Polymorphism of rat seminal vesicle secretory proteins: Characterization of svp-1 and svp-2 and their identification with the major secretory proteins IV and V

1984 ◽  
Vol 22 (5-6) ◽  
pp. 567-576 ◽  
Author(s):  
A. Maffei ◽  
G. Paonessa ◽  
P. Abrescia ◽  
S. Metafora ◽  
J. Guardiola
Keyword(s):  
Seminal Vesicle ◽  
Secretory Proteins ◽  
Rat Seminal Vesicle

scholarly journals Effects of testosterone on messenger ribonucleic acid and protein synthesis in rat seminal vesicle

1978 ◽  
Vol 174 (2) ◽  
pp. 543-551 ◽  
Author(s):  
Stephen J. Higgins ◽  
Joy M. Burchell
Keyword(s):  
Protein Synthesis ◽  
Seminal Vesicle ◽  
Steroid Hormones ◽  
Secretory Proteins ◽  
Total Acid ◽  
Free System ◽  
Messenger Ribonucleic Acid ◽  
Mrna Population ◽  
Rat Seminal Vesicle

In a previous report [Higgins et al. (1976) Biochem. J.158, 271–282] we described the effects of alterations in androgen status on the synthesis of two basic secretory proteins of the rat seminal vesicle. In the present paper we examine the effects of testosterone on the activity of mRNA in the seminal vesicle. Total cellular poly(A)-rich RNA was isolated and translated in a cell-free system prepared from wheat germ. Translation products were separated on denaturing polyacrylamide gels and the protein bands corresponding to the two basic secretory proteins were identified immunologically. Incorporation of radioactive methionine into these bands was taken as a measure of the individual mRNA activities. Total mRNA activity was estimated by radioactivity in total acid-precipitable material. The results show that 1 to 2 weeks after castration the activities of mRNA molecules for the basic secretory proteins were decreased 10–20-fold on a tissue basis. Testosterone given in vivo rapidly and substantially restores mRNA activity to normal. Since these changes correlate closely with variations in the rates of synthesis of the secretory proteins in whole cells it suggests that androgenic steroids control protein synthesis chiefly via mRNA availability. In this respect their action resembles those of other steroid hormones acting in other systems. However, these effects of testosterone on the mRNA molecules for the major secretory proteins could not be distinguished from those on total mRNA. Thus the proportion of the total mRNA population accounted for by the two specific mRNA molecules showed less than a 2-fold variation with androgen status. Similarly the two secretory proteins always accounted for 25–33% of general protein synthesis. This is in sharp contrast with the markedly differential effects of other steroid hormones controlling synthesis of major proteins in other well-studied systems. We interpret our results as indicating that testosterone regulates the mRNA population of the seminal vesicle as a whole.

Characterization of VIP receptors coupled to adenylate cyclase in rat seminal vesicle

1989 ◽  
Vol 26 (2) ◽  
pp. 160 ◽  
Author(s):  
L. Guijarro ◽  
M.S. Rodriguez-Pena ◽  
J.C. Prieto
Keyword(s):  
Adenylate Cyclase ◽  
Seminal Vesicle ◽  
Vip Receptors ◽  
Rat Seminal Vesicle

scholarly journals Structural characterization of the rat seminal vesicle secretion II protein and gene.

1990 ◽  
Vol 265 (17) ◽  
pp. 9896-9903
Author(s):  
S E Harris ◽  
M A Harris ◽  
C M Johnson ◽  
M F Bean ◽  
J G Dodd ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Structural Characterization ◽  
Vesicle Secretion ◽  
Rat Seminal Vesicle

Characterization of the cytoplasmic androgen receptor of rat seminal vesicle

Steroids ◽  
1977 ◽  
Vol 30 (6) ◽  
pp. 751-764 ◽  
Author(s):  
Tamás Zakár ◽  
Miklós Tóth
Keyword(s):  
Androgen Receptor ◽  
Seminal Vesicle ◽  
Rat Seminal Vesicle

Expression and pharmacological characterization of α1-adrenoceptors in rat seminal vesicle

1999 ◽  
Vol 381 (2-3) ◽  
pp. 141-149 ◽  
Author(s):  
Marcos A Silva ◽  
Alexandre Megale ◽  
Maria Christina W Avellar ◽  
Catarina S Porto
Keyword(s):  
Seminal Vesicle ◽  
Pharmacological Characterization ◽  
Rat Seminal Vesicle

scholarly journals Characterization of a genomic clone for rat seminal vesicle secretory protein IV

1983 ◽  
Vol 11 (10) ◽  
pp. 3169-3186 ◽  
Author(s):  
Jagannadha C. Kandala ◽  
Malathi K. Kistler ◽  
Robert P. Lawther ◽  
W. Stephen Kistler
Keyword(s):  
Seminal Vesicle ◽  
Secretory Protein ◽  
Genomic Clone ◽  
Rat Seminal Vesicle
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