scholarly journals Methanol metabolism in a peroxisome-deficient mutant of Hansenula polymorpha: a physiological study

1991 ◽  
Vol 156 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Ida J. van der Klei ◽  
Wim Harder ◽  
Marten Veenhuis
Keyword(s):  
Hansenula Polymorpha ◽  
Physiological Study ◽  
Deficient Mutant ◽  
Methanol Metabolism

Regulation of methanol metabolism in the yeast Hansenula polymorpha

1987 ◽  
Vol 147 (4) ◽  
pp. 375-382 ◽  
Author(s):  
W. de Koning ◽  
M. A. G. Gleeson ◽  
W. Harder ◽  
L. Dijkhuizen
Keyword(s):  
Hansenula Polymorpha ◽  
Methanol Metabolism

scholarly journals Peroxisome reintroduction in Hansenula polymorpha requires Pex25 and Rho1

2011 ◽  
Vol 193 (5) ◽  
pp. 885-900 ◽  
Author(s):  
Ruchi Saraya ◽  
Arjen M. Krikken ◽  
Marten Veenhuis ◽  
Ida J. van der Klei
Keyword(s):  
Mutant Strain ◽  
De Novo ◽  
Hansenula Polymorpha ◽  
Protein Family ◽  
Yeast Cells ◽  
Deficient Mutant ◽  
Wild Type ◽  
Double Deletion ◽  
Insight Into

We identified two proteins, Pex25 and Rho1, which are involved in reintroduction of peroxisomes in peroxisome-deficient yeast cells. These are, together with Pex3, the first proteins identified as essential for this process. Of the three members of the Hansenula polymorpha Pex11 protein family—Pex11, Pex25, and Pex11C—only Pex25 was required for reintroduction of peroxisomes into a peroxisome-deficient mutant strain. In peroxisome-deficient pex3 cells, Pex25 localized to structures adjacent to the ER, whereas in wild-type cells it localized to peroxisomes. Pex25 cells were not themselves peroxisome deficient but instead contained a slightly increased number of peroxisomes. Interestingly, pex11 pex25 double deletion cells, in which both peroxisome fission (due to the deletion of PEX11) and reintroduction (due to deletion of PEX25) was blocked, did display a peroxisome-deficient phenotype. Peroxisomes reappeared in pex11 pex25 cells upon synthesis of Pex25, but not of Pex11. Reintroduction in the presence of Pex25 required the function of the GTPase Rho1. These data therefore provide new and detailed insight into factors important for de novo peroxisome formation in yeast.

Clinical Physiological Study of the Action of BC 105 (Sandoz, Basle) in the Interval Therapy of Migraine

1974 ◽  
Vol 11 (6) ◽  
pp. 353-362 ◽  
Author(s):  
O. Starý ◽  
M. Janský ◽  
Š. Figar ◽  
J. Stein
Keyword(s):  
Physiological Study ◽  
Interval Therapy
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