Localization of acid phosphatase in lysosomes of pituitary folliculo-stellate cells following estrogen withdrawal from primed male rats

1989 ◽  
Vol 91 (6) ◽  
pp. 483-485 ◽  
Author(s):  
C. W. Reifel ◽  
S. H. Shin ◽  
J. C. Stokreef
Keyword(s):  
Acid Phosphatase ◽  
Stellate Cells ◽  
Male Rats ◽  
Estrogen Withdrawal

CYTOLOGICAL AND CYTOCHEMICAL COMPARATIVE STUDY ON THE EFFECT OF CYPROTERONE (ANTI-ANDROGEN) AND GONADECTOMY ON THE GONADOTROPHIC CELLS OF THE HYPOPHYSIS IN MALE RATS

1969 ◽  
Vol 61 (2) ◽  
pp. 293-301 ◽  
Author(s):  
Kazimierz Mietkiewski ◽  
Ludwik Malendowicz ◽  
Andrzej Lukaszyk
Keyword(s):  
Acid Phosphatase ◽  
Comparative Study ◽  
Adaptive Systems ◽  
Competitive Inhibitor ◽  
Anterior Lobe ◽  
Male Rats ◽  
Mucoid Cells ◽  
Gonadotrophic Cells

ABSTRACT Cyproterone, a competitive inhibitor of testosterone, injected into male rats in daily doses of 10 mg, induces hypertrophy and hyperplasia of the mucoid cells of the hypophysis, followed by the appearance of castration cells. The largest number of these cells is observed on the 12th test-day. These changes are accompanied by degranulation of mucoid cells and an increase in the reactions for acid phosphatase and non-specific esterases. As the injection of cyproterone is continued, the number of castration cells in the anterior lobe of the hypophysis decreases and the cytoenzymic reactions observed show a fall in intensity. Gonadectomy, on the contrary, brings about lasting characteristic changes in the anterior lobe of the hypophysis, which mainly consist in the occurrence of castration cells accompanied by an increase in the intensity of reactions for acid phosphatase and non-specific esterases. The above data suggest that in the course of administration of cyproterone, suitable adaptive systems develop in the organism which inhibit the action of this anti-androgen.

Effect of 6-wk estrogen withdrawal or replacement on myocardial ischemic tolerance in rats

2000 ◽  
Vol 278 (4) ◽  
pp. H1030-H1034 ◽  
Author(s):  
Patrick H. McNulty ◽  
Dinesh Jagasia ◽  
Jennifer M. Whiting ◽  
Teresa Caulin-Glaser
Keyword(s):  
Ventricular Tachycardia ◽  
Coronary Occlusion ◽  
Myocardial Infarct ◽  
Menopausal Status ◽  
Left Ventricular ◽  
Male Rats ◽  
Myocardial Infarct Size ◽  
Risk Area ◽  
Estrogen Withdrawal

Menopausal status is a risk factor for coronary artery disease death, but the mechanism underlying this association is uncertain. To test whether estrogen ameliorates the effects of acute myocardial ischemia in ways likely to translate into a mortality difference, we compared the response to brief (6-min) and prolonged (45-min) coronary occlusion in vivo in five groups (each n = 16) of rats: ovariectomized females; ovariectomized females after 6 wk 17β-estradiol replacement; male rats supplemented with estradiol for 6 wk; normal males; and normal females. Coronary occlusion produced a uniform ischemic risk area averaging 53 ± 3% of left ventricular volume. After a brief occlusion, reperfusion ventricular tachycardia/fibrillation occurred with >85% frequency in all groups. During a prolonged occlusion, ischemic ventricular tachycardia occurred in 100% and sustained tachycardia requiring cardioversion in >75% of rats in all groups. Myocardial infarct size averaged 52 ± 4% of the ischemic risk area and was similarly unaffected by gender or estrogen status. We conclude that neither short-term estrogen withdrawal, replacement, nor supplementation significantly affects the potentially lethal outcomes from acute coronary occlusion in this species.

Protective effects of selenium and nano-selenium on bisphenol-induced reproductive toxicity in male rats

2018 ◽  
Vol 38 (4) ◽  
pp. 398-408 ◽  
Author(s):  
AA Khalaf ◽  
WMS Ahmed ◽  
WA Moselhy ◽  
BR Abdel-Halim ◽  
MA Ibrahim
Keyword(s):  
Acid Phosphatase ◽  
Reproductive Toxicity ◽  
Prostatic Acid Phosphatase ◽  
Negative Control ◽  
Consumer Products ◽  
Male Rats ◽  
Fragmentation Pattern ◽  
Protective Effects ◽  
Protective Agents

Bisphenol A (BPA) is a widespread compound associated with the manufacture of many consumer products. The BPA-induced reproductive toxicity was reported to be mainly attributed to oxidative stress. However, the role of antioxidants usage to decrease the injurious effects of BPA, on male reproductive functions, remains to unveil. The present research is established to evaluate the role of selenium (Se) and its nano form (NSe) as protective agents to alleviate BPA-induced testicular toxicity. Ninety mature albino male rats were assigned into six equal groups: negative control; orally BPA 150 mg/kg; Se 3 mg/kg; NSe 2 mg/kg; both BPA 150 mg/kg and Se 3 mg/kg; and BPA 150 mg/kg + NSe 2 mg/kg. The experiment lasted for 70 consecutive days, and then serum was collected for estimation of prostatic acid phosphatase. Testicular tissues were subjected to measurement of antioxidant status, lipid peroxidation, DNA damage, and expression of some apoptotic genes. Our results reported that BPA-induced marked testicular damage evidenced by significant elevations in serum prostatic acid phosphatase activity, malondialdehyde levels, a decrease in testicular catalase activity and reduced glutathione level. Moreover, marked DNA internucleosomal fragmentation pattern as well as upregulation of cyclooxygenase-2 and estrogen receptor-2 NSe genes were detected. Coadministration of Se and NSe attenuated the reproductive toxicity induced by BPA via improvement of the antioxidant activity, genetic changes, and restoration of testicular tissue nearly as control one. These results indicated that both Se and NSe forms could be used as reproductive protective agents against the detrimental effect induced by BPA. However, the NSe surpassed the selenium in modulating the DNA laddering, and the studied gene expression levels, and offered a potent reproductive protection.

scholarly journals HEMOPOESIS AND FUNCTIONAL CONDITION OF BLOOD LEUKOCYTES DURING INFLAMMATION IN THE BACKGROUND OF LOCAL REMOVAL OF NONPHOCOTATING GRANULOZITES

2020 ◽  
Vol 74 (2) ◽  
pp. 153-156
Author(s):  
A.G. Sirak ◽  
◽  
E.I. Piskareva ◽  
M.A. Dolgashova ◽  
O.G. Magomedova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acid Phosphatase ◽  
Functional Status ◽  
Functional Activity ◽  
Functional Condition ◽  
Male Rats ◽  
Marker Enzymes ◽  
Blood Leukocytes ◽  
Experimental Conditions ◽  
Wistar Male Rats

The article is devoted to the functional state of blood leukocytes and peculiarities of hematopoiesis during inflammation under experimental conditions against the background of local removal of non-phagocytic granulocytes. The functional status of blood leukocytes obtained from 24 Wistar male rats weighing 180–220 g was judged by the activity of their marker enzymes. Myeloperoxidase (MPO) and acid phosphatase (CF), macrophage monocytes – α-naphthylacetate esterase (α-NAE), lymphocytes – CF and α-NAE served as markers of the functional activity of neutrophils. Found that during inflammation on the background of prior removal of NG from the focus, there is less neutrophil and monocyte entry from the bone marrow into the blood and further into the inflammation focus, changes in the intensity and dynamics of hemopoiesis, and as a result, increased lymphocyte degranulation.

scholarly journals DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY USING 1-ACETYL-3-INDOLYL PHOSPHATE AS SUBSTRATE

1971 ◽  
Vol 19 (3) ◽  
pp. 175-181 ◽  
Author(s):  
MASANDO HAYASHI
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Lead Ions ◽  
Lead Salt ◽  
Male Rats ◽  
Cytochemical Demonstration ◽  
Liver And Kidney ◽  
Triton X

A simultaneous coupling azo-indoxyl method for the cytochemical demonstration of acid phosphatase activity using p-toluidine salt of 1-acetyl-3-indolyl phosphate is described. A satisfactory staining for the enzyme activity was obtained following incubation of formol-calcium-fixed frozen sections for 30 min at 25°C in a medium containing 1 mM each of the substrate and hexazonium pararosanilin and adjusted to pH 4.5-5.0 with acetate buffer. The distribution of acid phosphatase activity demonstrated by this method was identical with that obtained either by Gomori's technique using β-glycerophosphate as substrate or by the Barka and Anderson's naphthol AS-BI phosphate-hexazonium pararosanilin method in several tissues of male rats so far examined. However, the adrenal enzyme activity was most prominent in the medulla with 1-acetyl-3-indolyl phosphate and β-glycerophosphate but it was more marked in the cortex with naphthol AS-BI phosphate. An advantage of using 1-acetyl-3-indolyl phosphate as substrate is that the same compound can be used for comparing azo-indoxyl and lead-salt methods. Effects of phospholipase C and Triton X-100 on staining for acid phosphatase were tested by pretreating fixed rat liver and kidney sections with these agents and incubating them in the medium containing 1-acetyl-3-indolyl phosphate and either hexazonium pararosanilin or lead ions as a coupler. The pretreatment did not change discrete lysosomal staining, as seen in untreated controls, using pararosanilin as a coupler, but greatly modified the staining using lead ions. The results indicate that the preciseness of staining for acid phosphatase with lead-salt method is highly dependent on some lipid material which attracts lead in tissues and that appropriately devised azo dye or azo-indoxyl methods demonstrate enzyme sites more accurately than lead-salt method.

Effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b in male rats

2011 ◽  
Vol 236 (11) ◽  
pp. 1298-1305 ◽  
Author(s):  
Xiao Chen ◽  
Guoying Zhu ◽  
Chunlin Shao ◽  
Taiyi Jin ◽  
Mingguang Tan ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Bone Mineral ◽  
Bone Microstructure ◽  
Male Rats ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Sprague Dawley ◽  
Mineral Density ◽  
Microstructure Parameters ◽  
Tracp 5B

This study investigated the effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b (Tracp 5b) in male rats. Sprague-Dawley male rats were divided into three groups that were given CdCl2 by subcutaneous injection at doses of 0, 0.1 and 0.5 mg/kg body weight (bw) for 12 weeks, respectively. Before killing at the 12th week, microcomputed tomography scanning was performed on the proximal tibia, and urine samples were collected from all of the rats. All rats were then killed, and their blood was collected for biomarkers assay. Bone tissues were dissected for mineral density determinations and histology. The concentration of cadmium in the blood, urine and bone of rats treated with cadmium were significantly higher than in the control group. The bone mineral density, bone mineral concentrations and bone microstructure index of rats treated with cadmium at 0.5 mg/kg bw were clearly lower than in the control rats. Histological investigation also revealed damage to the bone microstructure caused by cadmium. Tracp 5b concentrations in rats treated with cadmium were dose dependently higher than the control. The concentration of cadmium in blood, urine and bone was significantly correlated with Tracp 5b and bone microstructure parameters. Cadmium was shown to induce bone microstructure damage, especially to trabecular bone. The elevated concentrations of serum Tracp 5b suggest that bone resorption mediated via osteoclasts is an important mechanism for the toxic effects of cadmium on bone.

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