?-mating-type-specific suppression and a-mating-type-specific enhancement by tunicamycin of sexual agglutinability in the yeast Saccharomyces cervisiae

1984 ◽  
Vol 138 (4) ◽  
pp. 310-314 ◽  
Author(s):  
Satoshi Hasegawa ◽  
Naohiko Yanagishima
Keyword(s):  
Mating Type ◽  
Specific Suppression ◽  
Sexual Agglutinability ◽  
Specific Enhancement ◽  
Saccharomyces Cervisiae

scholarly journals Cyclic AMP enhances the sexual agglutinability of Chlamydomonas flagella.

1989 ◽  
Vol 109 (1) ◽  
pp. 247-252 ◽  
Author(s):  
U W Goodenough
Keyword(s):  
Cell Wall ◽  
Cyclic Amp ◽  
Mating Type ◽  
Feedback System ◽  
Intracellular Camp ◽  
Camp Generation ◽  
Sexual Agglutinability ◽  
Single Mating ◽  
Wall Loss ◽  
Camp Levels

Sexual adhesion between Chlamydomonas reinhardtii gametes elicits a rise in intracellular cAMP levels, and exogenous elevation of intracellular cAMP levels in gametes of a single mating type induces such mating responses as cell wall loss, flagellar tip activation, and mating structure activation (Pasquale, S. M., and U. W. Goodenough. 1987. J. Cell Biol. 105:2279-2292). Here evidence is presented that sexual adhesion mobilizes agglutinin to the flagellar surface, and that this mobilization can be induced by exogenous presentation of cAMP to gametes of a single mating type. It is proposed that Chlamydomonas adhesion entails a positive feedback system--initial contacts stimulate the presentation of additional agglutinin--and that this feedback is mediated by adhesion-induced cAMP generation.

scholarly journals Tipping and mating-structure activation induced in Chlamydomonas gametes by flagellar membrane antisera.

1978 ◽  
Vol 79 (3) ◽  
pp. 680-693 ◽  
Author(s):  
U W Goodenough ◽  
D Jurivich
Keyword(s):  
Mating Type ◽  
Dynamic Properties ◽  
Cross Linking ◽  
Mating Types ◽  
Vegetative Cells ◽  
Sexual Agglutination ◽  
Sexual Agglutinability ◽  
Flagellar Membrane ◽  
Mutant Strains ◽  
High Concentrations

Antisera raised against vegetative and gametic flagella of Chlamydomonas reinhardi have been used to probe dynamic properties of the flagellar membranes. The antisera, which agglutinate cells via their flagella, associate with antigens that are present on both vegetative and gametic membranes and on membranes of both mating types (mt+ and mt-). Gametic cells respond to antibody presentation very differently from vegetative cells, mobilizing even high concentrations of antibody towards the flagellar tips; the possibility is discussed that such "tipping" ability reflects a differentiated gametic property relevant to sexual agglutinability. Gametic cells also respond to antibody agglutination by activating their mating structures, the mt+ reaction involving a rapid polymerization of microfilaments. Several impotent mt+ mutant strains that fail to agglutinate sexually are also activated by the antisera and procede to form zygotes with normal mt- gametes. Fusion does not occur between activated cells of like mating type. Monovalent (Fab) preparations of the antibody fail to activate mt+ gametes, suggesting that the cross-linking properties of the antisera are essential for their ability to mimic, or bypass, sexual agglutination.

Development of a PCR assay for amplification of mating-type loci of Alternaria spp. and related fungi.

2013 ◽  
Vol 65 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Philipp B. Gannibal ◽  
Igor A. Kazartsev
Keyword(s):  
Mating Type ◽  
Pcr Assay
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