser (Thr) in 3H-labelled and unlabelled asialofetuin, liberating both the disaccharide (Gal beta 1-3GalNAc) and the tetrasaccharide [Gal beta 1-3 (Gal beta 1-4GlcNAc beta 1-6)GalNAc]. When endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. (endo-GalNac-ase-A) was incubated with 3H-labelled and unlabelled asialofetuin, only the disaccharide (Gal beta 1-3GalNAc) was liberated.

scholarly journals The effect of paracetamol on human Chang liver cells infected by different dengue serotypes based on liver enzyme activity

2012 ◽  
Vol 16 ◽  
pp. e107
Author(s):  
H. Roslee ◽  
C. Hui Yee ◽  
S. Fakurazi ◽  
F. Azizi Jalilian ◽  
F. Othman ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Liver Enzyme ◽  
Liver Cells ◽  
Liver Enzyme Activity ◽  
Chang Liver

Regulation of Corticosterone Levels and Liver Enzyme Activity in Aging Rats

1975 ◽  
pp. 209-228 ◽  
Author(s):  
Gary W. Britton ◽  
Samuel Rotenberg ◽  
Colette Freeman ◽  
Venera J. Britton ◽  
Karen Karoly ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Liver Enzyme ◽  
Aging Rats ◽  
Liver Enzyme Activity
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