A general method for isolation of Mu d 1–8(Apr lac) operon fusions in Salmonella typhimurium LT2 from Tn10 insertion strains: chlC::Mu d 1–8

1986 ◽  
Vol 205 (2) ◽  
pp. 221-224
Author(s):  
H. S. Kwan ◽  
K. K. Wong
Keyword(s):  
Salmonella Typhimurium ◽  
Lac Operon ◽  
Tn10 Insertion ◽  
General Method

scholarly journals Characterization of lip expression in Salmonella typhimurium: analysis of lip::lac operon fusions

1991 ◽  
Vol 137 (10) ◽  
pp. 2307-2312 ◽  
Author(s):  
R. L. Smith ◽  
J. W. Pelley ◽  
R. M. Jeter
Keyword(s):  
Salmonella Typhimurium ◽  
Lac Operon

scholarly journals Oxygen-regulated stimulons of Salmonella typhimurium identified by Mu d(Ap lac) operon fusions.

1986 ◽  
Vol 165 (3) ◽  
pp. 780-786 ◽  
Author(s):  
Z Aliabadi ◽  
F Warren ◽  
S Mya ◽  
J W Foster
Keyword(s):  
Salmonella Typhimurium ◽  
Lac Operon

scholarly journals FUNCTIONAL INTERCHANGEABILITY OF DNA REPLICATION GENES IN SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI DEMONSTRATED BY A GENERAL COMPLEMENTATION PROCEDURE

Genetics ◽  
1984 ◽  
Vol 108 (1) ◽  
pp. 1-23 ◽  
Author(s):  
Russell Maurer ◽  
Barbara C Osmond ◽  
Eugene Shekhtman ◽  
Annette Wong ◽  
David Botstein
Keyword(s):  
Dna Replication ◽  
Salmonella Typhimurium ◽  
Genomic Library ◽  
Selectable Marker ◽  
Temperature Sensitive ◽  
Replication Proteins ◽  
E Coli ◽  
Temperature Sensitive Mutants ◽  
Chromosomal Mutations ◽  
General Method

ABSTRACT Twenty-four genes from Salmonella typhimurium that affect DNA replication were isolated from a λ-Salmonella genomic library by lysogenic complementation of temperature-sensitive mutants of Salmonella or E. coli, using a new plaque complementation assay. The complementing λ clones, which make red plaques in this assay, and noncomplementing mutant derivatives, which make uncolored plaques, were used to further characterize the temperature-sensitive Salmonella mutants and to establish the functional similarity of E. coli and Salmonella DNA replication genes. For 17 of 18 E. coli mutants representing distinct loci, a Salmonella gene that complemented the mutant was found. This result indicates that single Salmonella replication proteins are able to function in otherwise all E. coli replication complexes and suggests that the detailed properties of Salmonella and E. coli replication proteins are very similar. The other seven Salmonella genes that were cloned were unrelated functionally to any E. coli genes examined. —As an aid to the derivation of chromosomal mutations affecting some of the cloned genes, a general method was developed for placing a transposon in the Salmonella chromosome in a segment corresponding to cloned DNA. Chromosomal mutations were derived in Salmonella affecting a gene (dnaA) that was cloned by complementation of an E. coli mutant by using the transposon-encoded drug resistance as a selectable marker in local mutagenesis.

Isolation and characterization of pyrimidine mutants of Salmonella typhimurium altered in expression of pyrC, pyrD, and pyrE

1985 ◽  
Vol 31 (11) ◽  
pp. 981-987 ◽  
Author(s):  
Rod A. Kelln ◽  
J. Neuhard ◽  
Lisbeth Stauning
Keyword(s):  
Salmonella Typhimurium ◽  
Biochemical Characterization ◽  
Lac Operon ◽  
Structural Genes ◽  
General Applicability ◽  
Cis Acting ◽  
Isolation And Characterization ◽  
Mutant Strains ◽  
The Individual

Parental strains of Salmonella typhimurium having a specific pyr gene (pyrC, pyrD, or pyrE) fused to the structural genes of the lac operon through the specialized transducing phage Mu dl (ApRlac) were used to construct thermostable derivatives for purposes of conducting a genetic and biochemical characterization of the individual pyr genes. The direction of transcription of each pyr gene in relation to the current linkage map was defined with both pyrC and pyrE being transcribed counterclockwise and pyrD exhibiting clockwise transcription. Mutants displaying increased pyr gene expression were isolated employing a genetic strategy which is of general applicability. Among the mutants, only one isolate was found to possess a mutation which was unlinked to the specific pyr gene under study; the other isolates harbored linked mutations which were inferred to be cis-acting. Additional studies demonstrated that in conditions of severe pyrimidine limitation, further derepression could still occur in the mutant strains.

ack::Mu d1-8 (Apr lac) operon fusions of Salmonella typhimurium LT2

1988 ◽  
Vol 211 (1) ◽  
pp. 183-185 ◽  
Author(s):  
H. S. Kwan ◽  
H. W. Chui ◽  
K. K. Wong
Keyword(s):  
Salmonella Typhimurium ◽  
Lac Operon

A General Method for Spectrometer Design in the Scoff Approximation

1976 ◽  
Vol 34 ◽  
pp. 538-539
Author(s):  
J. R. Fields
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Energy Analysis ◽  
Incident Beam ◽  
Scanning Transmission Electron Microscope ◽  
Chemical Identification ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
General Method

The energy analysis of electrons scattered by a specimen in a scanning transmission electron microscope can improve contrast as well as aid in chemical identification. In so far as energy analysis is useful, one would like to be able to design a spectrometer which is tailored to his particular needs. In our own case, we require a spectrometer which will accept a parallel incident beam and which will focus the electrons in both the median and perpendicular planes. In addition, since we intend to follow the spectrometer by a detector array rather than a single energy selecting slit, we need as great a dispersion as possible. Therefore, we would like to follow our spectrometer by a magnifying lens. Consequently, the line along which electrons of varying energy are dispersed must be normal to the direction of the central ray at the spectrometer exit.

Phase behavior of cationic and anionic surfactants at low concentrations

1992 ◽  
Vol 50 (1) ◽  
pp. 694-695
Author(s):  
E. Naranjo
Keyword(s):  
Phase Behavior ◽  
Structural Characterization ◽  
Dynamic Systems ◽  
Anionic Surfactants ◽  
Intermolecular Forces ◽  
Stable Form ◽  
Surfactant Mixtures ◽  
Low Concentrations ◽  
Cationic And Anionic Surfactants ◽  
General Method

Equilibrium vesicles, those which are the stable form of aggregation and form spontaneously on mixing surfactant with water, have never been demonstrated in single component bilayers and only rarely in lipid or surfactant mixtures. Designing a simple and general method for producing spontaneous and stable vesicles depends on a better understanding of the thermodynamics of aggregation, the interplay of intermolecular forces in surfactants, and an efficient way of doing structural characterization in dynamic systems.

Caricaturing as a general method to improve poor face recognition: Evidence from low-resolution images, other-race faces, and older adults.

2019 ◽  
Vol 25 (2) ◽  
pp. 256-279 ◽  
Author(s):  
Amy Dawel ◽  
Tsz Ying Wong ◽  
Jodie McMorrow ◽  
Callin Ivanovici ◽  
Xuming He ◽  
...  
Keyword(s):  
Older Adults ◽  
Face Recognition ◽  
Low Resolution ◽  
General Method ◽  
Low Resolution Images
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