Transformation to auxin-autotrophy and its reversibility in a mutant line of Crepis capillaris callus culture

1971 ◽  
Vol 110 (4) ◽  
pp. 355-360 ◽  
Author(s):  
M. D. Sacristán ◽  
M. F. Wendt-Gallitelli
Keyword(s):  
Callus Culture ◽  
Mutant Line ◽  
Crepis Capillaris ◽  
Auxin Autotrophy

Antioxidant and antihyperlipidemic activity of Tephrosia purpurea callus culture

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
M Mujeeb ◽  
M Aqil ◽  
AK Najmi ◽  
M Akhtar ◽  
N Ahmad ◽  
...  
Keyword(s):  
Callus Culture ◽  
Tephrosia Purpurea ◽  
Antihyperlipidemic Activity

Insecticidal Active Rotenoids from Plant Parts and Callus Culture of Medicago sativa L. from a Semiarid Region of India (Rajasthan)

2020 ◽  
Vol 16 (6) ◽  
pp. 937-941
Author(s):  
Sharad Vats ◽  
Preeti Mehra
Keyword(s):  
Medicago Sativa ◽  
Callus Culture ◽  
Point Of View ◽  
Semiarid Region ◽  
Disease Vectors ◽  
Vector Borne Diseases ◽  
Plant Parts ◽  
Resistant Varieties ◽  
Medicago Sativa L

Background: Vector-borne diseases are quite prevalent globally and are one of the major causes of deaths due to infectious diseases. There is an availability of synthetic insecticides, however, their excessive and indiscriminate use have resulted in the emergence of resistant varieties of insects. Thus, a search for novel biopesticide has become inevitable. Methods: Rotenoids were isolated and identified from different parts of Medicago sativa L. This group of metabolites was also identified in the callus culture, and the rotenoid content was monitored during subculturing for a period of 10 months. Enhancement of the rotenoid content was evaluated by feeding precursors in a tissue culture medium. Results: Four rotenoids (elliptone, deguelin, rotenone and Dehydrorotenone) were identified, which were confirmed using spectral and chromatographic techniques. The maximum rotenoid content was found in the seeds (0.33±0.01%), followed by roots (0.31±0.01%) and minimum in the aerial parts (0.20±0.05%). A gradual decrease in the rotenoid content was observed with the ageing of subcultured tissue maintained for 10 months. The production of rotenoids was enhanced up to 2 folds in the callus culture using amino acids, Phenylalanine and Methionine as precursors as compared to the control. The LC50 value of the rotenoids was found to be 91 ppm and 162 ppm against disease vectors of malaria and Dracunculiasis, respectively. Conclusion: The study projects M. sativa as a novel source of biopesticide against the disease vectors of malaria and Dracunculiasis. The use of precursors to enhance the rotenoid content in vitro can be an effective venture from a commercial point of view.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

Improving sesquiterpenoids production of Sarcandra glabra callus culture

2021 ◽  
Vol 169 ◽  
pp. 113636
Author(s):  
Li Li ◽  
Shan Li ◽  
Zhirong Cui ◽  
Yingying Wang ◽  
Yongyi Li ◽  
...  
Keyword(s):  
Callus Culture

Furofuran lignans from a callus culture of Cichorium intybus

2005 ◽  
Vol 24 (4) ◽  
pp. 246-249 ◽  
Author(s):  
J. Malarz ◽  
A. Stojakowska ◽  
E. Szneler ◽  
W. Kisiel
Keyword(s):  
Callus Culture ◽  
Cichorium Intybus
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