The in vitro interactions of candida albicans with nonspecific serum proteins

1978 ◽  
Vol 65 (1-3) ◽  
pp. 169-176 ◽  
Author(s):  
H. F. Hasenclever
Keyword(s):  
Candida Albicans ◽  
Serum Proteins ◽  
In Vitro Interactions

scholarly journals In Vitro Interactions between Tacrolimus and Azoles against Candida albicans Determined by Different Methods

2007 ◽  
Vol 52 (2) ◽  
pp. 409-417 ◽  
Author(s):  
Shujuan Sun ◽  
Yan Li ◽  
Qiongjie Guo ◽  
Changwen Shi ◽  
Jinlong Yu ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Combination Therapy ◽  
Fungal Infections ◽  
Fungal Growth ◽  
Detection Methods ◽  
Positive Interactions ◽  
Data Generation ◽  
Nonparametric Models ◽  
In Vitro Interactions

ABSTRACT Combination therapy could be of use for the treatment of fungal infections, especially those caused by drug-resistant fungi. However, the methods and approaches used for data generation and result interpretation need further optimizing. The fractional inhibitory concentration index (FICI) is the most commonly used method, but it has several drawbacks in characterizing antifungal drug interaction. Alternatively, some new methods can be used such as the ΔE model (difference between the predicted and measured fungal growth percentages) and the response surface approach, which uses the concentration-effect relationship over the whole concentration range instead of just the MIC. In the present study, in vitro interactions between tacrolimus (FK506) and three azoles—fluconazole (FLC), itraconazole (ITR), and voriconazole (VRC)-against Candida albicans were evaluated by the checkerboard microdilution method and time-killing test. The intensity of the interactions was determined by visual reading and the spectrophotometric method in a checkerboard assay, and the nature of the interactions was assessed by nonparametric models of FICI and ΔE. Colony counting and colorimetric viable detection methods (2,3-bis {2-methoxy-4-nitro-5-[(sulfenylamino) carbonyl]-2H-tetrazolium hydroxide} [XTT] reduction test) were used for evaluating the combination antifungal effects over time. Synergistic and indifferent effects were found for the combination of FK506 and azoles against azole-sensitive strains, while strong synergy was found against azole-resistant strains analyzed by FICI. The ΔE model gave more consistent results with FICI. The positive interactions were also confirmed by the time-killing test. Our findings suggest a potential role for combination therapy with calcineurin pathway inhibitors and azoles to augment activity against resistant C. albicans.

In vitro interactions between Blastomyces dermatitidis and other zoopathogenic fungi

1988 ◽  
Vol 34 (7) ◽  
pp. 897-900 ◽  
Author(s):  
Vishnu P. Chaturvedi ◽  
Harbans S. Randhawa ◽  
Sudha Chaturvedi ◽  
Zia U. Khan
Keyword(s):  
Candida Albicans ◽  
Histoplasma Capsulatum ◽  
Sporothrix Schenckii ◽  
Blastomyces Dermatitidis ◽  
Microsporum Gypseum ◽  
Biotic Factors ◽  
Pseudallescheria Boydii ◽  
Natural Systems ◽  
In Vitro Interactions

The results of in vitro interactions between colonies of Blastomyces dermatitidis and six other zoopathogenic fungi are reported. The interactions were found to range from neutral with Histoplasma capsulatum and Candida albicans to strongly antagonistic with Microsporum gypseum, Pseudallescheria boydii, and Sporothrix schenckii, and including lysis by Cryptococcus neoformans. These observations suggest that interactions between zoopathogenic fungi may be one of the biotic factors likely to influence the occurrence of B. dermatitidis in natural systems.

In vitro interactions between azoles and amiodarone against clinical Candida albicans

2008 ◽  
Vol 31 (1) ◽  
pp. 88-90 ◽  
Author(s):  
Qiongjie Guo ◽  
Shujuan Sun ◽  
Yan Li ◽  
Jinlong Yu ◽  
Changwen Shi
Keyword(s):  
Candida Albicans ◽  
In Vitro Interactions

scholarly journals In vitro interactions between farnesol and fluconazole, amphotericin B or micafungin against Candida albicans biofilms

2014 ◽  
Vol 70 (2) ◽  
pp. 470-478 ◽  
Author(s):  
A. Katragkou ◽  
M. McCarthy ◽  
E. L. Alexander ◽  
C. Antachopoulos ◽  
J. Meletiadis ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Candida Albicans Biofilms ◽  
In Vitro Interactions

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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