Identification of the tRNA-binding sites on rat liver ribosomes by affinity labeling

1977 ◽  
Vol 153 (3) ◽  
pp. 231-235 ◽  
Author(s):  
A. Peter Czernilofsky ◽  
Ekkehard Collatz ◽  
Axel M. Gressner ◽  
Ira G. Wool ◽  
Ernst Küchler
Keyword(s):  
Rat Liver ◽  
Binding Sites ◽  
Affinity Labeling ◽  
Trna Binding ◽  
Liver Ribosomes

Identification of proteins at the peptidyl-tRNA binding site of rat liver ribosomes

1981 ◽  
Vol 184 (3) ◽  
pp. 551-556 ◽  
Author(s):  
Steven Fabijanski ◽  
Maria Pellegrini
Keyword(s):  
Binding Site ◽  
Rat Liver ◽  
Trna Binding ◽  
Liver Ribosomes

Three tRNA Binding Sites in Rabbit Liver Ribosomes and Role of the Intrinsic ATPase in 80S Ribosomes from Higher Eukaryotes†

Biochemistry ◽  
1997 ◽  
Vol 36 (34) ◽  
pp. 10492-10497 ◽  
Author(s):  
Anna V. El'skaya ◽  
Galina V. Ovcharenko ◽  
Sergey S. Palchevskii ◽  
Zoja M. Petrushenko ◽  
Francisco J. Triana-Alonso ◽  
...  
Keyword(s):  
Binding Sites ◽  
Rabbit Liver ◽  
Higher Eukaryotes ◽  
Trna Binding ◽  
Liver Ribosomes

[25] Affinity labeling of tRNA-binding sites on ribosomes

1988 ◽  
pp. 372-397 ◽  
Author(s):  
James Ofengand ◽  
Robert Denman ◽  
Kelvin Nurse ◽  
Arnold Liebman ◽  
David Malarek ◽  
...  
Keyword(s):  
Binding Sites ◽  
Affinity Labeling ◽  
Trna Binding

m-[o-(2-Chloro-5-Fluorosulfonylphenylureido)Phenoxybutoxy]Benzamidine: Affinity Labeling Reagent Which Can Identify Secondary Binding Sites of Coagulation Complement and Fibrinolytic Serine Proteases

1979 ◽  
Author(s):  
D Bing ◽  
D Robison ◽  
J Andrews ◽  
R Laura
Keyword(s):  
Binding Sites ◽  
Serine Proteases ◽  
Enzyme Inhibitor ◽  
Molecular Model ◽  
Factor Xa ◽  
Affinity Labeling ◽  
Catalytic Center ◽  
Inhibitor Complex ◽  
Polypeptide Chains ◽  
Kinetics Of

We have determined that m-[o-(2-chloro-5-fluorosulfonylphenylureido)phenoxybutoxy]benza-midine [mCP(PBA)-F] is an affinity labeling reagent which labels both polypeptide chains of thrombin, factor Xa, complement component CIS and plasmin. As this means it is reacting outside of the catalytic center, we have called this reagent an exo-site affinity labeling reagent. Progressive irreversible inhibition of these enzymes by this reagent is rapid (k1st 2.5-4.6 x 10-3sec-1), the kinetics of inactivation are consistent with inhibition proceding via formation of a specific enzyme-inhibitor complex analogous to a Michaelis-Menton complex (KL - 115-26 μM), and diisopropylfluorophosphate or p-amidino-phenylmethanesulfonyfluoride Prevent labeling by [3H]mCP(PBA)-F. A molecular model of mCP(PBA)-F shows that the reactive SO2F group can be 17 A from the cationic amidine. The data are consistent with the hypothesis that both peptide chains are required for the specific proteolytic activity exhibited by these proteases and that the peptide chain which does not contain the active site serine is close to the catalytic center. (Supported by NIH and AHA grants

scholarly journals Binding of Purified Antialbumin by Rat Liver Ribosomes

1965 ◽  
Vol 240 (7) ◽  
pp. 3009-3015 ◽  
Author(s):  
William A. Warren ◽  
Theodore Peters
Keyword(s):  
Rat Liver ◽  
Liver Ribosomes

scholarly journals tRNA binding stabilizes rat liver 60 S ribosomal subunits during treatment with LiCl.

1980 ◽  
Vol 255 (14) ◽  
pp. 6954-6961 ◽  
Author(s):  
A.M. Reboud ◽  
S. Dubost ◽  
M. Buisson ◽  
J.P. Reboud
Keyword(s):  
Rat Liver ◽  
Ribosomal Subunits ◽  
Trna Binding
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