A simple procedure for the thin-layer chromatographie separation and determination of prostaglandins and other metabolites formed from 14C-arachidonic acid in human blood platelets

1980 ◽  
Vol 304 (5) ◽  
pp. 412-416 ◽  
Author(s):  
K. C. Srivastava ◽  
K. P. Tiwari
Keyword(s):  
Arachidonic Acid ◽  
Thin Layer ◽  
Human Blood ◽  
Blood Platelets ◽  
Simple Procedure ◽  
Human Blood Platelets

scholarly journals Intracellular mechanisms in the activation of human platelets by low-density lipoproteins

1987 ◽  
Vol 242 (2) ◽  
pp. 559-564 ◽  
Author(s):  
H E Andrews ◽  
J W Aitken ◽  
D G Hassall ◽  
V O Skinner ◽  
K R Bruckdorfer
Keyword(s):  
Arachidonic Acid ◽  
Human Blood ◽  
Blood Platelets ◽  
Human Platelets ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Protein L ◽  
Stimulus Response ◽  
Dense Granules ◽  
Human Blood Platelets

Low-density lipoproteins (LDL) have been shown to cause aggregation of human blood platelets at concentrations above 2 g of protein/l. The secretion of the contents of platelet dense granules was detected, but not that of the lysosomes. LDL gave rise to a mobilization of [3H]arachidonic acid from phospholipids and the appearance of products of the cyclo-oxygenase pathway after only 10 s. LDL-promoted aggregation was inhibited by both aspirin and indomethacin. There was an increase in 3H-labelled diacylglycerols and the phosphorylation of 47 kDa proteins. LDL therefore shares at least some of the mechanisms of stimulus/response coupling with those of other agonists.

Rheo-Optical Shape Analysis of Human Blood Platelets

1982 ◽  
Vol 48 (02) ◽  
pp. 204-207 ◽  
Author(s):  
Hubert Affolter ◽  
Alfred Pletscher
Keyword(s):  
Human Blood ◽  
Noise Level ◽  
Blood Platelets ◽  
Scattered Light ◽  
Quantitative Measure ◽  
Noise Levels ◽  
Optical Signals ◽  
Instrument Measuring ◽  
Human Blood Platelets

SummaryAlterations in rheo-optical signals obtained from suspensions of human blood platelets treated in various ways (drugs, storage in the cold, stirring, etc.) were monitored. A self-normalizing instrument measuring scattered light and relative amplitude of rapid oscillation of light intensity (noise level) at different angles was used. The following results have been obtained: 1. The light scattered at an angle of 40° was decreased with considerable selectivity by pseudopod formation, if aggregation was inhibited. 2. The noise levels at 0° and 40° were selectively diminished by the transition from the discoid to the spheroid shape of the platelets. 3. At 40°, but not at 0°, the noise level responded without delay and was not influenced by aggregate formation. Using this method alterations in platelet form, i.e. spheroid transformation and pseudopod formation, can be specifically and continuously monitored. In addition, the noise level at 40° is a definite quantitative measure of the discoid state. This allows the determination of platelet activation without manipulations such as changes in stirring rates or addition of drugs.

scholarly journals Identical subcellular distribution of palmitoyl-CoA and arachidonoyl-CoA synthetase activities in human blood platelets

1989 ◽  
Vol 261 (1) ◽  
pp. 71-76 ◽  
Author(s):  
A M Bakken ◽  
M Farstad
Keyword(s):  
Enzyme Activity ◽  
Arachidonic Acid ◽  
Human Blood ◽  
Blood Platelets ◽  
The Other ◽  
Heat Inactivation ◽  
Tubular System ◽  
Chain Acyl ◽  
Human Blood Platelets ◽  
Long Chain

Fractionation of human blood platelets showed that palmitoyl-CoA synthetase and arachidonoyl-CoA synthetase activities had an identical distribution among subcellular fractions. The activity was highest with arachidonic acid as substrate in all fractions, with an enzyme activity of 50 nmol/min per mg of protein, in a ‘dense-tubular-system’-enriched fraction. The ratio activities with arachidonate and palmitate as substrates was about 1.5 in all fractions. Heat inactivation did not distinguish between arachidonoyl-CoA synthetase and a palmitoyl-CoA synthetase. On the other hand, heat inactivation indicated two pools of long-chain acyl-CoA synthetases: one in a mitochondria- and one in the dense-tubular-system-enriched fraction.

Determination of the Life of Human Blood Platelets using Labelled Diisopropylfluorophosphonate

Nature ◽  
1955 ◽  
Vol 175 (4456) ◽  
pp. 552-553 ◽  
Author(s):  
C. H. W. LEEKSMA ◽  
J. A. COHEN
Keyword(s):  
Human Blood ◽  
Blood Platelets ◽  
Human Blood Platelets
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