Treponema bryantii sp. nov., a rumen spirochete that interacts with cellulolytic bacteria

1980 ◽  
Vol 127 (2) ◽  
pp. 145-156 ◽  
Author(s):  
T. B. Stanton ◽  
E. Canale-Parola
Keyword(s):  
Cellulolytic Bacteria ◽  
Treponema Bryantii

Interactions between Treponema bryantii and cellulolytic bacteria in the in vitro degradation of straw cellulose

1987 ◽  
Vol 33 (3) ◽  
pp. 244-248 ◽  
Author(s):  
H. Kudo ◽  
K.-J. Cheng ◽  
J. W. Costerton
Keyword(s):  
Plant Cell Wall ◽  
Bacterial Species ◽  
Barley Straw ◽  
Cellulolytic Bacteria ◽  
Morphological Examination ◽  
Cellulose Digestion ◽  
Acid Generation ◽  
Pure Cultures ◽  
Treponema Bryantii

To assess the contribution of individual bacterial species to the overall process of cellulose digestion in the rumen, cellulolytic bacteria (Bacteroides succinogenes and Ruminococcus albus) were tested as pure cultures and as cocultures with noncellulolytic Treponema bryantii. In studies of in vitro barley straw digestion, Treponema cocultures surpassed pure cultures of the cellulolytic organisms in dry matter disappearance, volatile fatty acid generation, and in the production of succinic acid, lactic acid, and ethanol. Morphological examination, by electron microscopy, showed that cells of T. bryantii associate with the plant cell wall materials in straw, but that cellulose digestion occurs only when these organisms are present with cellulolytic species such as B. succinogenes. These results show that cellulolytic bacteria interact with noncellulolytic Treponema to promote the digestion of cellulosic materials.

scholarly journals Changes in the Rumen Bacteriome Structure and Enzymatic Activities of Goats in Response to Dietary Supplementation with Schizochytrium spp.

2021 ◽  
Vol 9 (7) ◽  
pp. 1528
Author(s):  
Alexandros Mavrommatis ◽  
Dimitrios Skliros ◽  
Emmanouil Flemetakis ◽  
Eleni Tsiplakou
Keyword(s):  
Dairy Products ◽  
Rumen Fluid ◽  
Cellulase Activity ◽  
Control Group ◽  
Cellulolytic Bacteria ◽  
Sequencing Platform ◽  
Rumen Microbiome ◽  
Nutritional Strategy ◽  
C 50 ◽  
Treponema Bryantii

With the aim to produce functional dairy products enriched with polyunsaturated fatty acids (PUFA) by using feed supplements, radical changes could occur in the rumen microbiome. This work investigated the alterations of the rumen bacteriome of goats fed with PUFA-rich marine microalgae Schizochytrium spp. For the trial, twenty-four goats were divided into four homogenous clusters (six goats/treatment) according to their fat-corrected (4%) milk yield, body weight, and age; they were individually fed with alfalfa hay and a concentrate (F/C = 50/50). The concentrate of the control group (CON) contained no microalgae, while those of the treated groups were supplemented daily with 20 (ALG20), 40 (ALG40), and 60 g (ALG60) of Schizochytrium spp./goat. Rumen fluid samples were collected using a stomach tube during the 20th and 40th days of the experiment. The microbiome analysis using a 16S rRNA sequencing platform revealed that Firmicutes were decreased in microalgae-fed goats, while Bacteroidetes showed a tendency to increase in the ALG40 group due to the enhancement of Prevotellaceae. Cellulolytic bacteria, namely Treponema bryantii, Ruminococcus gauvreauii, R. albus, and R. flavefaciens, were decreased in the ALG40 group, resulting in an overall decrease of cellulase activity. In contrast, the amylolytic potential was significantly enhanced due to an upsurge in Ruminobacter amylophilus, Succinivibrio dextrinosolvens, and Fretibacterium fastidiosum populations. In conclusion, supplementing goats’ diets with 20 g Schizochytrium spp. could be considered a sustainable and efficient nutritional strategy to modulate rumen microbiome towards the development of dairy products enriched with bioactive compounds, while higher levels induced substantial shifts in determinant microbes’ populations.

Exploring the functional significance of novel cellulolytic bacteria for the anaerobic digestion of rice straw

2021 ◽  
Vol 169 ◽  
pp. 485-497
Author(s):  
Vikas Kumar ◽  
Jyoti Rawat ◽  
Ravichandra C. Patil ◽  
Chitta Ranjan Barik ◽  
Sukumar Purohit ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Rice Straw ◽  
Functional Significance ◽  
Cellulolytic Bacteria

scholarly journals Effects of Methanogenic Inhibitors on Methane Production and Abundances of Methanogens and Cellulolytic Bacteria inIn VitroRuminal Cultures

2011 ◽  
Vol 77 (8) ◽  
pp. 2634-2639 ◽  
Author(s):  
Zhenming Zhou ◽  
Qingxiang Meng ◽  
Zhongtang Yu
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Cellulolytic Bacteria ◽  
Bacterial Populations ◽  
Content Type ◽  
Specific Pcr ◽  
Methanogen Population ◽  
Propynoic Acid

ABSTRACTThe objective of this study was to systematically evaluate and compare the effects of select antimethanogen compounds on methane production, feed digestion and fermentation, and populations of ruminal bacteria and methanogens usingin vitrocultures. Seven compounds, including 2-bromoethanesulphonate (BES), propynoic acid (PA), nitroethane (NE), ethyltrans-2-butenoate (ETB), 2-nitroethanol (2NEOH), sodium nitrate (SN), and ethyl-2-butynote (EB), were tested at a final concentration of 12 mM. Ground alfalfa hay was included as the only substrate to simulate daily forage intake. Compared to no-inhibitor controls, PA, 2NEOH, and SN greatly reduced the production of methane (70 to 99%), volatile fatty acids (VFAs; 46 to 66%), acetate (30 to 60%), and propionate (79 to 82%), with 2NEOH reducing the most. EB reduced methane production by 23% without a significant effect on total VFAs, acetate, or propionate. BES significantly reduced the propionate concentration but not the production of methane, total VFAs, or acetate. ETB or NE had no significant effect on any of the above-mentioned measurements. Specific quantitative-PCR (qPCR) assays showed that none of the inhibitors significantly affected total bacterial populations but that they did reduce theFibrobacter succinogenespopulation. SN reduced theRuminococcus albuspopulation, while PA and 2NEOH increased the populations of bothR. albusandRuminococcus flavefaciens. Archaeon-specific PCR-denaturing gradient gel electrophoresis (DGGE) showed that all the inhibitors affected the methanogen population structure, while archaeon-specific qPCR revealed a significant decrease in methanogen population in all treatments. These results showed that EB, ETB, NE, and BES can effectively reduce the total population of methanogens but that they reduce methane production to a lesser extent. The results may guide futureinvivostudies to develop effective mitigation of methane emission from ruminants.

Cellulolytic bacteria from soils in harsh environments

2012 ◽  
Vol 28 (5) ◽  
pp. 2195-2203 ◽  
Author(s):  
Fábio Lino Soares ◽  
Itamar Soares Melo ◽  
Armando Cavalcante Franco Dias ◽  
Fernando Dini Andreote
Keyword(s):  
Harsh Environments ◽  
Cellulolytic Bacteria

Diarrhea-associated pathogens, lactobacilli and cellulolytic bacteria in equine feces: Responses to antibiotic challenge

2013 ◽  
Vol 166 (1-2) ◽  
pp. 225-232 ◽  
Author(s):  
Brittany E. Harlow ◽  
Laurie M. Lawrence ◽  
Michael D. Flythe
Keyword(s):  
Cellulolytic Bacteria

Influence of an aerobic fungus grown on solid culture on ruminal degradability and on a mixture culture of anaerobic cellulolytic bacteria

2009 ◽  
Vol 94 (3) ◽  
pp. 330-337
Author(s):  
R Hernández-Díaz ◽  
D. J Pimentel-González ◽  
A. C Figueira ◽  
G Viniegra-González ◽  
R. G Campos-Montiel
Keyword(s):  
Cellulolytic Bacteria ◽  
Solid Culture ◽  
Ruminal Degradability
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