Optimum DNA relaxation reaction conditions for calf thymus DNA-Topoisomerase I are determined by specific enzyme features

1990 ◽  
Vol 14 (4) ◽  
pp. 255-259 ◽  
Author(s):  
S. Coderoni ◽  
M. Paparelli ◽  
G. L. Gianfranceschi
Keyword(s):  
Topoisomerase I ◽  
Calf Thymus Dna ◽  
Dna Topoisomerase I ◽  
Specific Enzyme ◽  
Reaction Conditions ◽  
Dna Topoisomerase ◽  
Dna Relaxation ◽  
Calf Thymus

2′,5′-01igoadenylates Inhibit Relaxation of Supercoiled DNA by Calf Thymus DNA Topoisomerase I

1991 ◽  
Vol 11 (3) ◽  
pp. 143-149 ◽  
Author(s):  
FRANK J. CASTORA ◽  
CATHERINE E. ERICKSON ◽  
TERÉZ KOVÁCS ◽  
KRYSTYNA LESIAK ◽  
PAUL F. TORRENCE
Keyword(s):  
Topoisomerase I ◽  
Calf Thymus Dna ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Supercoiled Dna ◽  
Calf Thymus

scholarly journals Inhibition of Escherichia coli DNA topoisomerase I activity by phospholipids

1992 ◽  
Vol 285 (2) ◽  
pp. 503-506 ◽  
Author(s):  
T Mizushima ◽  
S Natori ◽  
K Sekimizu
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Topoisomerase I ◽  
Saturated Fatty Acids ◽  
Egg Yolk ◽  
Inhibitory Effect ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
E Coli ◽  
Dna Relaxation

The DNA relaxation activity of Escherichia coli DNA topoisomerase I in vitro was greatly inhibited by cardiolipin. Inhibition also occurred to some extent with phosphatidylglycerol from egg yolk. Analysis with synthetic phospholipid revealed that phosphatidylglycerol containing unsaturated fatty acids exhibited a strong inhibitory effect, whereas inhibition by phosphatidylglycerol containing saturated fatty acids was weak. Phosphatidylethanolamine showed no inhibitory effect. Chlorpromazine, which interacts with phospholipids, suppressed the inhibitory effect of cardiolipin. Cardiolipin and phosphatidylglycerol with unsaturated fatty acid precipitated topoisomerase I even at low concentrations, whereas phosphatidylglycerol from egg yolk and a synthetic phosphatidylglycerol containing saturated fatty acids precipitated this enzyme only at high concentrations. One-third of the total topoisomerase I in E. coli was found in the membrane fraction. Treatment of E. coli cells with chlorpromazine resulted in relaxation of plasmid DNA. This DNA relaxation was not observed in a topA mutant, suggesting that this relaxation by chlorpromazine in vivo is catalysed by topoisomerase I.

scholarly journals DNA topoisomerase I from calf thymus is inhibited in vitro by poly(ADP-ribosylation)

1983 ◽  
Vol 136 (2) ◽  
pp. 391-396 ◽  
Author(s):  
Jenny JONGSTRA-BILEN ◽  
Marie-Elisabeth ITTEL ◽  
Claude NIEDERGANG ◽  
Hans-Peter VOSBERG ◽  
Paul MANDEL
Keyword(s):  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Adp Ribosylation ◽  
Calf Thymus
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