Plasma glutathione peroxidase after selenium supplementation in patients with reduced selenium state

1982 ◽  
Vol 138 (2) ◽  
pp. 138-140 ◽  
Author(s):  
G. Steiner ◽  
H. Menzel ◽  
I. Lombeck ◽  
F. K. Ohnesorge ◽  
H. J. Bremer
Keyword(s):  
Glutathione Peroxidase ◽  
Selenium Supplementation ◽  
Plasma Glutathione Peroxidase ◽  
Plasma Glutathione

NUTRITIONAL SIGNIFICANCE OF SELENIUM SUPPLEMENTATION IN A SEMI-PURIFIED DIET FED DURING GESTATION AND LACTATION TO FIRST-LITTER GILTS AND THEIR PIGLETS

1985 ◽  
Vol 65 (2) ◽  
pp. 497-506 ◽  
Author(s):  
E. R. CHAVEZ
Keyword(s):  
Growth Rate ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Reproductive Performance ◽  
Mortality Rates ◽  
Selenium Supplementation ◽  
Plasma Glutathione Peroxidase ◽  
Litter Weight ◽  
Plasma Glutathione ◽  
Ad Libitum

Twelve purebred Landrace pregnant gilts were introduced to the experimental diets in pairs (littermates) in order to study the nutritional significance of 0.1 ppm Se supplementation (+Se) during gestation and lactation in a semi-purified diet containing adequate amounts of vitamin E but low in Se (0.018 ppm; −Se). Gilts were fed 2 kg/day during gestation and ad libitum during a 3-wk lactation. Animal response was evaluated in terms of reproductive performance, blood, colostrum and milk parameters. Litter size and total litter weight at birth and at weaning were not affected by the Se levels fed to the dams. Mortality rates at birth were 1.61 and 13.64% and from birth to weaning were 18.09 and 5.26% for +Se and −Se litters, respectively. A continuous decrease of blood Se content was observed in gilts throughout gestation, being significantly lower for the −Se group compared to the +Se group. Plasma glutathione peroxidase (GSH-Px) activity followed the same pattern observed in blood Se content. Content of Se in colostrum, although always higher than in milk, was significantly lower in the −Se gilts than the +Se group. Blood Se from piglets of the +Se gilts was significantly higher than the blood of piglets from litters of the −Se gilts at birth, and during the 3-wk lactation. Levels of GSH-Px activity in the plasma of piglets from litters of the two experimental groups of gilts were similar at birth, but by 3 wk lactation piglets from +Se gilts showed significantly greater GSH-Px activity than piglets from −Se gilts. Growth rate of the piglets at 4 wk postweaning was significantly affected by the dietary Se intake of their mothers during gestation and lactation. However, no differences in growth rate were observed among littermates that received a starter diet with or without Se supplementation. Key words: Selenium, gilts, pregnancy, lactation, piglets, postweaning

Mechanism of plasma glutathione peroxidase production in bovine adipocytes

2006 ◽  
Vol 326 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Tadashi Yamasaki ◽  
Koji Tahara ◽  
Shoichi Takano ◽  
Miho Inoue-Murayama ◽  
Michael T. Rose ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Plasma Glutathione Peroxidase ◽  
Plasma Glutathione ◽  
Bovine Adipocytes

scholarly journals Antihuman plasma glutathione peroxidase antibodies: immunologic investigations to determine plasma glutathione peroxidase protein and selenium content in plasma

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 318-323 ◽  
Author(s):  
N Avissar ◽  
JC Whitin ◽  
PZ Allen ◽  
IS Palmer ◽  
HJ Cohen
Keyword(s):  
Glutathione Peroxidase ◽  
Rat Plasma ◽  
Cross Reactivity ◽  
Healthy Individuals ◽  
Reactive Protein ◽  
Plasma Glutathione Peroxidase ◽  
Normal Plasma ◽  
Normal Individuals ◽  
Plasma Glutathione ◽  
Gshpx Activity

Abstract Plasma glutathione peroxidase (GSHPx) (glutathione: H2O2 oxidoreductase) is a unique selenoglycoprotein. Treatment of this enzyme with glycopeptidase F partially deglycosylates it and establishes the presence of N-linked sugar moieties. Antibodies raised in a rabbit against the purified enzyme from plasma were found to be specific, noninhibitory, and capable of precipitating the enzymatic activity. The antibodies precipitated greater than 90% of the GSHPx activity of normal plasma, thus indicating that the selenoenzyme is the main if not the sole GSHPx activity of plasma. The antibodies did not precipitate RBC GSHPx. A slight cross-reactivity of the antibodies was found with rat plasma GSHPx. A GSHPx activity precipitation assay of normal plasma in the presence of selenium (Se)-deficient plasma indicates that no cross-reactive protein in the Se-deficient plasma interferes with the precipitation of the GSHPx activity from normal plasma. Thus, GSHPx protein as well as activity is deficient in plasma in the absence of Se. Antibodies against GSHPx either from RBCs or from plasma were used to specifically immunoprecipitate most of the GSHPx activity from RBCs or plasma, respectively, in healthy individuals to determine the amount of Se associated with the protein. GSHPx accounts for approximately 15% of the Se in RBCs and 12% of the Se in plasma. Thus, in normal individuals, these proteins account for only a fraction of plasma and RBC Se.

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