Autoradiographic study of glycoprotein synthesis during embryonic epidermogenesis of the mouse in vitro

1983 ◽  
Vol 275 (2) ◽  
pp. 109-113 ◽  
Author(s):  
Rainer Herken ◽  
Ulrich Schultz-Ehrenburg
Keyword(s):  
Autoradiographic Study ◽  
Glycoprotein Synthesis

Autoradiographic study of macromolecular synthesis in the fusion epithelium of the developing rat primary palate in vitro

Development ◽  
1979 ◽  
Vol 50 (1) ◽  
pp. 145-154
Author(s):  
Alvaro A. Figueroa ◽  
Robert M. Pratt
Keyword(s):  
Autoradiographic Study ◽  
Whole Embryo Culture ◽  
Glycoprotein Synthesis ◽  
Palate Development ◽  
Molecular Events ◽  
Secondary Palate ◽  
Primary Palate ◽  
Epithelial Fusion ◽  
Developing Rat

The facial processes involved in primary palate formation undergo epithelial fusion in a manner morphologically analogous to that observed during secondary palate formation. We have used whole embryo culture to analyze the synthesis of macromolecules (DNA, protein, glycoprotein) in the primary palate, based on the incorporation of various labeled precursors. The results of this study demonstrate that changes in the synthesis of macromolecules occur during the fusion of the facial processes, which resemble those previously reported to occur during secondary palate development. These changes include cessation of DNA synthesis in cells in a restricted zone of the epithelium, concomitant with maintenance of glycoprotein synthesis. These findings indicate that the molecular events underlying the development of the primary and secondary palate may be similar.

scholarly journals Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

1978 ◽  
Vol 76 (2) ◽  
pp. 418-429 ◽  
Author(s):  
D M Nelson ◽  
A C Enders ◽  
B F King
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Rough Endoplasmic Reticulum ◽  
Autoradiographic Study ◽  
Leucine Incorporation ◽  
Electron Microscope Autoradiography ◽  
Glycoprotein Synthesis ◽  
Placental Villi ◽  
Syncytial Trophoblast

Electron microscope autoradiography was used to study glycoprotein synthesis in cellular trophoblast (cytotrophoblast) and syncytial trophoblast of term human placental villi incubated in vitro with D-[1-3H]galactose ([3H]gal). Autoradiographs were analyzed using the hypothetical grain analysis of Blackett and Parry (1973. J. Cell Biol. 57:9-15). The results of this study indicated that [3H]gal incorporation into term placental villi was predominantly localized to cytotrophoblast. Utilization of [3H]gal by term syncytial trophoblast was extremely low and yielded too few grains for a quantitative grain analysis. This result is in striking contrast to that found in the preceding study of [3H]leucine incorporation (Nelson, D. M., A. C. Enders, and B. F. King. 1978). Within cytotrophoblast, the rough endoplasmic reticulum incorporated the most [3H]gal into glycoprotein. The Golgi apparatus was another site of [3H]gal incorporation. The vast majority of the [3H]gal incorporated into cytotrophoblast during the pulse incubation remained intracellular through the duration of the experiment. There was little autoradiographic evidence for secretion of tritiated macromolecules. Cytotrophoblast incubated for the longest time period studied (4 h+) showed a substantial concentration of tritiated macromolecules in the Golgi complex and in the ground plasm but not in the rough endoplasmic reticulum.

scholarly journals The in vitro localization of H3 estradiol in human prostatic carcinoma.An electron microscopic autoradiographic study

Cancer ◽  
1973 ◽  
Vol 31 (3) ◽  
pp. 682-688 ◽  
Author(s):  
Akhouri A. Sinha ◽  
Clyde E. Blackard ◽  
Richard P. Doe ◽  
Ulysses S. Seal
Keyword(s):  
Autoradiographic Study ◽  
Electron Microscopic

Autoradiographic study of chick limb cartilage in vivo and in vitro and its response to mitomycin C (MMC)

1975 ◽  
Vol 93 (3) ◽  
pp. 440-446 ◽  
Author(s):  
Shamer Singh ◽  
D.N. Sinha ◽  
G.C. Prasad
Keyword(s):  
Mitomycin C ◽  
Autoradiographic Study
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