EcoRI restriction-site polymorphism of the albumin gene in different inbred strains of rat

1982 ◽  
Vol 20 (11-12) ◽  
pp. 1105-1115 ◽  
Author(s):  
G�rard Lucotte ◽  
Andras Gal ◽  
Jean-Louis Nahon ◽  
Jos� M. Sala-Trepat
Keyword(s):  
Restriction Site ◽  
Inbred Strains ◽  
Albumin Gene ◽  
Restriction Site Polymorphism ◽  
Ecori Restriction

Structural basis for restriction-site polymorphism at the albumin locus in inbred strains of rats

1985 ◽  
Vol 23 (3-4) ◽  
pp. 257-266 ◽  
Author(s):  
Andras Gal ◽  
Jean-Louis Nahon ◽  
Gerard Lucotte ◽  
Tamas Erdos ◽  
Jos� M. Sala-Trepat
Keyword(s):  
Restriction Site ◽  
Inbred Strains ◽  
Structural Basis ◽  
Restriction Site Polymorphism

scholarly journals A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site

2008 ◽  
Vol 10 (5) ◽  
pp. 469-474 ◽  
Author(s):  
Shujian Liang ◽  
Harold N. Bass ◽  
Hanlin Gao ◽  
Caroline Astbury ◽  
Mehdi R. Jamehdor ◽  
...  
Keyword(s):  
Restriction Site ◽  
Fragile X ◽  
Base Change ◽  
Full Mutation ◽  
Ecori Restriction

Detection of a restriction site polymorphism within the human ?-globin gene complex

1985 ◽  
Vol 69 (2) ◽  
pp. 144-146 ◽  
Author(s):  
G. Assum ◽  
E. -U. Griese ◽  
J. Horst
Keyword(s):  
Restriction Site ◽  
Globin Gene ◽  
Gene Complex ◽  
Restriction Site Polymorphism

Identification of an EcoRI restriction site for a rapid and precise determination of β-asarone-free Acorus calamus cytotypes

2005 ◽  
Vol 66 (5) ◽  
pp. 507-514 ◽  
Author(s):  
Cinzia M. Bertea ◽  
Chiara M.M. Azzolin ◽  
Simone Bossi ◽  
Giovanni Doglia ◽  
Massimo E. Maffei
Keyword(s):  
Restriction Site ◽  
Precise Determination ◽  
Acorus Calamus ◽  
Ecori Restriction

scholarly journals Chromosomal mapping of murine c-fes and c-src genes.

1984 ◽  
Vol 4 (5) ◽  
pp. 978-981 ◽  
Author(s):  
C Blatt ◽  
M E Harper ◽  
G Franchini ◽  
M N Nesbitt ◽  
M I Simon
Keyword(s):  
Kinase Activity ◽  
Restriction Site ◽  
Mouse Genome ◽  
Inbred Strains ◽  
Chromosomal Mapping ◽  
Chromosome 2 ◽  
Distal Half ◽  
Viral Oncogenes ◽  
Tyrosine Specific Kinase ◽  
Restriction Site Polymorphisms

The murine homologs of two viral oncogenes associated with tyrosine-specific kinase activity have been assigned to different loci in the mouse genome. The segregation of restriction site polymorphisms, as detected by probes that are specific for endogenous c-fes and c-src sequences, was followed in the DNA of recombinant inbred strains. The c-fes gene was mapped to the proximal portion of chromosome 7, very close to the Gpi-1 locus, whereas c-src was linked to the Psp locus on the distal half of chromosome 2.

scholarly journals Restriction Site Polymorphism in the Ribosomal DNA of Eight Species of Canidae and Mustelidae.

CYTOLOGIA ◽  
1993 ◽  
Vol 58 (2) ◽  
pp. 223-230 ◽  
Author(s):  
Tetsuji Hosoda ◽  
Hitoshi Suzuki ◽  
Takuzo Yamada ◽  
Kimiyuki Tsuchiya
Keyword(s):  
Ribosomal Dna ◽  
Restriction Site ◽  
Restriction Site Polymorphism

scholarly journals Modification of recombinant human epidermal growth factor (rh-EGF) expression vector by site-directed mutagenesis for therapeutic protein production

2019 ◽  
Vol 24 (1) ◽  
pp. 8
Author(s):  
Achmad Rodiansyah ◽  
Riyona Desvy Pratiwi ◽  
Sabighoh Zanjabila ◽  
Asrul Muhamad Fuad
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Expression Vector ◽  
Restriction Site ◽  
Stop Codon ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Human Epidermal Growth Factor ◽  
Epidermal Growth ◽  
Ecori Restriction

Recombinant human epidermal growth factor (rh-EGF) has high value in therapies for h-EGF deficiency-related diseases. The expression of the h-EGF gene was designed by using the pET21b(+) vector and Escherichia coli BL21(DE3) as the expression host. In a previous study, the sequence of a 6xHis tag without any restriction sites was fused to the h-EGF gene, yet it was not possible to obtain a purified and single rh-EGF by this approach. In this study, we modified the rh-EGF expression vector using site-directed mutagenesis (SDM) to remove the sequence of the 6xHis tag. The vector modification was carried out by inserting a stop codon and the EcoRI restriction site, along with deleting the 6xHis tag sequence. The results of PCR showed non-specific bands, while 2-step cycles PCR produced one non-specific band, and 3-step cycles PCR produced two non-specific bands. After purification of the PCR products, the SDM-recombinant plasmids treated for template plasmid-free product were transformed into E. coli DH5a. Even though the transformation efficiency was low, the planned gene mutations including the deletion of the 6xHis tag and insertion of the stop codon and EcoRI restriction site in plasmid pET21b(+) were successfully carried out. When using this modified vector in expression studies, rh-EGF of a similar size to that of the rh-EGF standard and approximately 1 kDa smaller than the rh-EGF-6xHis of the previous study was obtained.

Sign in / Sign up

Export Citation Format

Share Document