State of the rigid-layer in cell walls of some gram-negative bacteria

1972 ◽  
Vol 83 (4) ◽  
pp. 332-346 ◽  
Author(s):  
H. H. Martin ◽  
H. D. Heilmann ◽  
H. J. Preusser
Keyword(s):  
Cell Walls ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Rigid Layer

LYSOZYME SENSITIVITY OF THE CELL WALL OF PSEUDO-MONAS AERUGINOSA: FURTHER EVIDENCE FOR THE ROLE OF THE NON-PEPTIDOGLYCAN COMPONENTS IN CELL WALL RIGIDITY

1966 ◽  
Vol 12 (1) ◽  
pp. 105-108 ◽  
Author(s):  
K. Jane Carson ◽  
R. G. Eagon
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Wall ◽  
Electron Micrographs ◽  
Cell Walls ◽  
Gram Negative Bacteria ◽  
Normal Cells ◽  
Thin Sections ◽  
Gram Negative ◽  
Cell Wall Rigidity

Electron micrographs of thin sections of normal cells of Pseudomonas aeruginosa showed the cell walls to be convoluted and to be composed of two distinct layers. Electron micrographs of thin sections of lysozyme-treated cells of P. aeruginosa showed (a) that the cell walls lost much of their convoluted nature; (b) that the layers of the cell walls became diffuse and less distinct; and (c) that the cell walls became separated from the protoplasts over extensive cellular areas. These results suggest that the peptidoglycan component of the unaltered cell walls of P. aeruginosa is sensitive to lysozyme. Furthermore, it appears that the peptidoglycan component is not solely responsible for the rigidity of the cell walls of Gram-negative bacteria.

The effects of phospholipid depletion on the cleavage pattern of the cell walls of frozen Gram-negative bacteria

1973 ◽  
Vol 19 (8) ◽  
pp. 1056-1057 ◽  
Author(s):  
A. Forge ◽  
J. W. Costerton
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Cytoplasmic Membrane ◽  
Gram Negative Bacteria ◽  
Cleavage Pattern ◽  
Gram Negative ◽  
Whole Cells ◽  
The Cross ◽  
Double Track ◽  
Chloroform Methanol

Extraction of whole cells of the marine pseudomonad (B-16) with chloroform–methanol causes the disappearance of the cleavage planes, and the cross-sectioned profile of both the cytoplasmic membrane and the double-track layer of the cell wall.

A General Structure for Cell Walls of Gram-Negative Bacteria

Nature ◽  
1962 ◽  
Vol 195 (4840) ◽  
pp. 516-517 ◽  
Author(s):  
PATRICIA H. CLARKE ◽  
M. D. LILLY
Keyword(s):  
Cell Walls ◽  
General Structure ◽  
Gram Negative Bacteria ◽  
Gram Negative

Gram staining v1

2021 ◽  
Author(s):  
lydiariver not provided
Keyword(s):  
Cell Walls ◽  
Group B Streptococcus ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Staining ◽  
Acetone Treatment ◽  
Gram Negative Organisms ◽  
Group B ◽  
Gram Positive Cocci

Gram staining is one of the first techniques used for the identification of group B Streptococcus agalactiae and one would expect to see gram-positive cocci under the microscope. The technique consists of applying a series of colorants and bleaches (acetone), which interact with the lipids of the membranes of gram-positive and gram-negative bacteria. The cell walls of gram-positive organisms retain the dye after acetone treatment and appear purple in color, whereas gram-negative organisms become discolored after acetone treatment and appear pink.

scholarly journals Amount of peptidoglycan in cell walls of gram-negative bacteria.

1991 ◽  
Vol 173 (23) ◽  
pp. 7684-7691 ◽  
Author(s):  
F B Wientjes ◽  
C L Woldringh ◽  
N Nanninga
Keyword(s):  
Cell Walls ◽  
Gram Negative Bacteria ◽  
Gram Negative

LYSOZYME IN THE BACTERIOLYSIS OF GRAM-NEGATIVE BACTERIA: I. MORPHOLOGICAL CHANGES DURING USE OF NAKAMURA'S TECHNIQUE

1957 ◽  
Vol 3 (1) ◽  
pp. 13-21 ◽  
Author(s):  
E. A. Grula ◽  
S. E. Hartsell
Keyword(s):  
Electron Microscope ◽  
Cell Walls ◽  
Light Transmission ◽  
Morphological Changes ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Gram Negative ◽  
Whole Cells ◽  
Sequence Of Events ◽  
Reversible Hydration

Whole cells or the cell walls of 22 species representing 11 genera of Gram-negative bacteria were exposed to lysozyme using a modified Nakamura technique. The cell walls of all organisms contain the lysozyme substrate in differing amounts (two Brucella spp., Proteus vulgaris X-19, and Vibrio cholerae Chicago are possible exceptions) when evaluated spectrophotometrically and with the electron microscope. Using the latter technique, the sequence of events during bacteriolysis with lysozyme was observed. After exposure to lysozyme in saline, with the modified Nakamura technique and the phase microscope, cells were observed to either swell or shrink depending on the pH of the menstruum. This phenomenon apparently involves reversible hydration of cell proteins with concomitant changes in light transmission.

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