The formation of ultrastructural epithelial and subepithelial connective tissue changes in experimentally induced cholesteatomas in guinea pigs

1985 ◽  
Vol 241 (3) ◽  
pp. 267-270 ◽  
Author(s):  
M. Galić ◽  
H. Heumann ◽  
D. Pohl ◽  
E. Steinbach
Keyword(s):  
Connective Tissue ◽  
Guinea Pigs ◽  
Tissue Changes ◽  
Experimentally Induced

THE HEXOSAMINE CONTENT OF THE ORBIT IN RELATION TO ANTI-THYROTROPHIN ANTIBODY TITRES IN SERA OF THYROTROPHIN-TREATED RABBITS WITH AND WITHOUT CORTISONE ADMINISTRATION

1962 ◽  
Vol 41 (3) ◽  
pp. 474-480 ◽  
Author(s):  
Otto Wegelius ◽  
E. J. Jokinen
Keyword(s):  
Connective Tissue ◽  
Guinea Pigs ◽  
Antibody Formation ◽  
Direct Proof ◽  
Significant Rise ◽  
Extraocular Muscles ◽  
Concomitant Treatment ◽  
Antibody Titres ◽  
Hexosamine Content ◽  
Synergetic Action

ABSTRACT In all previous investigations on experimental exophthalmos, heterologous thyrotrophic pituitary extracts have been used. These protein hormones stimulate antihormone formation in the test animals. Cortisone has been reported to effectively block antibody formation. In addition, it has been shown to potentiate TSH-induced exophthalmos in guinea-pigs. With rabbits as test animals, the hexosamine content of the orbital tissues was determined and used as an index of exophthalmos development and at the same time the antibody titres in the sera were followed. TSH injections for six weeks led to a highly significant accumulation of hexosamine in the retrobulbar connective tissue and in the extraocular muscles, i. e. an increase of up to 400% as compared with the control animals. At the same time a significant rise in antihormonal titres was detectable in the sera. Concomitant treatment with cortisone brought about an equal or higher accumulation of hexosamine but significantly lower antibody titres. The known opposite peripheral actions of TSH and cortisone can be reconciled with the synergy in producing experimental exophthalmos by attributing the synergetic action of cortisone to the blocking of antihormone formation. If less antihormones are produced, the effect of TSH is enhanced. Our experiments do not provide direct proof for this hypothesis. High hexosamine values in the orbit and low antihormone titres in the serum are, however, concomitant phenomena.

AN UNUSUAL CASE OF EXOPHTHALMOS WITH GENERAL CONNECTIVE TISSUE CHANGES

1960 ◽  
Vol XXXIII (IV) ◽  
pp. 613-622 ◽  
Author(s):  
B.-A. Lamberg ◽  
O. Wegelius ◽  
B. Kuhlbäck ◽  
C. Olin-Lamberg
Keyword(s):  
Renal Failure ◽  
Connective Tissue ◽  
Beneficial Effect ◽  
Muscle Tissue ◽  
Clinical Picture ◽  
Unusual Case ◽  
Systemic Treatment ◽  
Nasal Epithelium ◽  
Tissue Changes ◽  
Histochemical Examination

ABSTRACT A case is described of a man of 48, who presented a history and clinical picture of a solitary thyro-hypophysial syndrome with malignant exophthalmos but in which general connective tissue changes were found on histological and histochemical examination of the retrobulbar connective tissue and muscles, of the pretibial connective and muscle tissue and the nasal epithelium. The intraocular tension was increased. In addition, renal failure developed. The use of cortisol locally in the eyes had a beneficial effect on the eye syndrome. Systemic treatment with corticotrophin and prednisolone had an evident beneficial effect on the renal condition.

CONNECTIVE TISSUE CHANGES IN DIABETIC EYES

2009 ◽  
Vol 40 (3) ◽  
pp. 266-270 ◽  
Author(s):  
E. Linnér ◽  
B. Tengroth
Keyword(s):  
Connective Tissue ◽  
Tissue Changes

scholarly journals Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

2017 ◽  
Vol 6 (2) ◽  
pp. 1518
Author(s):  
Parvez Ikram ◽  
Nadeem Jeddy
Keyword(s):  
Connective Tissue ◽  
Large Scale ◽  
Oral Submucous Fibrosis ◽  
Muscle Layer ◽  
Inclusion Criteria ◽  
Polarizing Microscopy ◽  
Potentially Malignant ◽  
Tissue Changes ◽  
Submucous Fibrosis ◽  
Picrosirius Red

<p><strong>Background:</strong> Oral submucous fibrosis (OSMF), a potentially malignant oral disorder has the highest rate of malignant transformation of about 7-13%. The connective tissue changes that occur in this disease are characteristic and are stained with special stains.</p><p><strong>Objective:</strong> The study was done to compare common and special stains under light microscopy and polarizing microscopy to evaluate the levels of fibrosis in oral submucous fibrosis and assess the type of collagen present in the stromal area.</p><p><strong>Materials and Methods:</strong> Fifty tissue blocks were selected from the archives and were prepared and stained with H&amp;E, Masson's trichrome, Van Gieson and Picrosirius red and studied under light microscope and polarizing microscope respectively.</p><p><strong>Results:</strong> H and E stained slides were useful in diagnosing the lesion but was not able to highlight the level of fibrosis. Masson's trichrome and Van Gieson stained slides showed the depth of the lesion which extended even to the deeper muscle layer. The type of collagen present was definitively seen by the birefringence in polarizing microscopic study. Interobserver variation was less and all the values regarding the effectiveness of the special stains in detecting the level of fibrosis were statistically significant.</p><p><strong>Conclusion:</strong> Special stains can be used routinely in laboratories to demonstrate connective tissue lesions especially in cases of OSMF. Depth of the lesion and the area of involvement help in treatment planning to be delivered. Large scale studies with more categories and inclusion criteria are required along with the special stains to assess the other alterations in OSMF.</p>

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