Hair cell regeneration in the chick inner ear following acoustic trauma: ultrastructural and immunohistochemical studies

1995 ◽  
Vol 281 (3) ◽  
pp. 435-443 ◽  
Author(s):  
Masanori Umemoto ◽  
Masafumi Sakagami ◽  
Keijiro Fukazawa ◽  
Kentaro Ashida ◽  
Takeshi Kubo ◽  
...  
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Acoustic Trauma ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Immunohistochemical Studies

Hair cell regeneration in the chick inner ear following acoustic trauma: ultrastructural and immunohistochemical studies

1995 ◽  
Vol 281 (3) ◽  
pp. 435-443
Author(s):  
Masanori Umemoto ◽  
Masafumi Sakagami ◽  
Keijiro Fukazawa ◽  
Kentaro Ashida ◽  
Takeshi Kubo ◽  
...  
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Acoustic Trauma ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Immunohistochemical Studies

Hair cell regeneration in the inner ear

1994 ◽  
Vol 111 (3) ◽  
pp. 281-301 ◽  
Author(s):  
T TSUE ◽  
E OESTERLE ◽  
E RUBEL
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

Delta1 expression during avian hair cell regeneration

Development ◽  
1999 ◽  
Vol 126 (5) ◽  
pp. 961-973 ◽  
Author(s):  
J.S. Stone ◽  
E.W. Rubel
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Hair Cells ◽  
Cell Injury ◽  
Basilar Papilla ◽  
Cell Regeneration ◽  
Mrna Levels ◽  
Hair Cell Regeneration ◽  
Drug Induced ◽  
In Cells

Postembryonic production of hair cells, the highly specialized receptors for hearing, balance and motion detection, occurs in a precisely controlled manner in select species, including avians. Notch1, Delta1 and Serrate1 mediate cell specification in several tissues and species. We examined expression of the chicken homologs of these genes in the normal and drug-damaged chick inner ear to determine if signaling through this pathway changes during hair cell regeneration. In untreated post-hatch chicks, Delta1 mRNA is abundant in a subpopulation of cells in the utricle, which undergoes continual postembryonic hair cell production, but it is absent from all cells in the basilar papilla, which is mitotically quiescent. By 3 days after drug-induced hair cell injury, Delta1 expression is highly upregulated in areas of cell proliferation in both the utricle and basilar papilla. Delta1 mRNA levels are elevated in progenitor cells during DNA synthesis and/or gap 2 phases of the cell cycle and expression is maintained in both daughter cells immediately after mitosis. Delta1 expression remains upregulated in cells that differentiate into hair cells and is downregulated in cells that do not acquire the hair cell fate. Delta1 mRNA levels return to normal by 10 days after hair cell injury. Serrate1 is expressed in both hair cells and support cells in the utricle and basilar papilla, and its expression does not change during the course of drug-induced hair cell regeneration. In contrast, Notch1 expression, which is limited to support cells in the quiescent epithelium, is increased in post-M-phase cell pairs during hair cell regeneration. This study provides initial evidence that Delta-Notch signaling may be involved in maintaining the correct cell types and patterns during postembryonic replacement of sensory epithelial cells in the chick inner ear.

scholarly journals Notch Inhibition Induces Cochlear Hair Cell Regeneration and Recovery of Hearing after Acoustic Trauma

Neuron ◽  
2013 ◽  
Vol 78 (2) ◽  
pp. 403 ◽  
Author(s):  
Kunio Mizutari ◽  
Masato Fujioka ◽  
Makoto Hosoya ◽  
Naomi Bramhall ◽  
Hirotaka James Okano ◽  
...  
Keyword(s):  
Hair Cell ◽  
Acoustic Trauma ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Cochlear Hair Cell ◽  
Notch Inhibition

scholarly journals Applying genomics to the avian inner ear: Development of subtractive cDNA resources for exploring sensory function and hair cell regeneration

Genomics ◽  
2006 ◽  
Vol 87 (6) ◽  
pp. 801-808 ◽  
Author(s):  
R. David Hawkins ◽  
Cynthia A. Helms ◽  
Julia B. Winston ◽  
Mark E. Warchol ◽  
Michael Lovett
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Sensory Function ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Ear Development ◽  
Inner Ear Development ◽  
Avian Inner Ear

Factors controlling hair-cell regeneration/repair in the inner ear

1998 ◽  
Vol 8 (4) ◽  
pp. 480-487 ◽  
Author(s):  
Hinrich Staecker ◽  
Thomas R Van De Water
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Cell Regeneration ◽  
Hair Cell Regeneration

scholarly journals Macrophages Respond Rapidly to Ototoxic Injury of Lateral Line Hair Cells but Are Not Required for Hair Cell Regeneration

2021 ◽  
Vol 14 ◽  
Author(s):  
Mark E. Warchol ◽  
Angela Schrader ◽  
Lavinia Sheets
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Lateral Line ◽  
Hair Cells ◽  
Control Fish ◽  
Cell Regeneration ◽  
Cellular Interactions ◽  
Hair Cell Regeneration ◽  
Genetic Ablation ◽  
Larval Zebrafish

The sensory organs of the inner ear contain resident populations of macrophages, which are recruited to sites of cellular injury. Such macrophages are known to phagocytose the debris of dying cells but the full role of macrophages in otic pathology is not understood. Lateral line neuromasts of zebrafish contain hair cells that are nearly identical to those in the inner ear, and the optical clarity of larval zebrafish permits direct imaging of cellular interactions. In this study, we used larval zebrafish to characterize the response of macrophages to ototoxic injury of lateral line hair cells. Macrophages migrated into neuromasts within 20 min of exposure to the ototoxic antibiotic neomycin. The number of macrophages in the near vicinity of injured neuromasts was similar to that observed near uninjured neuromasts, suggesting that this early inflammatory response was mediated by “local” macrophages. Upon entering injured neuromasts, macrophages actively phagocytosed hair cell debris. The injury-evoked migration of macrophages was significantly reduced by inhibition of Src-family kinases. Using chemical-genetic ablation of macrophages before the ototoxic injury, we also examined whether macrophages were essential for the initiation of hair cell regeneration. Results revealed only minor differences in hair cell recovery in macrophage-depleted vs. control fish, suggesting that macrophages are not essential for the regeneration of lateral line hair cells.

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