Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37° C and 27° C

1981 ◽  
Vol 129 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Reeta Taneja ◽  
G. K. Khuller
1969 ◽  
Vol 15 (9) ◽  
pp. 1112-1114 ◽  
Author(s):  
Wilbur D. Jones Jr. ◽  
R. Edward Beam

Using the agglutination test and the indirect fluorescent antibody test it was demonstrated that lysogenization of the rough variant of Mycobacterium smegmatis ATCC 607 by mycobacteriophages D29 and B4 was accompanied by the appearance of new bacterial antigens. Each phage mediated a different bacterial antigen. That the new bacterial antigens were phage mediated was demonstrated by the loss of the new antigens when the bacterial culture was "cured" of the prophage.


1985 ◽  
Vol 63 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Sandeep Mahajan ◽  
G. K. Khuller

Cerulenin inhibited the lipid synthesis of Mycobacterium smegmatis ATCC 607 over the range of 0.5–1.8 μg/mL with complete inhibition at 1.8 μg/mL, as monitored by [14C]glycerol incorporation into lipids. Exogenous fatty acids failed to restore the lipid synthesis at 1.8 μg/mL; however, the addition of palmitic acid to the growth medium partially restored the lipid synthesis when cerulenin concentration was decreased to 1.6 μg/mL. Fatty acid analysis of cerulenin plus palmitic acid supplemented cultures revealed that exogenously supplied fatty acid was incorporated into cellular phospholipids. Further investigations with 1.6 μg/mL of cerulenin and [14C]acetate and [32P]orthophosphoric acid showed that cerulenin inhibited the synthesis of saturated plus unsaturated fatty acids and phospholipids. Pulse–chase studies with [14C]acetate revealed decreased synthesis and degradation of each of the phospholipid components.


1988 ◽  
Vol 41 (5) ◽  
pp. 675-683 ◽  
Author(s):  
KAYOKO SUZUKAKE-TSUCHIYA ◽  
MAKOTO HORI ◽  
NOBUYOSHI SHIMADA ◽  
MASA HAMADA

Microbiology ◽  
1981 ◽  
Vol 124 (1) ◽  
pp. 203-206
Author(s):  
A. SUBRAMONIAM ◽  
D. SUBRAHMANYAM

1981 ◽  
Vol 19 (2) ◽  
pp. 346-348 ◽  
Author(s):  
J. O. Kilburn ◽  
K. Takayama ◽  
E. L. Armstrong ◽  
J. Greenberg

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