Time-dependent effects of parathyroid hormone and prostaglandin E2 on DNA synthesis by periosteal cells from embryonic chick calvaria

1994 ◽  
Vol 55 (3) ◽  
pp. 208-215 ◽  
Author(s):  
A. Scutt ◽  
C. Duvos ◽  
J. Lauber ◽  
H. Mayer
Keyword(s):  
Parathyroid Hormone ◽  
Dna Synthesis ◽  
Prostaglandin E2 ◽  
Time Dependent ◽  
Embryonic Chick ◽  
Periosteal Cells

Development of the cyclic AMP response to parathyroid hormone and prostaglandin E2 in the embryonic chick limb

1981 ◽  
Vol 33 (1) ◽  
pp. 641-648 ◽  
Author(s):  
Curtis L. Parker ◽  
David M. Biddulph ◽  
Timothy A. Ballard
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Prostaglandin E2 ◽  
Embryonic Chick

DNA synthesis in cultured embryonic chick cerebellar cells

1980 ◽  
Vol 187 (2) ◽  
pp. 473-475 ◽  
Author(s):  
Karen L. Freeman ◽  
David I. Gottlieb
Keyword(s):  
Dna Synthesis ◽  
Embryonic Chick ◽  
Cerebellar Cells

Synthetic Human Parathyroid Hormone Fragment Stimulates Prostaglandin E2 Synthesis by Chick Calvariae*

Endocrinology ◽  
1987 ◽  
Vol 120 (1) ◽  
pp. 63-70 ◽  
Author(s):  
C. Y. YANG ◽  
W. A. GONNERMAN ◽  
L. TAYLOR ◽  
R. B. NIMBERG ◽  
P. R. POLGAR
Keyword(s):  
Parathyroid Hormone ◽  
Prostaglandin E2 ◽  
Human Parathyroid Hormone

Parathyroid hormone co-stimulation of hepatocyte proliferation is sensitive to protein kinase A and calcium channel inhibitors

1996 ◽  
Vol 16 (4) ◽  
pp. 343-350 ◽  
Author(s):  
George G. Skouteris
Keyword(s):  
Parathyroid Hormone ◽  
Dna Synthesis ◽  
Specific Inhibitor ◽  
Hepatocyte Proliferation ◽  
Antisense Oligodeoxynucleotide ◽  
Inositol 1,4,5 Trisphosphate ◽  
Calcium Channel Inhibitors ◽  
Fos Expression ◽  
Kinase A ◽  
Stimulation Of

Parathyroid hormone (PTH) mobilises calcium in the hepatocyte, an effect which is abolished by verapamil and staurosporine. In our study parathyroid hormone was shown to act additively to dHGF in inducing hepatocyte DNA synthesis. It is also shown that PTH induced the production of inositol 1,4,5 trisphosphate (IP3) and c-fos expression at early times in culture. Co-incubation of PTH and dHGF with a c-fos antisense oligodeoxynucleotide inhibited hepatocyte DNA synthesis, indicating that the additive effect of PTH is correlated with the induction of c-fos. H-89, a PKA specific inhibitor, inhibited the PTH effect on IP3 production as well as the PTH effect on hepatocyte DNA synthesis. Verapamil and staurosporine also inhibited the PTH effect in dHGF-induced DNA synthesis. Therefore it is suggested that PKA mediated at a great extent the co-stimulatory effects of PTH on hepatocyte proliferation via IP3 production.

scholarly journals THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS

1966 ◽  
Vol 28 (3) ◽  
pp. 473-487 ◽  
Author(s):  
Joan Abbott ◽  
Howard Holtzer
Keyword(s):  
Dna Synthesis ◽  
Chondroitin Sulfate ◽  
Collagen Synthesis ◽  
Dna Interaction ◽  
Cell Multiplication ◽  
Phenotypic Traits ◽  
Embryonic Chick ◽  
Differentiated Cells ◽  
Genetically Determined

Observations were made on the behavior of chondrocytes grown under various conditions in vitro. The chondrocytes in 10-day embryonic chick vertebrae were grown as cultures of intact vertebrae, as pellets of chondrocytes liberated from their matrix, and as monodispersed cells plated out on plasma clots. Cartilage matrix was stained metachromatically with toluidine blue. Radioautographs were made of incorporated H3-thymidine, H3-proline, and S35-sulfate to determine the extent of DNA synthesis, collagen synthesis, and chondroitin sulfate synthesis, respectively. Chondrocytes in intact vertebrae or in pellets are rounded and actively synthesizing chondroitin sulfate and collagen. There is little DNA synthesis by cells in either vertebrae or pellets. Chondrocytes grown as monodisperse cells rapidly cease synthesizing cytologically detectable chondroitin sulfate and are induced to synthesize DNA and divide. There is a change in the shape of these chondrocytes from a rounded to a more stellate condition which accompanies the shift in metabolic activity. Conversely, when the cells attain a certain cell density, they reacquire a rounded shape, cease dividing, and again synthesize chondroitin sulfate. Clusters of chondrocytes synthesize more chondroitin sulfate than isolated chondrocytes. It is concluded that most chondrocytes synthesizing chondroitin sulfate do not concurrently synthesize DNA. Interaction between associated chondrocytes is important in inducing and maintaining chondroitin sulfate synthesis in genetically determined chondrocytes. Failure of interaction between chondrocytes leads to DNA synthesis and cell multiplication.

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