Partial purification and properties of extracellular chitinase produced by Acremonium obclavatum, an antagonist to the groundnut rust, Puccinia arachidis

1994 ◽  
Vol 10 (3) ◽  
pp. 342-345 ◽  
Author(s):  
K. R. Gunaratna ◽  
R. Balasubramanian
Keyword(s):  
Partial Purification ◽  
Puccinia Arachidis ◽  
Purification And Properties ◽  
Groundnut Rust

Partial purification and properties of apoplastic β-1,3 glucanases of groundnut leaves treated with glucan isolated from a biocontrol agent, Acremonium obclavatum

2000 ◽  
Vol 78 (2) ◽  
pp. 168-174 ◽  
Author(s):  
M Sathiyabama ◽  
R Balasubramanian
Keyword(s):  
Arachis Hypogaea ◽  
Partial Purification ◽  
Ph Optimum ◽  
Native Page ◽  
Puccinia Arachidis ◽  
Ammonium Sulphate Precipitation ◽  
Arachis Hypogaea L ◽  
Purification And Properties ◽  
Molecular Masses ◽  
Electrophoretic Techniques

Apoplastic β-1,3 glucanases (G1, G2) of Arachis hypogaea L. (peanut) leaves treated with glucan have been partially purified by ammonium sulphate precipitation, sephadex G-100, CM-Sephadex, DEAE-Sephadex chromatography, and preparative native PAGE electrophoretic techniques. The pI values of purified enzymes G1 and G2 were near 8 and 4, respectively. The apparent molecular masses of purified glucanases G1 and G2 from glucan-treated peanut leaves were 36 and 34 kDa, respectively. Both isoforms (G1 and G2) showed their pH optimum of 5.0 and temperature optimum of 40°C. The partially purified enzymes hydrolysed laminarin better than other substrates and inhibited uredospore germination of Puccinia arachidis. Both isoforms (G1 and G2) inhibited spore germination of some biocontrol agents such as Acremonium obclavatum W. Gams, Myrothecium verrucaria (Alb. Schw.) Ditmer, Fusarium solani (Mart.) Sacc., and Trichoderma harzianum Rifai.Key words: Acremonium obclavatum, Arachis hypogaea, β-1,3 glucanase, glucan, inhibition, Puccinia arachidis.

Partial purification and properties of an endo-xylanase from cucumber seeds

1991 ◽  
Vol 81 (3) ◽  
pp. 327-334 ◽  
Author(s):  
Cesar V. Mujer ◽  
Dale W. Kretchman ◽  
A. Raymond Miller
Keyword(s):  
Partial Purification ◽  
Purification And Properties

Partial purification and properties of a 36-kDa 1-aminocyclopropane-l-carboxylate N-malonyltransferase from mung bean

1995 ◽  
Vol 94 (4) ◽  
pp. 629-634 ◽  
Author(s):  
Mohamed Benichou ◽  
Gracia Martinez-Reina ◽  
Felix Romojaro ◽  
Jean-Claude Pech ◽  
Alain Latche
Keyword(s):  
Mung Bean ◽  
Partial Purification ◽  
Purification And Properties

scholarly journals Partial purification and properties of a pre-mRNA splicing activity.

1985 ◽  
Vol 260 (2) ◽  
pp. 1096-1102
Author(s):  
P R DiMaria ◽  
G Kaltwasser ◽  
C J Goldenberg
Keyword(s):  
Mrna Splicing ◽  
Partial Purification ◽  
Purification And Properties

Partial Purification and Properties of Human Brain Aldehyde Dehydrogenases

1985 ◽  
Vol 45 (6) ◽  
pp. 1903-1910 ◽  
Author(s):  
Jacques-Andre Maring ◽  
Richard A. Deitrich ◽  
Roger Little
Keyword(s):  
Human Brain ◽  
Partial Purification ◽  
Aldehyde Dehydrogenases ◽  
Purification And Properties
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