Enzymatic cleavage prior to antibody incubation as a method for neuropeptide immunocytochemistry

1982 ◽  
Vol 74 (4) ◽  
pp. 457-467 ◽  
Author(s):  
R. Martin ◽  
M. Sch�fer ◽  
K. H. Voigt
Keyword(s):  
Neuropeptide Immunocytochemistry ◽  
Enzymatic Cleavage

Self-Assembling Micelles Based on an Intrinsically Disordered Protein Domain

2018 ◽  
Author(s):  
Sarah Klass ◽  
Matthew J. Smith ◽  
Tahoe Fiala ◽  
Jessica Lee ◽  
Anthony Omole ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Fusion Proteins ◽  
Organic Molecules ◽  
Intrinsically Disordered Protein ◽  
Protein Domain ◽  
Enzymatic Cleavage ◽  
Intrinsically Disordered ◽  
Disordered Protein ◽  
Self Assembling ◽  
Protein Tag

Herein, we describe a new series of fusion proteins that have been developed to self-assemble spontaneously into stable micelles that are 27 nm in diameter after enzymatic cleavage of a solubilizing protein tag. The sequences of the proteins are based on a human intrinsically disordered protein, which has been appended with a hydrophobic segment. The micelles were found to form across a broad range of pH, ionic strength, and temperature conditions, with critical micelle concentration (CMC) values below 1 µM being observed in some cases. The reported micelles were found to solubilize hydrophobic metal complexes and organic molecules, suggesting their potential suitability for catalysis and drug delivery applications.

An investigation of the enzymatic cleavage of κ-casein in non-coagulating milk

2020 ◽  
Vol 109 ◽  
pp. 104754
Author(s):  
Kajsa Nilsson ◽  
Ahmed Abdelghani ◽  
Stephen Burleigh ◽  
Lene Buhelt Johansen ◽  
Helena Lindmark-Månsson ◽  
...  
Keyword(s):  
Enzymatic Cleavage

scholarly journals S-Alkylcysteinase: Enzymatic Cleavage of S-Methyl-l-cysteine and Its Sulfoxide

1963 ◽  
Vol 238 (4) ◽  
pp. 1441-1446
Author(s):  
Junichi Nomura ◽  
Yasutomi Nishizuka ◽  
Osamu Hayaishi
Keyword(s):  
Enzymatic Cleavage

scholarly journals ENZYMATIC CLEAVAGE OF THIOETHERS

1950 ◽  
Vol 186 (1) ◽  
pp. 287-296
Author(s):  
Francis. Binkley
Keyword(s):  
Enzymatic Cleavage

scholarly journals THE ENZYMATIC CLEAVAGE OF CANAVANINE TO O-UREIDOHOMOSERINE AND AMMONIA

1957 ◽  
Vol 226 (1) ◽  
pp. 485-495 ◽  
Author(s):  
Hayato Kihara ◽  
Esmond E. Snell
Keyword(s):  
Enzymatic Cleavage

scholarly journals The Enzymatic Cleavage of Phosphate Termini from Polynucleotides

1967 ◽  
Vol 242 (5) ◽  
pp. 936-950
Author(s):  
Andrew Becker ◽  
Jerard Hurwitz
Keyword(s):  
Enzymatic Cleavage

scholarly journals The Enzymatic Cleavage of Canavanine to Homoserine and Hydroxyguanidine

1958 ◽  
Vol 233 (5) ◽  
pp. 1175-1178
Author(s):  
G.D. Kalyankar ◽  
Miyoshi Ikawa ◽  
Esmond E. Snell
Keyword(s):  
Enzymatic Cleavage
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