High incidence of Aspergillus flavus and aflatoxins in stored groundnut in Ghana and the use of a microbial assay to assess the inhibitory effects of plant extracts on aflatoxin synthesis

1996 ◽  
Vol 134 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Richard T. Awuah ◽  
Kafui A. Kpodo
1999 ◽  
Vol 62 (4) ◽  
pp. 414-417 ◽  
Author(s):  
J. J. FAN ◽  
J. H. CHEN

Welsh onion ethanol extracts were tested for their inhibitory activity against the growth and aflatoxin production of Aspergillus flavus and A. parasiticus. The survival of spores of A. flavus and A. parasiticus depended on both the extract concentration and the exposure time of the spores to the Welsh onion extracts. The mycelial growth of two tested fungi cultured on yeast extract–sucrose broth was completely inhibited in the presence of the Welsh onion ethanol extract at a concentration of 10 mg/ml during 30 days of incubation at 25°C. The extracts added to the cultures also inhibited aflatoxin production at a concentration of 10 mg/ml or permitted only a small amount of aflatoxin production with extract concentration of 5 mg/ml after 2 weeks of incubation. Welsh onion ethanol extracts showed more pronounced inhibitory effects against the two tested aflatoxin-producing fungi than did the same added levels of the preservatives sorbate and propionate at pH values near 6.5.


2021 ◽  
Vol 10 (2) ◽  
pp. 187
Author(s):  
Maliheh Soodi ◽  
Mohsen Shamsi ◽  
Homa Hajimehdipoor ◽  
Alireza Ghazanfari

Toxins ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 636 ◽  
Author(s):  
Zhao ◽  
Zhang ◽  
Folly ◽  
Chang ◽  
Wang ◽  
...  

Lactobacillus plantarum, as a natural bio-preservative, has attracted a great deal of attention in recent years. In this study, 22 L. plantarum strains were tested against the aflatoxin-producing fungus, Aspergillus flavus; strain IAMU80070 showed the highest antifungal activity. At a concentration of 5 × 105 colony-forming units (CFU) mL−1, it completely inhibited A. flavus growth and decreased aflatoxin production by 93%. Furthermore, ultrastructural examination showed that IAMU80070 destroyed the cellular structure of hyphae and spores. To explore the inhibitory effect of IAMU80070 on A. flavus at the transcriptional level, transcriptome data were obtained and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The aflatoxin biosynthetic process was the most significantly downregulated functional category, while genes implicated in the synthesis and organization of cell wall polysaccharides were upregulated. Quantitative real-time PCR results verified the credibility and reliability of the RNA sequencing data. These results provided insight into the transcriptome of A. flavus in response to the antagonistic effects of L. plantarum IAMU80070.


FLORESTA ◽  
2014 ◽  
Vol 45 (1) ◽  
pp. 163 ◽  
Author(s):  
José George Ferreira Medeiros ◽  
Aderson Costa Araujo Neto ◽  
Edcarlos Camilo Silva ◽  
Min Fu Nascimento Huang ◽  
Luciana Cordeiro Nascimento

As sementes são eficientes meios de disseminação e transmissão de patógenos e, frequentemente, introduzem novos focos de infecção em áreas isentas. A utilização de extratos de plantas com propriedades antimicrobianas são alternativas ecológicas e promissoras para substituir a proteção promovida pela aplicação de fungicidas. Assim, objetivou-se avaliar o efeito dos extratos de alamanda (Allamanda blanchetti L.) e melão-de-são-caetano (Momordica charantia L.) sobre a micoflora e a germinação de sementes de Caesalpinia ferrea. Para tanto, utilizaram-se lotes de sementes coletados em diferentes municípios paraibanos (Areia, Bananeiras, Conde e Remígio), os quais foram submetidos aos testes de sanidade e de germinação. Os tratamentos fitossanitários consistiram de Testemunha; Fungicida dicarboximida (240 g.100 kg-1) e extratos A. blanchetti e M. charantia nas concentrações de 10, 100, 500 e 1000 ppm. Constataram-se os fungos Aspergillus flavus, A. niger, Cladosporium sp., R. stolonifer, Penicillium sp. e Nigrospora sp. nas sementes de C. ferrea. Os extratos de A. blanchetti e M. charantia nas concentrações de 500 e 1000 ppm reduziram a incidência de fungos. As maiores concentrações (500 e 1000 ppm) de ambos os extratos promoveram o aumento no percentual e na velocidade de germinação (IVG), bem como no comprimento das plântulas de C. ferrea.Palavras-chave: Patologia de sementes; micoflora; controle alternativo; germinação. AbstractSanitary quality of Caesalpinia ferrea seeds: incidence of fungi, control and effects on physiological quality with the use of plant extracts. Seeds are efficient to dissemination and transmission of pathogens and often introduce new foci of infection in exempted areas. The use of plant extracts with antimicrobial properties are promising and environmentally friendly alternatives to replace the protection promoted by the application of fungicides. We aimed to evaluate the effect of extracts of Allamanda blanchetti L. and Momordica charantia L. on the mycoflora and seed germination of Caesalpinia ferrea. We used lots of seeds collected in different cities of Paraiba (Areia, Bananeiras, Conde e Remígio), which were tested for germination and sanity. The phytosanitary treatments consisted of control, dicarboximide fungicide (240 g.100 Kg-1) and extracts of A. blanchetti and M. charantia at concentrations of 10, 100, 500 and 1000 ppm. We identified in the seeds of C. ferrea fungi Aspergillus flavus, A. niger, Cladosporium sp., R. stolonifer, Penicillium sp. and Nigrospora sp. The extracts of A. blanchetti and M. charantia in concentrations of 500 and 1000 ppm reduced the incidence of fungi. The highest concentrations (500 and 1000 ppm) of both extracts increased percentage and germination rate (IVG) and length of seedlings of C. ferrea.Keywords: Pathology seed; mycoflora; alternative control; germination.


1992 ◽  
Vol 6 (5) ◽  
pp. 241-244 ◽  
Author(s):  
I. T. Kusumoto ◽  
I. Shimada ◽  
N. Kakiuchi ◽  
M. Hattori ◽  
T. Namba ◽  
...  

2012 ◽  
Vol 6 (41) ◽  
pp. 6931-6935 ◽  
Author(s):  
R M Abd El Aziz Abeer ◽  
R Al Othman Monira ◽  
A Al Sohaibani Saleh ◽  
A Mahmoud Mohamed ◽  
Murugan Kasi

1997 ◽  
Vol 20 (4) ◽  
pp. 313-317 ◽  
Author(s):  
Sung Yong Kim ◽  
Kun Ho Son ◽  
Hyun Wook Chang ◽  
Sam Sik Kang ◽  
Hyun Pyo Kim

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