Human milk anti-E. coli antibodies: Relationship to maternal parity

1979 ◽  
Vol 130 (3) ◽  
pp. 173-180 ◽  
Author(s):  
N. Vardinon ◽  
Z. Spirer ◽  
J. Goldhar ◽  
B. Kacevman ◽  
E. Eylan
Keyword(s):  
Human Milk ◽  
E Coli ◽  
Maternal Parity

scholarly journals 3 RELATIONSHIP BETWEEN E. coli COLONISATION FACTOR I SPECIFIC SECRETORY IgA IN HUMAN MILK AND SALIVA

1988 ◽  
Vol 24 (3) ◽  
pp. 405-405 ◽  
Author(s):  
K A Nathavitharana ◽  
D Catty ◽  
A S McNeish
Keyword(s):  
Human Milk ◽  
Secretory Iga ◽  
E Coli ◽  
Factor I

scholarly journals Fab-Independent Antiadhesion Effects of Secretory Immunoglobulin A on S-Fimbriated Escherichia coli Are Mediated by Sialyloligosaccharides

1998 ◽  
Vol 66 (8) ◽  
pp. 3971-3973 ◽  
Author(s):  
Horst Schroten ◽  
Christoph Stapper ◽  
Ricarda Plogmann ◽  
Henrik Köhler ◽  
Jörg Hacker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Human Milk ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
E Coli ◽  
Human Epithelial Cells ◽  
Early Human ◽  
Secretory Immunoglobulin

ABSTRACT S-fimbriated Escherichia coli strains cause sepsis and meningitis in newborns and are known to recognize the carbohydrate sequence sialyl-(α2-3)-galactoside. We show that adhesion of cloned S-fimbriated E. coli to human epithelial cells is inhibited Fab independently by sialyloligosaccharides on secretory immunoglobulin A (s-IgA). This indicates an anti-infective function of s-IgA (Fc), particularly in early human milk.

Effects of Microwave Radiation on Anti-infective Factors in Human Milk

PEDIATRICS ◽  
1992 ◽  
Vol 89 (4) ◽  
pp. 667-669
Author(s):  
Richard Quan ◽  
Christine Yang ◽  
Steven Rubinstein ◽  
Norman J. Lewiston ◽  
Philip Sunshine ◽  
...  
Keyword(s):  
Human Milk ◽  
High Temperatures ◽  
Low Temperatures ◽  
Marked Decrease ◽  
Lysozyme Activity ◽  
Secretory Iga ◽  
E Coli ◽  
Milk Samples ◽  
Power Setting ◽  
Coli Growth

In intensive care nurseries it has become common practice to use microwave thawing of frozen human milk for more rapid accessibility. Twenty-two freshly frozen human milk samples were tested for lysozyme activity, total IgA, and specific secretory IgA to Escherichia coli serotypes 01, 04, and 06. The samples were heated by microwave for 30 seconds at a low- or high-power setting and then reanalyzed. One-mL aliquots of 10 additional human milk samples were micro-waved at low (20°C to 25°C), medium (60°C to 70°C), and high (≥98°C) setting before the addition to each of 1 mL of diluted E coli suspension. E coli growth was determined after 3½ hours of incubation at 37°C. Microwaving at high temperatures (72°C to 98°C) caused a marked decrease in activity of all the tested anti-infective factors. E coli growth at ≥98°C was 18 times that of control human milk. Microwaving at low temperatures(20°C to 53°C) had no significant effect on total IgA, specific IgA to E coli serotypes 01 and 04, but did significantly decrease lysozyme and specific IgA to E coli serotype 06. Even at 20°C to 25°C, E coli growth was five times that of control human milk. Microwaving appears to be contraindicated at high temperatures, and questions regarding its safety exist even at low temperatures.

scholarly journals Naturally Occurring Bioactive Oligosaccharides in Goat's Milk-Infant Formula (P11-078-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Andrea Leong ◽  
Liu Zhiqian ◽  
Christopher Pillidge ◽  
Bogdan Zisu ◽  
Simone Rochfort ◽  
...  
Keyword(s):  
Human Milk ◽  
Infant Formula ◽  
Conflicts Of Interest ◽  
Growth Media ◽  
Potential Health ◽  
E Coli ◽  
Funding Sources ◽  
Naturally Occurring ◽  
Goat’S Milk ◽  
Goat's Milk

Abstract Objectives Human milk is rich in oligosaccharides (OS) that impart significant health benefits to the infant. When breast feeding is not possible, infant formula (IF) is used as an alternative. However, little is known about the presence of OS in IF and their potential health effects. This study was aimed at quantifying naturally occurring OS in a commercial goat's milk IF and examining their prebiotic and anti-infection properties. Methods OS were isolated from IF using molecular weight cut-off filters and HILIC-LC/MS system. Prebiotic activity of OS was assessed by co-incubating Bifidobacterium (BB12 and BB526) and Lactobacillus (LA4461 and LC2607) strains in growth media with or without OS anaerobically at 37°C for 24 hr and measuring OD600. Anti-adhesion potential of OS was determined by co-incubating E. coli NCTC 10418 and S. typhimurium, pre-incubated with or without OS for 2 hr at 37°C, with Caco-2 cells for 90 min. After PBS washes and cell lysis, supernatants were plated onto agars for measuring bacterial counts and calculating the anti-adhesion %. Results Fourteen OS were quantified in goat's milk IF, of which 5 were structurally similar to those reported in human milk. At concentrations similar to those in reconstituted IF, OS fractions were significantly effective in enhancing the growth of bifidobacteria and lactobacilli strains and preventing the adhesion of E. coli NCTC10418 (52.4%) and S. typhimurium (35%) to the Caco-2 cells. Conclusions Goat's milk IF contains several naturally occurring human milk-like OS that appear to promote the growth of beneficial gut microbes and protect against gut pathogens. Funding Sources No conflicts of interest were involved. Nuchev Pty Ltd. provided goat's milk IF. Research costs were covered by RMIT University.

scholarly journals Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study

2021 ◽  
Vol 11 ◽  
Author(s):  
Michelle K. McGuire ◽  
Arlo Z. Randall ◽  
Antti E. Seppo ◽  
Kirsi M. Järvinen ◽  
Courtney L. Meehan ◽  
...  
Keyword(s):  
Human Milk ◽  
Disease Risk ◽  
Protein Microarray ◽  
Specific Antigen ◽  
Principal Component ◽  
Health Concern ◽  
Limited Information ◽  
Antigen Specificity ◽  
E Coli ◽  
Geographical Regions

Breastfeeding provides defense against infectious disease during early life. The mechanisms underlying this protection are complex but likely include the vast array of immune cells and components, such as immunoglobulins, in milk. Simply characterizing the concentrations of these bioactives, however, provides only limited information regarding their potential relationships with disease risk in the recipient infant. Rather, understanding pathogen and antigen specificity profiles of milk-borne immunoglobulins might lead to a more complete understanding of how maternal immunity impacts infant health and wellbeing. Milk produced by women living in 11 geographically dispersed populations was applied to a protein microarray containing antigens from 16 pathogens, including diarrheagenic E. coli, Shigella spp., Salmonella enterica serovar Typhi, Staphylococcus aureus, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens of global health concern, and specific IgA and IgG binding was measured. Our analysis identified novel disease-specific antigen responses and suggests that some IgA and IgG responses vary substantially within and among populations. Patterns of antibody reactivity analyzed by principal component analysis and differential reactivity analysis were associated with either lower-to-middle-income countries (LMICs) or high-income countries (HICs). Antibody levels were generally higher in LMICs than HICs, particularly for Shigella and diarrheagenic E. coli antigens, although sets of S. aureus, S. pneumoniae, and some M. tuberculosis antigens were more reactive in HICs. Differential responses were typically specific to canonical immunodominant antigens, but a set of nondifferential but highly reactive antibodies were specific to antigens possibly universally recognized by antibodies in human milk. This approach provides a promising means to understand how breastfeeding and human milk protect (or do not protect) infants from environmentally relevant pathogens. Furthermore, this approach might lead to interventions to boost population-specific immunity in at-risk breastfeeding mothers and their infants.

scholarly journals Bacteriostasis of Escherichia coli by milk: IV. The bacteriostatic antibody of human milk

1979 ◽  
Vol 83 (2) ◽  
pp. 255-265 ◽  
Author(s):  
Jean M. Dolby ◽  
Pauline Honour
Keyword(s):  
Escherichia Coli ◽  
Human Milk ◽  
Bacteriostatic Activity ◽  
Gut Flora ◽  
Partial Loss ◽  
E Coli ◽  
Heat Labile ◽  
Resistant Strains

summaryBacteriostatic activity for milk-sensitive and milk-resistant strains of Escherichia coli is reduced when IgA is removed from milk by precipitation. Lysozyme is not involved in bacteriostasis and can be removed without loss of activity; heavy bentonite absorption however removes some lactoferrin causing partial loss of activity.The heat-labile antigen eliciting bacteriostatic antibody for E. coli is present in milk-sensitive and milk-resistant strains and in some other Enterobacteriaceae, e.g. salmonella; it cross reacts with the antigen in others, e.g. proteus and enterobacter. The antibody is therefore likely to be present in all human milk as a result of the normal commensal gut flora and with widespread activity.

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