Influence of pH and undissociated butyric acid on the production of acetone and butanol in batch cultures of Clostridium acetobutylicum

1984 ◽  
Vol 19 (6) ◽  
pp. 422-426 ◽  
Author(s):  
Fr�deric Monot ◽  
Jean-Marc Engasser ◽  
Henri Petitdemange
Keyword(s):  
Butyric Acid ◽  
Clostridium Acetobutylicum ◽  
Batch Cultures ◽  
Influence Of Ph

scholarly journals d-2,3-Butanediol Production Due to Heterologous Expression of an Acetoin Reductase in Clostridium acetobutylicum

2011 ◽  
Vol 77 (8) ◽  
pp. 2582-2588 ◽  
Author(s):  
Marco A. J. Siemerink ◽  
Wouter Kuit ◽  
Ana M. López Contreras ◽  
Gerrit Eggink ◽  
John van der Oost ◽  
...  
Keyword(s):  
Experimental Data ◽  
Heterologous Expression ◽  
Metabolic Network ◽  
Clostridium Acetobutylicum ◽  
Clostridium Beijerinckii ◽  
Racemic Mixture ◽  
Gene Encoding ◽  
Content Type ◽  
Batch Cultures

ABSTRACTAcetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions,Clostridium acetobutylicumATCC 824 (and strains derived from it) generates bothd- andl-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding ACR fromClostridium beijerinckiiNCIMB 8052 was functionally expressed inC. acetobutylicumunder the control of two strong promoters, the constitutivethlpromoter and the late exponentialadcpromoter. Both ACR-overproducing strains were grown in batch cultures, during which 89 to 90% of the natively produced acetoin was converted to 20 to 22 mMd-2,3-butanediol. The addition of a racemic mixture of acetoin led to the production of bothd-2,3-butanediol andmeso-2,3-butanediol. A metabolic network that is in agreement with the experimental data is proposed. Native 2,3-butanediol production is a first step toward a potential homofermentative 2-butanol-producing strain ofC. acetobutylicum.

scholarly journals Formic Acid Triggers the “Acid Crash” of Acetone-Butanol-Ethanol Fermentation byClostridium acetobutylicum

2011 ◽  
Vol 77 (5) ◽  
pp. 1674-1680 ◽  
Author(s):  
Shaohua Wang ◽  
Yanping Zhang ◽  
Hongjun Dong ◽  
Shaoming Mao ◽  
Yan Zhu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Formic Acid ◽  
Butyric Acid ◽  
Ethanol Fermentation ◽  
Clostridium Acetobutylicum ◽  
Recombinant Strain ◽  
Formate Dehydrogenase ◽  
Glucose Medium ◽  
Empty Plasmid ◽  
Acetone Butanol Ethanol

ABSTRACTSolvent production byClostridium acetobutylicumcollapses when cells are grown in pH-uncontrolled glucose medium, the so-called “acid crash” phenomenon. It is generally accepted that the fast accumulation of acetic acid and butyric acid triggers the acid crash. We found that addition of 1 mM formic acid into corn mash medium could trigger acid crash, suggesting that formic acid might be related to acid crash. When it was grown in pH-uncontrolled glucose medium or glucose-rich medium,C. acetobutylicumDSM 1731 containing the empty plasmid pIMP1 failed to produce solvents and was found to accumulate 0.5 to 1.24 mM formic acid intracellularly. In contrast, recombinant strain DSM 1731 with formate dehydrogenase activity did not accumulate formic acid intracellularly and could produce solvent as usual. We therefore conclude that the accumulation of formic acid, rather than acetic acid and butyric acid, is responsible for the acid crash of acetone-butanol-ethanol fermentation.

scholarly journals Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1

2018 ◽  
Vol 25 (2) ◽  
pp. 339-348 ◽  
Author(s):  
Najeeb Kaid Nasser Al-Shorgani ◽  
Mohd Sahaid Kalil ◽  
Wan Mohtar Wan Yusoff ◽  
Aidil Abdul Hamid
Keyword(s):  
Butyric Acid ◽  
Clostridium Acetobutylicum ◽  
Batch Fermentation ◽  
Butanol Production ◽  
Local Isolate

Metabolic engineering of Clostridium acetobutylicum for enhanced production of butyric acid

2013 ◽  
Vol 97 (21) ◽  
pp. 9355-9363 ◽  
Author(s):  
Yu-Sin Jang ◽  
Hee Moon Woo ◽  
Jung Ae Im ◽  
In Ho Kim ◽  
Sang Yup Lee
Keyword(s):  
Metabolic Engineering ◽  
Butyric Acid ◽  
Clostridium Acetobutylicum ◽  
Enhanced Production
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