Effect of the level of the carbon source on the activity of some lytic enzymes released during autolysis of Aspergillus niger

1977 ◽  
Vol 62 (1) ◽  
pp. 23-30 ◽  
Author(s):  
P. G�mez ◽  
Fuensanta Reyes ◽  
R. Lahoz
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Lytic Enzymes

scholarly journals Gold Bioleaching from Printed Circuit Boards of Mobile Phones by Aspergillus niger in a Culture without Agitation and with Glucose as a Carbon Source

Metals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 521 ◽  
Author(s):  
Rosalba Argumedo-Delira ◽  
Mario J. Gómez-Martínez ◽  
Brenda Joan Soto
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Culture Medium ◽  
Printed Circuit Boards ◽  
Biological Processes ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Material Resources ◽  
Low Energy Consumption ◽  
Fungal Consortium

Hydrometallurgical and pyrometallurgical processes to recover gold (Au) from cell-phone printed circuit boards (PCBs) have the disadvantage of generating corrosive residues and consuming a large amount of energy. Therefore, it is necessary to look for biological processes that have low energy consumption and are friendly to the environment. Among the biological alternatives for the recovery of Au from PCB is the use of cyanogenic bacteria and filamentous fungi in cultures with agitation. Considering that it is important to explore the response of microorganisms in cultures without agitation to reduce energy expenditure in the recovery of metals from PCB, the present investigation evaluated the capacity of Aspergillus niger MXPE6 and a fungal consortium to induce Au bioleaching from PCB in a culture medium with glucose as a carbon source and without agitation (pH 4.5). The results indicate that the treatments with PCB inoculated with the fungal consortium showed a considerable decrease in pH (2.8) in comparison with the treatments inoculated with A. niger MXPE6 (4.0). The fungal consortium showed a significantly higher Au bioleaching (56%) than A. niger MXPE6 (17%). Finally, the use of fungal consortia grown without agitation could be an alternative to recover metals from PCB, saving energy and material resources.

scholarly journals Bioconversion of Mango Pulp Industrial Waste into Ellagic acid Using Aspergillus niger

2020 ◽  
Author(s):  
Athiappan Murugan ◽  
Anandan Rubavathi ◽  
Visali Kannan ◽  
Aurumugam Parthiban
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Ellagic Acid ◽  
Acid Production ◽  
Bioactive Compound ◽  
Ft Ir ◽  
Ir Analysis ◽  
Pulp Processing ◽  
Mango Pulp ◽  
Plant Sources

AbstractEllagic acid was considered as the potential bioactive compound with many therapeutical applications. Bioconversion of tannin present in the mango pulp processing waste in to ellagic acid using fungi would be better alternate than the chemical as well as extraction from plant sources. A total of three different fungi were isolated from the soil sample and it was confirmed as Aspergillus niger. Further, the isolated strains of A. niger were identified to produce ellagic acid from ellagitannin of mango waste. Quantification of the ellagic acid production was carried out by solid-state fermentation using 3% of mango waste as substrate. Ellagic acid enzyme activity was calculated and found to be 17.6 U ml−1 The ellagic acid production was optimized to fix the various factors, that is, pH and temperature, nitrogen and carbon source. The maximum production (200 μg/g) of ellagic acid was achieved at pH 5.5, temperature 30 °C, Ammonium nitrate as nitrogen source, 0.2% of NaCl and carbon source (0.2% of sugar) with 3% of mango pulp waste. Ellagic acid produced was characterized by UV–vis spectrophotometer and by FT-IR analysis.

scholarly journals Mannitol Is Required for Stress Tolerance in Aspergillus niger Conidiospores

2003 ◽  
Vol 2 (4) ◽  
pp. 690-698 ◽  
Author(s):  
George J. G. Ruijter ◽  
Maarten Bax ◽  
Hema Patel ◽  
Simon J. Flitter ◽  
Peter J. I. van de Vondervoort ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Cell Damage ◽  
Reducing Power ◽  
Dry Weight ◽  
Stress Conditions ◽  
Prolonged Storage ◽  
Developmentally Regulated ◽  
In The Wild ◽  
Major Metabolic Pathway

ABSTRACT d-Mannitol is the predominant carbon compound in conidiospores of the filamentous fungus Aspergillus niger and makes up 10 to 15% of the dry weight. A number of physiological functions have been ascribed to mannitol, including serving as a reserve carbon source, as an antioxidant, and to store reducing power. In this study, we cloned and characterized the A. niger mpdA gene, which encodes mannitol 1-phosphate dehydrogenase (MPD), the first enzyme in the mannitol biosynthesis pathway. The mpdA promoter contains putative binding sites for the development-specific transcription factors BRLA and ABAA. Furthermore, increased expression of mpdA in sporulating mycelium suggests that mannitol biosynthesis is, to a certain extent, developmentally regulated in A. niger. Inactivation of mpdA abolished mannitol biosynthesis in growing mycelium and reduced the mannitol level in conidiospores to 30% that in the wild type, indicating that MPD and mannitol 1-phosphate phosphatase form the major metabolic pathway for mannitol biosynthesis in A. niger. The viability of spores after prolonged storage and germination kinetics were normal in an mpdA null mutant, indicating that mannitol does not play an essential role as a reserve carbon source in A. niger conidia. However, conidiospores of a ΔmpdA strain were extremely sensitive to a variety of stress conditions, including high temperature, oxidative stress and, to a lesser extent, freezing and lyophilization. Since mannitol supplied in the medium during sporulation repaired this deficiency, mannitol appears to be essential for the protection of A. niger spores against cell damage under these stress conditions.

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