Cloning of a DNA fragment from Cephalosporium acremonium which functions as an autonomous replication sequence in yeast

1984 ◽  
Vol 8 (3) ◽  
pp. 155-163 ◽  
Author(s):  
Paul L. Skatrud ◽  
Stephen W. Queener
Keyword(s):  
Cephalosporium Acremonium ◽  
Autonomous Replication ◽  
Dna Fragment ◽  
Autonomous Replication Sequence

Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae

1981 ◽  
Vol 1 (6) ◽  
pp. 535-543
Author(s):  
G B Kiss ◽  
A A Amin ◽  
R E Pearlman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Frequency ◽  
Deoxyribonucleic Acid ◽  
Tetrahymena Thermophila ◽  
The Other ◽  
Yeast Cells ◽  
Origin Of Replication ◽  
Autonomous Replication ◽  
Coding Regions ◽  
Dna Fragment

Plasmids containing the nontranscribed central and terminal, but not the coding, regions of the extrachromosomal ribosomal deoxyribonucleic acid (rDNA) of Tetrahymena thermophila are capable of autonomous replication in Saccharomyces cerevisiae. These plasmids transform S. cerevisiae at high frequency; transformants are unstable in the absence of selection, and plasmids identical to those used for transformation were isolated from the transformed yeast cells. One plasmid contains a 1.85-kilobase Tetrahymena DNA fragment which includes the origin of bidirectional replication of the extrachromosomal rDNA. The other region of Tetrahymena rDNA allowing autonomous replication of plasmids in S. cerevisiae is a 650-base pair, adenine plus thymine-rich segment from the rDNA terminus. Neither of these Tetrahymena fragments shares obvious sequence homology with the origin of replication of the S. cerevisiae 2-microns circle plasmid or with ars1, an S. cerevisiae chromosomal replicator.

Cloning an Autonomous Replication Sequence fromAspergillus oryzae

1993 ◽  
Vol 45 (6) ◽  
pp. 209-211 ◽  
Author(s):  
K. Kudo ◽  
A. Okoshi ◽  
R. Usami ◽  
K. Horikoshi
Keyword(s):  
Autonomous Replication ◽  
Autonomous Replication Sequence

scholarly journals Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

2020 ◽  
Author(s):  
William T. Molin ◽  
Allison Yaguchi ◽  
Mark Blenner ◽  
Christopher Saski
Keyword(s):  
Sequence Analysis ◽  
Dna Unwinding ◽  
Amaranthus Palmeri ◽  
Yeast Plasmid ◽  
Autonomous Replication ◽  
Close Proximity ◽  
Self Replication ◽  
Autonomous Replication Sequence

Abstract Objective The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.

scholarly journals Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

2020 ◽  
Author(s):  
William T. Molin ◽  
Allison Yaguchi ◽  
Mark Blenner ◽  
Christopher Saski
Keyword(s):  
Sequence Analysis ◽  
Dna Unwinding ◽  
Amaranthus Palmeri ◽  
Yeast Plasmid ◽  
Autonomous Replication ◽  
Close Proximity ◽  
Self Replication ◽  
Autonomous Replication Sequence

Abstract Objective: The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results: Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A+T/G+C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.

scholarly journals Cloning and characterization of an autonomous replication sequence from Coxiella burnetii.

1994 ◽  
Vol 176 (17) ◽  
pp. 5233-5243 ◽  
Author(s):  
M Suhan ◽  
S Y Chen ◽  
H A Thompson ◽  
T A Hoover ◽  
A Hill ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Autonomous Replication ◽  
Autonomous Replication Sequence

scholarly journals Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.

1981 ◽  
Vol 1 (6) ◽  
pp. 535-543 ◽  
Author(s):  
G B Kiss ◽  
A A Amin ◽  
R E Pearlman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Frequency ◽  
Deoxyribonucleic Acid ◽  
Tetrahymena Thermophila ◽  
The Other ◽  
Yeast Cells ◽  
Origin Of Replication ◽  
Autonomous Replication ◽  
Coding Regions ◽  
Dna Fragment

Plasmids containing the nontranscribed central and terminal, but not the coding, regions of the extrachromosomal ribosomal deoxyribonucleic acid (rDNA) of Tetrahymena thermophila are capable of autonomous replication in Saccharomyces cerevisiae. These plasmids transform S. cerevisiae at high frequency; transformants are unstable in the absence of selection, and plasmids identical to those used for transformation were isolated from the transformed yeast cells. One plasmid contains a 1.85-kilobase Tetrahymena DNA fragment which includes the origin of bidirectional replication of the extrachromosomal rDNA. The other region of Tetrahymena rDNA allowing autonomous replication of plasmids in S. cerevisiae is a 650-base pair, adenine plus thymine-rich segment from the rDNA terminus. Neither of these Tetrahymena fragments shares obvious sequence homology with the origin of replication of the S. cerevisiae 2-microns circle plasmid or with ars1, an S. cerevisiae chromosomal replicator.

A Study of DNA Fragment Assembly Algorithms

10.26524/jap2 ◽  
2016 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
Satyanarayana Reddy Beeram ◽  
Edara Srinivasa Reddy
Keyword(s):  
Fragment Assembly ◽  
Dna Fragment ◽  
Assembly Algorithms ◽  
Dna Fragment Assembly
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