Structure determination of organic compounds by ?electron pyrolysis?

1977 ◽  
Vol 286 (5) ◽  
pp. 355-360 ◽  
Author(s):  
G. Ege ◽  
K. Ge�ner ◽  
H. Schildknecht
2020 ◽  
Author(s):  
Ernö Pretsch ◽  
Philippe Bühlmann ◽  
Martin Badertscher

Author(s):  
Ernö Pretsch ◽  
Thomas Clerc ◽  
Joseph Seibl ◽  
Wilhelm Simon

1967 ◽  
Vol 16 (3) ◽  
pp. 210-216 ◽  
Author(s):  
Masahiko TSUCHIYA ◽  
Shinya MATSUHIRA ◽  
Satoshi ISHIDO

Author(s):  
Ernö Pretsch ◽  
Thomas Clerc ◽  
Joseph Seibl ◽  
Wilhelm Simon

2019 ◽  
Author(s):  
Heng zhou ◽  
Feng Luo ◽  
Zhipu Luo ◽  
Dan Li ◽  
Cong Liu ◽  
...  

AbstractMicrocrystal electron diffraction (MicroED) is becoming a powerful tool in determining the crystal structures of biological macromolecules and small organic compounds. However, wide applications of this technique are still limited by the special requirement for radiation-tolerated movie-mode camera and the lacking of automated data collection method. Herein, we develop a stage-camera synchronization scheme to minimize the hardware requirements and enable the use of the conventional electron cryo-microscope with single-frame CCD camera, which ensures not only the acquisition of ultrahigh-resolution diffraction data but also low cost in practice. This method renders the structure determination of both peptide and small organic compounds at ultrahigh resolution up to ~0.60 Å with unambiguous assignment of nearly all hydrogen atoms. The present work provides a widely applicable solution for routine structure determination of MicroED, and demonstrates the capability of the low-end 120kV microscope with a CCD camera in solving ultra-high resolution structures of both organic compound and biological macromolecules.


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