In vivo complementation analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardtii

1986 ◽  
Vol 10 (5) ◽  
pp. 397-403 ◽  
Author(s):  
Emilio Fern�ndez ◽  
Ren� F. Matagne
Keyword(s):  
Nitrate Reductase ◽  
Chlamydomonas Reinhardtii ◽  
Complementation Analysis

scholarly journals To Divide or Not to Divide? How Deuterium Affects Growth and Division of Chlamydomonas reinhardtii

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 861
Author(s):  
Veronika Kselíková ◽  
Vilém Zachleder ◽  
Kateřina Bišová
Keyword(s):  
Cell Cycle ◽  
Chlamydomonas Reinhardtii ◽  
Cell Cycle Progression ◽  
Model Organism ◽  
Cycle Progression ◽  
Synchronous Cultures ◽  
Multiple Fission ◽  
First Choice ◽  
High Doses

Extensive in vivo replacement of hydrogen by deuterium, a stable isotope of hydrogen, induces a distinct stress response, reduces cell growth and impairs cell division in various organisms. Microalgae, including Chlamydomonas reinhardtii, a well-established model organism in cell cycle studies, are no exception. Chlamydomonas reinhardtii, a green unicellular alga of the Chlorophyceae class, divides by multiple fission, grows autotrophically and can be synchronized by alternating light/dark regimes; this makes it a model of first choice to discriminate the effect of deuterium on growth and/or division. Here, we investigate the effects of high doses of deuterium on cell cycle progression in C. reinhardtii. Synchronous cultures of C. reinhardtii were cultivated in growth medium containing 70 or 90% D2O. We characterize specific deuterium-induced shifts in attainment of commitment points during growth and/or division of C. reinhardtii, contradicting the role of the “sizer” in regulating the cell cycle. Consequently, impaired cell cycle progression in deuterated cultures causes (over)accumulation of starch and lipids, suggesting a promising potential for microalgae to produce deuterated organic compounds.

scholarly journals Creation of a Chloroplast Microsatellite Reporter for Detection of Replication Slippage in Chlamydomonas reinhardtii

2008 ◽  
Vol 7 (4) ◽  
pp. 639-646 ◽  
Author(s):  
Monica GuhaMajumdar ◽  
Ethan Dawson-Baglien ◽  
Barbara B. Sears
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Forensic Analysis ◽  
Direct Repeats ◽  
Reporter Construct ◽  
Chloroplast Microsatellite ◽  
Replication Slippage ◽  
Reading Frame ◽  
Viable Cells ◽  
Short Tandem

ABSTRACT Microsatellites are composed of short tandem direct repeats; deletions or duplications of those repeats through the process of replication slippage result in microsatellite instability relative to other genomic loci. Variation in repeat number occurs so frequently that microsatellites can be used for genotyping and forensic analysis. However, an accurate assessment of the rates of change can be difficult because the presence of many repeats makes it difficult to determine whether changes have occurred through single or multiple events. The current study was undertaken to experimentally assess the rates of replication slippage that occur in vivo in the chloroplast DNA of Chlamydomonas reinhardtii. A reporter construct was created in which a stretch of AAAG repeats was inserted into a functional gene to allow changes to be observed when they occurred at the synthetic microsatellite. Restoration of the reading frame occurred through replication slippage in 15 of every million viable cells. Since only one-third of the potential insertion/deletion events would restore the reading frame, the frequency of change could be deduced to be 4.5 × 10−5. Analysis of the slippage events showed that template slippage was the primary event, resulting in deletions rather than duplications. These findings contrasted with events observed in Escherichia coli during maintenance of the plasmid, where duplications were the rule.

scholarly journals In Vivo Interactions between Photosynthesis, Mitorespiration, and Chlororespiration in Chlamydomonas reinhardtii

2002 ◽  
Vol 129 (4) ◽  
pp. 1921-1928 ◽  
Author(s):  
Laurent Cournac ◽  
Gwendal Latouche ◽  
Zoran Cerovic ◽  
Kevin Redding ◽  
Jacques Ravenel ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii

In vivo assay of nitrate reductase in tea (Camellia sinensis L.) leaf and root

1987 ◽  
Vol 41 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Tarun Ch. Sarmah ◽  
Mahinath Dev Choudhury ◽  
Monoranjan Goswami
Keyword(s):  
Nitrate Reductase ◽  
Camellia Sinensis ◽  
In Vivo Assay

SEASONAL PATTERNS OF DENITRIFICATION AND LEAF NITRATE REDUCTASE ACTIVITY IN A CORN FIELD

1978 ◽  
Vol 58 (2) ◽  
pp. 283-285 ◽  
Author(s):  
D. G. PATRIQUIN ◽  
J. C. MacKINNON ◽  
K. I. WILKIE
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
General Trend ◽  
Reductase Activity ◽  
Seasonal Patterns ◽  
Assay Procedure ◽  
Acetylene Blockage Technique ◽  
Acetylene Blockage ◽  
Leaf Nitrate

Denitrification in soil around the bases of corn stalks, determined by the "acetylene blockage technique," exhibited a general trend of decline from June to September. Leaf nitrate reductase activity, determined by an in vivo assay procedure, was low in June and July, and then exhibited a pronounced maximum at the time of tasselling.

scholarly journals Analysis of Flagellar Phosphoproteins from Chlamydomonas reinhardtii

2009 ◽  
Vol 8 (7) ◽  
pp. 922-932 ◽  
Author(s):  
Jens Boesger ◽  
Volker Wagner ◽  
Wolfram Weisheit ◽  
Maria Mittag
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Protein Phosphorylation ◽  
Proteome Analysis ◽  
Physiological Status ◽  
Phosphorylation Sites ◽  
Cell Organelles ◽  
Flagellar Proteins ◽  
Reversible Protein Phosphorylation ◽  
Cilia And Flagella

ABSTRACT Cilia and flagella are cell organelles that are highly conserved throughout evolution. For many years, the green biflagellate alga Chlamydomonas reinhardtii has served as a model for examination of the structure and function of its flagella, which are similar to certain mammalian cilia. Proteome analysis revealed the presence of several kinases and protein phosphatases in these organelles. Reversible protein phosphorylation can control ciliary beating, motility, signaling, length, and assembly. Despite the importance of this posttranslational modification, the identities of many ciliary phosphoproteins and knowledge about their in vivo phosphorylation sites are still missing. Here we used immobilized metal affinity chromatography to enrich phosphopeptides from purified flagella and analyzed them by mass spectrometry. One hundred forty-one phosphorylated peptides were identified, belonging to 32 flagellar proteins. Thereby, 126 in vivo phosphorylation sites were determined. The flagellar phosphoproteome includes different structural and motor proteins, kinases, proteins with protein interaction domains, and many proteins whose functions are still unknown. In several cases, a dynamic phosphorylation pattern and clustering of phosphorylation sites were found, indicating a complex physiological status and specific control by reversible protein phosphorylation in the flagellum.

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