Role of substance P in the antidromic vasodilation, neurogenic plasma extravasation and disruption of the blood-aqueous barrier in the rabbit eye

1982 ◽  
Vol 321 (4) ◽  
pp. 329-335 ◽  
Author(s):  
Johan Stjernschantz ◽  
Marvin Sears ◽  
Hiromu Mishima
Keyword(s):  
Substance P ◽  
Plasma Extravasation ◽  
Rabbit Eye ◽  
Blood Aqueous Barrier ◽  
Antidromic Vasodilation ◽  
Neurogenic Plasma Extravasation

NK1 receptors mediate tachykinin-induced increase in microvascular clearance in hamster cheek pouch

1993 ◽  
Vol 265 (2) ◽  
pp. H593-H598
Author(s):  
X. P. Gao ◽  
R. A. Robbins ◽  
R. M. Snider ◽  
J. Lowe ◽  
S. I. Rennard ◽  
...  
Keyword(s):  
Substance P ◽  
Fluorescein Isothiocyanate ◽  
Cheek Pouch ◽  
Neurokinin A ◽  
Plasma Extravasation ◽  
Hamster Cheek Pouch ◽  
Neurokinin B ◽  
Dextran 70 ◽  
Fluorescein Isothiocyanate Dextran ◽  
Neurogenic Plasma Extravasation

The purpose of this study was to determine the receptor subtype(s) that mediates tachykinin-induced neurogenic plasma extravasation in the hamster cheek pouch. Changes in microvascular clearance were quantified by counting the number of leaky sites and calculating the clearance of fluorescein isothiocyanate-dextran [mol wt 70,000 (Dextran 70)] during suffusion of the cheek pouch with substance P, neurokinin A, neurokinin B, and capsaicin. Suffusion of substance P, capsaicin, and neurokinin A, but not neurokinin B, was associated with a significant concentration-dependent increase in leaky site formation and clearance of fluorescein isothiocyanate-Dextran 70 (P < 0.05). However, the responses to substance P and capsaicin were significantly greater than those to neurokinin A. Pretreatment with the selective, nonpeptide NK1 receptor antagonist, CP-96,345, significantly attenuated substance P- and capsaicin-induced but not neurokinin A-induced responses (P < 0.05). These effects were specific, since the 2R,3R enantiomer, CP-96,344, was inactive, and CP-96,345 had no significant effect on adenosine-induced responses. We conclude that, in the hamster cheek pouch, NK1 receptors are the predominant receptors that mediate neurogenic plasma extravasation.

Role of Neutral Endopeptitidase (NEP) and Substance P in Cutaneous Inflammation: Increased Plasma Extravasation in NEP-Deficient Mice.

Dermatitis ◽  
2001 ◽  
Vol 12 (1) ◽  
pp. 59
Author(s):  
M. Steinhoff ◽  
T. S. Cholzen ◽  
T. Luger ◽  
C. A. Armstrong ◽  
N. W. Bunnett ◽  
...  
Keyword(s):  
Substance P ◽  
Plasma Extravasation ◽  
Cutaneous Inflammation ◽  
Deficient Mice

Role of Neutral Endopeptitidase (NEP) and Substance P in Cutaneous Inflammation: Increased Plasma Extravasation in NEP-Deficient Mice.

2001 ◽  
Vol 12 (1) ◽  
pp. 59
Author(s):  
M. Steinhoff ◽  
T. S. Cholzen ◽  
T. Luger ◽  
C. A. Armstrong ◽  
N. W. Bunnett ◽  
...  
Keyword(s):  
Substance P ◽  
Plasma Extravasation ◽  
Cutaneous Inflammation ◽  
Deficient Mice

Role of endothelin in mediating neurogenic plasma extravasation in rat dura mater

Pain ◽  
1996 ◽  
Vol 64 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Peter Brändli ◽  
Bernd-Michael Löffler ◽  
Volker Breu ◽  
Rolf Osterwalder ◽  
Jean-Paul Maire ◽  
...  
Keyword(s):  
Dura Mater ◽  
Plasma Extravasation ◽  
Neurogenic Plasma Extravasation

scholarly journals A9 DORSAL ROOT GANGLIA NEURONAL RESPONSES AND SUBSTANCE P PRODUCTION ARE HIGHER IN MALE MICE

2021 ◽  
Vol 4 (Supplement_1) ◽  
pp. 10-11
Author(s):  
J Pujo ◽  
G De Palma ◽  
J Lu ◽  
S M Collins ◽  
P Bercik
Keyword(s):  
Abdominal Pain ◽  
Substance P ◽  
Gut Microbiota ◽  
Sensory Neurons ◽  
Dorsal Root ◽  
Neuronal Response ◽  
Neuronal Responses ◽  
Visceral Sensitivity ◽  
Germ Free

Abstract Background Abdominal pain is a common complaint in patients with chronic gastrointestinal disorders. Accumulating evidence suggests that gut microbiota is an important determinant of gut function, including visceral sensitivity. Germ-free (GF) mice have been shown to display visceral hypersensitivity, which normalizes after colonization. Sex also appears to play a key role in visceral sensitivity, as women report more abdominal pain than men. Thus, both gut bacteria and sex are important in the regulation of gut nociception, but the underlying mechanisms remain poorly understood. Aims To investigate the role of gut microbiota and sex in abdominal pain. Methods We used primary cultures of sensory neurons from dorsal root ganglia (DRG) of female and male conventionally raised (SPF) or germ-free (GF) mice (7–18 weeks old). To study the visceral afferent activity in vitro, calcium mobilization in DRG sensory neurons was measured by inverted fluorescence microscope using a fluorescent calcium probe Fluo-4 (1mM). Two parameters were considered i) the percentage of responding neurons ii) the intensity of the neuronal response. First, DRG sensory neurons were stimulated by a TRPV1 agonist capsaicin (12.5nM, 125nM and 1.25µM) or by a mixture of G-protein coupled receptors agonist (GPCR: bradykinin, histamine and serotonin; 1µM, 10µM and 100µM). We next measured the neuronal production of substance P and calcitonin gene-related peptide (CGRP), two neuropeptides associated with nociception, in response to capsaicin (1.25µM) or GPCR agonists (100µM) by ELISA and EIA, respectively. Results The percentage of neurons responding to capsaicin and GPCR agonists was similar in male and female SPF and GF mice. However, the intensity of the neuronal response was higher in SPF male compared to SPF female in response to capsaicin (125nM: p=0.0336; 1.25µM: p=0.033) but not to GPCR agonists. Neuronal activation was similar in GF and SPF mice of both sexes after administration of capsaicin or GPCR agonists. Furthermore, substance P and CGRP production by sensory neurons induced by capsaicin or GPCR agonists was similar in SPF and GF mice, regardless of sex. However, while the response to capsaicin was similar, the GPCR agonists-induced production of substance P was higher in SPF male mice compared to SPF females (p=0.003). The GPCR agonists-induced production of CGRP was similar in SPF male and female mice. Conclusions Our data suggest that at the level of DRG neurons, the absence of gut microbiota does not predispose to visceral hypersensitivity. The intensity of DRG neuronal responses to capsaicin and the GPCR agonists-induced production of substance P are higher in male compared to female mice, in contrast to previously published studies in various models of acute and chronic pain. Further studies are thus needed to investigate the role of sex in visceral sensitivity. Funding Agencies CIHR

Sequence of events in substance P-mediated plasma extravasation in rat skin

1989 ◽  
Vol 500 (1-2) ◽  
pp. 256-262 ◽  
Author(s):  
Zeinab Khalil ◽  
Robert D. Helme
Keyword(s):  
Substance P ◽  
Plasma Extravasation ◽  
Rat Skin ◽  
Sequence Of Events
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