Isolation of the RAD18 gene of Saccharomyces cerevisiae and construction of rad18 deletion mutants

1989 ◽  
Vol 215 (3) ◽  
pp. 425-430 ◽  
Author(s):  
Francis Fabre ◽  
Nieve Magana-Schwencke ◽  
Roland Chanet
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Deletion Mutants

scholarly journals A Genome-Wide Screen in Saccharomyces cerevisiae Reveals a Critical Role for Oxidative Phosphorylation in Cellular Tolerance to Lithium Hexafluorophosphate

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 888
Author(s):  
Xuejiao Jin ◽  
Jie Zhang ◽  
Tingting An ◽  
Huihui Zhao ◽  
Wenhao Fu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oxidative Phosphorylation ◽  
Cellular Response ◽  
Critical Role ◽  
Lithium Ion ◽  
Deletion Mutants ◽  
High Concentration ◽  
Genome Wide ◽  
A Genome ◽  
Lithium Hexafluorophosphate

Lithium hexafluorophosphate (LiPF6) is one of the leading electrolytes in lithium-ion batteries, and its usage has increased tremendously in the past few years. Little is known, however, about its potential environmental and biological impacts. In order to improve our understanding of the cytotoxicity of LiPF6 and the specific cellular response mechanisms to it, we performed a genome-wide screen using a yeast (Saccharomyces cerevisiae) deletion mutant collection and identified 75 gene deletion mutants that showed LiPF6 sensitivity. Among these, genes associated with mitochondria showed the most enrichment. We also found that LiPF6 is more toxic to yeast than lithium chloride (LiCl) or sodium hexafluorophosphate (NaPF6). Physiological analysis showed that a high concentration of LiPF6 caused mitochondrial damage, reactive oxygen species (ROS) accumulation, and ATP content changes. Compared with the results of previous genome-wide screening for LiCl-sensitive mutants, we found that oxidative phosphorylation-related mutants were specifically hypersensitive to LiPF6. In these deletion mutants, LiPF6 treatment resulted in higher ROS production and reduced ATP levels, suggesting that oxidative phosphorylation-related genes were important for counteracting LiPF6-induced toxicity. Taken together, our results identified genes specifically involved in LiPF6-modulated toxicity, and demonstrated that oxidative stress and ATP imbalance maybe the driving factors in governing LiPF6-induced toxicity.

scholarly journals Functional screening for resistance genes against trichothecenes in the library of Saccharomyces cerevisiae deletion mutants

2013 ◽  
Vol 63 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Naoko TAKAHASHI-ANDO ◽  
Akira TANAKA ◽  
Yohsuke SEKIMOTO ◽  
Kohta YAMAUCHI ◽  
Akinobu ECHIGO ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Resistance Genes ◽  
Deletion Mutants ◽  
Functional Screening ◽  
Screening For Resistance

Pleiotropic drug-resistance attenuated genomic library improves elucidation of drug mechanisms

2015 ◽  
Vol 11 (11) ◽  
pp. 3129-3136 ◽  
Author(s):  
Namal V. C. Coorey ◽  
James H. Matthews ◽  
David S. Bellows ◽  
Paul H. Atkinson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Mechanism Of Action ◽  
Gene Deletion ◽  
Genomic Library ◽  
Deletion Mutants ◽  
Pleiotropic Drug Resistance ◽  
Genome Wide

Identifying Saccharomyces cerevisiae genome-wide gene deletion mutants that confer hypersensitivity to a xenobiotic aids the elucidation of its mechanism of action (MoA).

scholarly journals Regulation of Copper Metabolism by Nitrogen Utilization in Saccharomyces cerevisiae

2021 ◽  
Vol 7 (9) ◽  
pp. 756
Author(s):  
Suzie Kang ◽  
Hyewon Seo ◽  
Min-Gyu Lee ◽  
Cheol-Won Yun
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Iron Uptake ◽  
Nitrogen Starvation ◽  
Copper Metabolism ◽  
Nitrogen Utilization ◽  
Deletion Mutants ◽  
Uptake System ◽  
Wild Type ◽  
High Affinity ◽  
Uptake Activity

To understand the relationship between carbon or nitrogen utilization and iron homeostasis, we performed an iron uptake assay with several deletion mutants with partial defects in carbon or nitrogen metabolism. Among them, some deletion mutants defective in carbon metabolism partially and the MEP2 deletion mutant showed lower iron uptake activity than the wild type. Mep2 is known as a high-affinity ammonia transporter in Saccharomyces cerevisiae. Interestingly, we found that nitrogen starvation resulted in lower iron uptake activity than that of wild-type cells without downregulation of the genes involved in the high-affinity iron uptake system FET3/FTR1. However, the gene expression of FRE1 and CTR1 was downregulated by nitrogen starvation. The protein level of Ctr1 was also decreased by nitrogen starvation, and addition of copper to the nitrogen starvation medium partially restored iron uptake activity. However, the expression of MAC1, which is a copper-responsive transcriptional activator, was not downregulated by nitrogen starvation at the transcriptional level but was highly downregulated at the translational level. Mac1 was downregulated dramatically under nitrogen starvation, and treatment with MG132, which is an inhibitor of proteasome-dependent protein degradation, partially attenuated the downregulation of Mac1. Taken together, these results suggest that nitrogen starvation downregulates the high-affinity iron uptake system by degrading Mac1 in a proteasome-dependent manner and eventually downregulates copper metabolism.

scholarly journals Isolation and characterization of the RAD3 gene of Saccharomyces cerevisiae and inviability of rad3 deletion mutants

1983 ◽  
Vol 80 (18) ◽  
pp. 5680-5684 ◽  
Author(s):  
D. R. Higgins ◽  
S. Prakash ◽  
P. Reynolds ◽  
R. Polakowska ◽  
S. Weber ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Deletion Mutants ◽  
Isolation And Characterization

Revelation of molecular basis for chromium toxicity by phenotypes of Saccharomyces cerevisiae gene deletion mutants

Metallomics ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 542-550 ◽  
Author(s):  
Adam J. Johnson ◽  
Filip Veljanoski ◽  
Patrick. J. O'Doherty ◽  
Mohammad S. Zaman ◽  
Gayani Petersingham ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Basis ◽  
Gene Deletion ◽  
Deletion Mutants ◽  
Chromium Toxicity

Improved activity of the Cel5A endoglucanase in Saccharomyces cerevisiae deletion mutants defective in oxidative stress defense mechanisms

2015 ◽  
Vol 37 (5) ◽  
pp. 1081-1089
Author(s):  
Guochao Wu ◽  
Jintang Sun ◽  
Shanshan Yu ◽  
Quanchao Dong ◽  
Guoqiang Zhuang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Defense Mechanisms ◽  
Deletion Mutants ◽  
Oxidative Stress Defense ◽  
Stress Defense
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