Bullous pemphigoid antigen concentration in normal human skin in relation to body area and age

1988 ◽  
Vol 280 (7) ◽  
pp. 416-419 ◽  
Author(s):  
G. Hamm ◽  
K. -D. Wozniak
Keyword(s):  
Human Skin ◽  
Bullous Pemphigoid ◽  
Body Area ◽  
Antigen Concentration ◽  
Normal Human Skin ◽  
Bullous Pemphigoid Antigen ◽  
Normal Human

scholarly journals Degradation of the epidermal-dermal junction by proteolytic enzymes from human skin and human polymorphonuclear leukocytes.

1984 ◽  
Vol 160 (4) ◽  
pp. 1027-1042 ◽  
Author(s):  
R A Briggaman ◽  
N M Schechter ◽  
J Fraki ◽  
G S Lazarus
Keyword(s):  
Human Skin ◽  
Bullous Pemphigoid ◽  
Polymorphonuclear Leukocytes ◽  
Skin Diseases ◽  
Proteolytic Enzymes ◽  
Cathepsin G ◽  
Basal Cells ◽  
Lamina Densa ◽  
Normal Human Skin ◽  
Bullous Pemphigoid Antigen

The degradation of normal human skin by the human polymorphonuclear leukocyte proteinases cathepsin G and elastase, and by a human skin chymotrypsin-like proteinase that appears to be a mast cell constituent, was examined. Enzymes were incubated with fresh, split-thickness skin for up to 8 h; the tissue was examined ultrastructurally and immunohistochemically using antibodies to known basement membrane constituents. In all cases, the primary damage observed was at the epidermal-dermal junction. Elastase degraded the lamina densa leaving scattered and disorganized anchoring fibrils, dermal microfibril bundles, and normal-appearing collagen fibers. Immunohistochemically, type IV collagen, laminin, KF1 antigen, and EBA antigen were absent. The bullous pemphigoid antigen was present and localized on the basal cells. Epidermal-dermal separation produced by the chymotrypsin-like proteinases, cathepsin G, and the human skin proteinase, was confined to the lamina lucida. The lamina densa and sub-lamina densa fibrillar network remained intact. The human skin chymotrypsin-like proteinase produced extensive epidermal-dermal separation, while cathepsin G, at comparable concentrations, produced only focal separations. Immunohistochemically, all antigens were present after incubation with enzyme. The bullous pemphigoid antigen, however, was found on the epidermal side of the split, while laminin was found on the dermal side. These results show that the epidermal-dermal junction is highly susceptible to neutral serine proteinases located in mast cells and polymorphonuclear leukocytes. Although all the proteinases produce epidermal-dermal separation, the patterns and extent of degradation are different. The distinctive patterns of degradation may provide a clue to the involvement of these proteinases in skin diseases.

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

Type I and III collagen content and fibre distribution in normal human skin during ageing

1987 ◽  
Vol 117 (4) ◽  
pp. 419-428 ◽  
Author(s):  
C.R. LOVELL ◽  
K.A. SMOLENSKI ◽  
V.C. DUANCE ◽  
N.D. LIGHT ◽  
S. YOUNG ◽  
...  
Keyword(s):  
Human Skin ◽  
Collagen Content ◽  
Type I ◽  
Normal Human Skin ◽  
Fibre Distribution ◽  
Normal Human

scholarly journals C3b Receptor in Normal Human Skin

1981 ◽  
Vol 77 (4) ◽  
pp. 353-357 ◽  
Author(s):  
Hakan N. Thyresson ◽  
Frederic C. McDuffie ◽  
Arnold L. Schroeter
Keyword(s):  
Human Skin ◽  
Normal Human Skin ◽  
C3b Receptor ◽  
Normal Human
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