Biosynthesis of the major urinary proteins in mouse liver: A biochemical genetic study

1981 ◽  
Vol 19 (11-12) ◽  
pp. 1261-1273 ◽  
Author(s):  
Franklin G. Berger ◽  
Paula Szoka
Keyword(s):  
Genetic Study ◽  
Mouse Liver ◽  
Urinary Proteins ◽  
Major Urinary Proteins ◽  
Biochemical Genetic

scholarly journals Differential expression in male and female mouse liver of very similar mRNAs specified by two group 1 major urinary protein genes.

1989 ◽  
Vol 9 (5) ◽  
pp. 2202-2207 ◽  
Author(s):  
I McIntosh ◽  
J O Bishop
Keyword(s):  
Mouse Liver ◽  
Hormonal Status ◽  
Oligonucleotide Probes ◽  
Urinary Proteins ◽  
Pairwise Similarity ◽  
Major Urinary Protein ◽  
Male And Female ◽  
Major Urinary Proteins ◽  
Different Levels ◽  
Group 1

The major urinary proteins of the mouse are encoded by a large multigene family composed of several distinct groups of genes distinguished by differences in sequence and expression characteristics. The genes in the largest group (group 1) show greater than 99% pairwise similarity in their exons. By hybridization between RNA and a specifically designed oligonucleotide, we confirmed that genes of this group are expressed mainly in the liver. By using additional gene-specific oligonucleotide probes, we have been able to distinguish between the species of mRNA corresponding to two of these genes and to measure their abundance in male and female liver. Both mRNAs are present in male liver at high but different levels. Both are also present in female liver, one at a much lower level than in the male and the second at a very low level indeed. Both are present at male levels in the livers of females induced with testosterone. These results show unequivocally that the expression of different group 1 Mup genes is differentially influenced by the hormonal status of the mouse.

Differential expression in male and female mouse liver of very similar mRNAs specified by two group 1 major urinary protein genes

1989 ◽  
Vol 9 (5) ◽  
pp. 2202-2207
Author(s):  
I McIntosh ◽  
J O Bishop
Keyword(s):  
Mouse Liver ◽  
Hormonal Status ◽  
Oligonucleotide Probes ◽  
Urinary Proteins ◽  
Pairwise Similarity ◽  
Major Urinary Protein ◽  
Male And Female ◽  
Major Urinary Proteins ◽  
Different Levels ◽  
Group 1

The major urinary proteins of the mouse are encoded by a large multigene family composed of several distinct groups of genes distinguished by differences in sequence and expression characteristics. The genes in the largest group (group 1) show greater than 99% pairwise similarity in their exons. By hybridization between RNA and a specifically designed oligonucleotide, we confirmed that genes of this group are expressed mainly in the liver. By using additional gene-specific oligonucleotide probes, we have been able to distinguish between the species of mRNA corresponding to two of these genes and to measure their abundance in male and female liver. Both mRNAs are present in male liver at high but different levels. Both are also present in female liver, one at a much lower level than in the male and the second at a very low level indeed. Both are present at male levels in the livers of females induced with testosterone. These results show unequivocally that the expression of different group 1 Mup genes is differentially influenced by the hormonal status of the mouse.

Heterogeneity of Major Urinary Proteins in House Mice: Population and Sex Differences

2001 ◽  
pp. 233-240 ◽  
Author(s):  
Caroline E. Payne ◽  
Nick Malone ◽  
Rick Humphries ◽  
Carl Bradbrook ◽  
Christina Veggerby ◽  
...  
Keyword(s):  
Sex Differences ◽  
House Mice ◽  
Urinary Proteins ◽  
Major Urinary Proteins

Multiple genes coding for the androgen-regulated major urinary proteins of the mouse

Cell ◽  
1979 ◽  
Vol 17 (2) ◽  
pp. 449-457 ◽  
Author(s):  
Nicholas D. Hastie ◽  
William A. Held ◽  
John J. Toole
Keyword(s):  
Urinary Proteins ◽  
Major Urinary Proteins ◽  
Multiple Genes

scholarly journals Diversity of major urinary proteins (MUPs) in wild house mice

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Michaela Thoß ◽  
Viktoria Enk ◽  
Hans Yu ◽  
Ingrid Miller ◽  
Kenneth C. Luzynski ◽  
...  
Keyword(s):  
House Mice ◽  
Urinary Proteins ◽  
Major Urinary Proteins ◽  
Wild House Mice

scholarly journals In Contrast to Dietary Restriction, Application of Resveratrol in Mice Does not Alter Mouse Major Urinary Protein Expression

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 815 ◽  
Author(s):  
Kathrin Pallauf ◽  
Ilka Günther ◽  
Dawn Chin ◽  
Gerald Rimbach
Keyword(s):  
Protein Expression ◽  
Dietary Restriction ◽  
Model Organisms ◽  
Mrna Levels ◽  
Urinary Proteins ◽  
Pheromone Communication ◽  
Beneficial Effects ◽  
Major Urinary Proteins ◽  
Glucocorticoid Response ◽  
Glucocorticoid Response Elements

Resveratrol (RSV) supplementation in mice has been discussed as partly mimicking the beneficial effects of dietary restriction (DR). However, data on putative benefits from resveratrol application in mice and other model organisms including humans is contradictory. Mouse major urinary proteins (MUPs) are a family of proteins that are expressed in rodent liver and secreted via urine. Impacting (mating) behavior and pheromone communication, they are severely down-regulated upon DR. We carried out two studies in C57BL/6Rj mice where RSV was either supplemented via diet or injected intraperitoneally for 8 weeks. Contrary to −40% DR, RSV did not decrease total MUP protein expression or Mup (amongst others Mup3, Mup5, Mup6, Mup15, and Mup20) mRNA levels in mouse liver when compared to ad-libitum (AL)-fed controls. Since inhibitory glucocorticoid response elements can be found in Mup promoters, we also measured glucocorticoid receptor (GR) levels in nuclear hepatic extracts. Consistent with differential MUP expression, we observed more nuclear GR in DR mice than in RSV-supplemented and AL control mice with no difference between RSV and AL. These findings point to the notion that, in mice, RSV does not mimic DR in terms of differential MUP expression.

scholarly journals Individual odour signatures that mice learn are shaped by involatile major urinary proteins (MUPs)

BMC Biology ◽  
2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Sarah A. Roberts ◽  
Mark C. Prescott ◽  
Amanda J. Davidson ◽  
Lynn McLean ◽  
Robert J. Beynon ◽  
...  
Keyword(s):  
Urinary Proteins ◽  
Major Urinary Proteins

scholarly journals Structural genes of the mouse major urinary protein are on chromosome 4.

1982 ◽  
Vol 94 (2) ◽  
pp. 414-417 ◽  
Author(s):  
K Krauter ◽  
L Leinwand ◽  
P D'Eustachio ◽  
F Ruddle ◽  
J E Darnell
Keyword(s):  
Protein Synthesis ◽  
Sequence Analysis ◽  
Chromosome 4 ◽  
Structural Genes ◽  
Urinary Proteins ◽  
Coding Region ◽  
Somatic Cell Genetics ◽  
Major Urinary Protein ◽  
Major Urinary Proteins ◽  
Trans Regulation

The major urinary proteins (MUPs) of mouse are a family of at least three major proteins which are synthesized in the liver of all strains of mice. The relative levels of synthesis of these proteins with respect to each other in the presence of testosterone is regulated by the Mup-a locus located on chromosome 4. In an effort to determine the mechanism of this regulation in molecular terms, a cDNA clone containing most of the coding region of a MUP protein has been isolated and identified by partial DNA sequence analysis. Using a combination of hybridization analysis and somatic cell genetics, the structural gene family has been unambiguously mapped to mouse chromosome 4. These data suggest that Mup-a regulation operates in a cis fashion and that models proposing trans regulation of MUP protein synthesis are unlikely.

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