Acetate oxidation to CO2 via a citric acid cycle involving an ATP-citrate lyase: a mechanism for the synthesis of ATP via substrate level phosphorylation in Desulfobacter postgatei growing on acetate and sulfate

1987 ◽  
Vol 148 (3) ◽  
pp. 202-207 ◽  
Author(s):  
D. M�ller ◽  
R. Schauder ◽  
G. Fuchs ◽  
R. K. Thauer
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Acetate Oxidation ◽  
Atp Citrate Lyase ◽  
Acid Cycle ◽  
Substrate Level Phosphorylation ◽  
Citrate Lyase ◽  
Substrate Level ◽  
Desulfobacter Postgatei

scholarly journals Mitochondrial diaphorases as NAD + donors to segments of the citric acid cycle that support substrate‐level phosphorylation yielding ATP during respiratory inhibition

2014 ◽  
Vol 28 (4) ◽  
pp. 1682-1697 ◽  
Author(s):  
Gergely Kiss ◽  
Csaba Konrad ◽  
Issa Pour‐Ghaz ◽  
Josef J. Mansour ◽  
Béata Németh ◽  
...  
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Acid Cycle ◽  
Substrate Level Phosphorylation ◽  
Respiratory Inhibition ◽  
Substrate Level

scholarly journals Citric Acid Cycle in the Hyperthermophilic Archaeon Pyrobaculum islandicum Grown Autotrophically, Heterotrophically, and Mixotrophically with Acetate

2006 ◽  
Vol 188 (12) ◽  
pp. 4350-4355 ◽  
Author(s):  
Yajing Hu ◽  
James F. Holden
Keyword(s):  
Citric Acid ◽  
Yeast Extract ◽  
Growth Rates ◽  
Citric Acid Cycle ◽  
Citrate Synthase ◽  
Autotrophic Growth ◽  
Acid Cycle ◽  
Hyperthermophilic Archaeon ◽  
Citrate Lyase ◽  
Pyrobaculum Islandicum

ABSTRACT The hyperthermophilic archaeon Pyrobaculum islandicum uses the citric acid cycle in the oxidative and reductive directions for heterotrophic and autotrophic growth, respectively, but the control of carbon flow is poorly understood. P. islandicum was grown at 95°C autotrophically, heterotrophically, and mixotrophically with acetate, H2, and small amounts of yeast extract and with thiosulfate as the terminal electron acceptor. The autotrophic growth rates and maximum concentrations of cells were significantly lower than those in other media. The growth rates on H2 and 0.001% yeast extract with and without 0.05% acetate were the same, but the maximum concentration of cells was fourfold higher with acetate. There was no growth with acetate if 0.001% yeast extract was not present, and addition of H2 to acetate-containing medium greatly increased the growth rates and maximum concentrations of cells. P. islandicum cultures assimilated 14C-labeled acetate in the presence of H2 and yeast extract with an efficiency of 55%. The activities of 11 of 19 enzymes involved in the central metabolism of P. islandicum were regulated under the three different growth conditions. Pyruvate synthase and acetate:coenzyme A (CoA) ligase (ADP-forming) activities were detected only in heterotrophically grown cultures. Citrate synthase activity decreased in autotrophic and acetate-containing cultures compared to the activity in heterotrophic cultures. Acetylated citrate lyase, acetate:CoA ligase (AMP forming), and phosphoenolpyruvate carboxylase activities increased in autotrophic and acetate-containing cultures. Citrate lyase activity was higher than ATP citrate synthase activity in autotrophic cultures. These data suggest that citrate lyase and AMP-forming acetate:CoA ligase, but not ATP citrate synthase, work opposite citrate synthase to control the direction of carbon flow in the citric acid cycle.

scholarly journals STUDIES ON THE MECHANISM OF ACETATE OXIDATION BY BACTERIA

1951 ◽  
Vol 35 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Samuel J. Ajl
Keyword(s):  
Citric Acid ◽  
Carbon Source ◽  
Dicarboxylic Acid ◽  
Citric Acid Cycle ◽  
Acetate Oxidation ◽  
Acid Cycle

The data presented in this paper indicate operation of different mechanisms for acetate oxidation by A. aerogenes, depending on the carbon source used for growth. The mechanism for citrate-grown cells appears to involve a conventional citric acid cycle, whereas acetate-grown cells appear to incorporate acetate carbon more readily via a dicarboxylic acid cycle.

Acetate oxidation to CO2 in anaerobic bacteria via a novel pathway not involving reactions of the citric acid cycle

1986 ◽  
Vol 145 (2) ◽  
pp. 162-172 ◽  
Author(s):  
Rolf Schauder ◽  
Bernhard Eikmanns ◽  
Rudolf K. Thauer ◽  
Fritz Widdel ◽  
Georg Fuchs
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Anaerobic Bacteria ◽  
Acetate Oxidation ◽  
Acid Cycle

EFFECT OF SERUM GONADOTROPHIN ON DEHYDROGENASES OF THE CITRIC ACID CYCLE IN THE OVARY OF THE RAT

1963 ◽  
Vol 42 (4) ◽  
pp. 480-484 ◽  
Author(s):  
B. Eckstein ◽  
R. Landsberg
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Age Groups ◽  
Succinic Dehydrogenase ◽  
Immature Rat ◽  
Acid Cycle ◽  
Immature Rats ◽  
Rat Ovary

ABSTRACT The succinic, malic and isocitric dehydrogenases in the ovary of immature and mature, normal and serum gonadotrophin injected rats were examined. The Qo2 of these enzymes were markedly enhanced in the gonadotrophin injected rats of both age groups, except in the case of succinic dehydrogenase in the ovary of the immature rats, where a slight non-significant decrease was noted. It is concluded that in the mature rat ovary, gonadotrophin administration stimulates the activity of all the examined dehydrogenases of the citric acid cycle, whereas in the immature rat ovary, at least the isocitric- and malic dehydrogenases are thus stimulated.

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