Determination of inorganic salts in acid and direct dyes by chromatography on polyamide

1977 ◽  
Vol 283 (5) ◽  
pp. 365-367 ◽  
Author(s):  
J. Gasparič ◽  
J. Pikhart
Keyword(s):  
Inorganic Salts ◽  
Direct Dyes

Determination of Inorganic Salts in Crude Oils

1942 ◽  
Vol 14 (12) ◽  
pp. 947-948 ◽  
Author(s):  
E. P. Rittershausen ◽  
R. J. DeGray
Keyword(s):  
Inorganic Salts ◽  
Crude Oils

THE DYEING OF NATURAL COTTON WITH DIRECT DYES: A DETERMINATION OF THE HEAT OF DYEING

1947 ◽  
Vol 25f (6) ◽  
pp. 303-315 ◽  
Author(s):  
R. P. Graham ◽  
H. J. Fromm
Keyword(s):  
Sodium Chloride ◽  
Water Solution ◽  
Cotton Yarn ◽  
Direct Dyes ◽  
Differential Heat ◽  
Equilibrium Sorption ◽  
Modern View ◽  
Direct Dye

The equilibrium sorption of a purified direct dye on natural cotton yarn has been determined over a range of dye-bath concentrations (20 to 175 mgm. of dye per litre of solution) and temperatures (75° to 90 °C.). The liquor-to-yarn ratio in the dye bath, which contained sodium chloride (4.00 gm. per litre), was sufficiently high to permit the concentration of dye in solution to remain essentially constant throughout a dyeing experiment. The dye was removed from the skeins using a pyridine–ethanol–water solution, and the concentration of dye in the latter determined spectrophotometrically.Differential heats of dyeing may be calculated from the results obtained. For an equilibrium sorption of 475 mgm. of Calcodur Blue 4GL per 100 gm. of natural cotton yarn, the value of 15 kgm-cal. per mole was found, and it is shown that the differential heat of dyeing increases as the value of the equilibrium sorption decreases. The results are interpreted in the light of the modern view of the structure of cellulose.

Serum Copper and Iron on a Single Sample

1958 ◽  
Vol 4 (1) ◽  
pp. 43-48 ◽  
Author(s):  
B Zak ◽  
N Ressler
Keyword(s):  
Quantitative Determination ◽  
Serum Sample ◽  
Organic Material ◽  
Serum Copper ◽  
Inorganic Salts ◽  
Organic Extract ◽  
Phenanthroline Complex ◽  
Extract Phase ◽  
Determination Of Copper

Abstract A procedure has been described for the quantitative determination of copper and iron in one serum sample. The sera are wet ashed to destroy organic material, leaving inorganic salts available in the residue for the subsequent spectrophotometric determination of iron as the ferrous-1,10-phenanthroline complex in the aqueous phase, and copper as the cuprous-2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline in an organic extract phase.

A 96-Well Plates-based UV Spectrophotometric Assay for Determination of Total Glucosinolates in Isatis indigotica Roots

2021 ◽  
Vol 17 ◽  
Author(s):  
Yanzhi Sun ◽  
Hongchao Zhang ◽  
Zhihong Cheng
Keyword(s):  
Quality Control ◽  
Enzymatic Hydrolysis ◽  
Quantitative Method ◽  
Rapid Determination ◽  
Spectrophotometric Assay ◽  
Inorganic Salts ◽  
Isatis Indigotica ◽  
Acceptable Accuracy ◽  
High Concentrations

Background: Glucosinolates (GLS) are important secondary metabolites in Cruciferae vegetables and herbs. Currently, the assays of total GLS determination are cumbersome (requiring acidic or enzymatic hydrolysis and addition of staining reagents), time-consuming, and indirect. High concentrations of inorganic salts are inevitably incorporated into the GLS products during separation. There is a need for a quantitative method for simple and rapid determination of total GLS after desalting process. Methods: A 96-well plates-based UV spectrophotometric method for determination of total GLS of Isatis indigotica roots was developed in the present study. The detection wavelength is set at 230 nm using quartz plates. This assay was validated using gluconapin and sinigrin as reference standards, and applied to determine the total GLS of I. indigotica roots prepared from five different desalting methods. Results: This assay is specific for total GLS prepared from I. indigotica roots, and it has acceptable accuracy (91.76–98.18% for quality control, and 95.59–102.52% for addition/recovery), precision (0.24–0.70% pooled RSD), reproducibility (0.31–1.84% RSD), and stability (0.24–1.45% RSD) over a 72-h period. Conclusion: The 96-well plates-based UV spectrophotometric assay is simple and accurate for high-throughput determination of total GLS.

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