Cellular reaction to Pneumocystis carinii

1973 ◽  
Vol 114 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Werner Dutz ◽  
Cornelius Post ◽  
Elfriede Kohout ◽  
A. Aghamohammadi
Keyword(s):  
Pneumocystis Carinii ◽  
Cellular Reaction

Immunogold labeling of Pneumocystis carinii for Electron microscopy

1991 ◽  
Vol 49 ◽  
pp. 270-271
Author(s):  
Michael P. Goheen ◽  
Marilyn S. Bartlett ◽  
James W. Smith
Keyword(s):  
Electron Microscopy ◽  
Pneumocystis Carinii ◽  
Severe Pneumonia ◽  
Culture Fluid ◽  
Immunogold Labeling ◽  
Antigenic Sites ◽  
Transmission Electron ◽  
Response To Chemotherapy ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

Studies of the biology of Pneumocystis carinii (PC) are of increasing importance because this extracellular pathogen is a frequent source of severe pneumonia in patients with acquired immunodeficiency syndrome (AIDS) and is a leading cause of mortality in these patients. Immunoelectron microscopic localization of antigenic sites on the surface of PC would improve the understanding of these sites and their role in pathenogenisis of the disease and response to chemotherapy. The purpose of this study was to develop a methodology for visualizing immunoreactive sites on PC with transmission electron microscopy (TEM) using immunogold labeled probes.Trophozoites of PC were added to spinner flask cultures and allowed to grow for 7 days, then aliquots of tissue culture fluid were centrifuged at 12,000 RPM for 30 sec. Pellets of organisims were fixed in either 1% glutaraldehyde, 0.1% glutaraldehyde-4% paraformaldehyde, or 4% paraformaldehyde for 4h. All fixatives were buffered with 0.1M Na cacodylate and the pH adjusted to 7.1. After fixation the pellets were rinsed in 0.1M Na cacodylate (3X), dehydrated with ethanol, and immersed in a 1:1 mixture of 95% ethanol and LR White resin.

Comparative morphology of Pneumocystis Carinii in Situ and after bronchoalveolar lavage

1986 ◽  
Vol 44 ◽  
pp. 244-245
Author(s):  
C. A. Itatani ◽  
A. Hing ◽  
W. Jackson ◽  
G.J. Marshall
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pneumocystis Carinii ◽  
Comparative Morphology

Pneumocystis carinii (PC) is an organism capable of causing fatal pneumonia in immune suppressed individuals and has recently gained prominence because of its association with AIDS. A similar organism occurs in rats and infection may be induced with cortisone injections. In order to isolate PC for further study bronchoal veol ar lavage (BAL) was performed. Differences in the ul trastructure of BAL-obtained organisms and PC in situ were observed and are herein reported.

Pneumocystis carinii-Pneumonie bei immunsupprimierten Patienten ohne AIDS-Erkrankung

Pneumologie ◽  
2005 ◽  
Vol 59 (S 1) ◽  
Author(s):  
T Blum ◽  
A Roth ◽  
H Mauch ◽  
H Lode
Keyword(s):  
Pneumocystis Carinii

Atypical Pneumocystis Carinii Pneumonia in a Child with Hyper-IgM Syndrome

1998 ◽  
Vol 18 (1) ◽  
pp. 71-78
Author(s):  
Michael Miller ◽  
Ilham Algayed ◽  
Ram Yogev ◽  
Pauline Chou ◽  
Paul Scholl ◽  
...  
Keyword(s):  
Pneumocystis Carinii Pneumonia ◽  
Pneumocystis Carinii ◽  
Hyper Igm Syndrome ◽  
Hyper Igm
Sign in / Sign up

Export Citation Format

Share Document