Comparative studies of pollen and fluorescent dye transport by bumble bees visiting Erythronium grandiflorum

Oecologia ◽  
1986 ◽  
Vol 69 (4) ◽  
pp. 561-566 ◽  
Author(s):  
James D. Thomson ◽  
Mary V. Price ◽  
Nickolas M. Waser ◽  
Donald A. Stratton
Keyword(s):  
Comparative Studies ◽  
Fluorescent Dye ◽  
Bumble Bees ◽  
Dye Transport ◽  
Erythronium Grandiflorum

BIOCHEMICAL SYSTEMATICS AND THE HIGHER CATEGORIES

1973 ◽  
Vol 105 (9) ◽  
pp. 1223-1233 ◽  
Author(s):  
W. P. Stephen
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Comparative Studies ◽  
Amino Acid Sequences ◽  
Bumble Bees ◽  
Objective Data ◽  
Amino Acid Sequencing ◽  
Taxonomic Problem ◽  
Higher Categories ◽  
The Stability

AbstractThe premises for biochemical systematics are outlined, including the demonstration of colinearity between polypeptides and the nucleotide sequence of the gene, and subsequent comparative studies based on amino acid sequencing of proteins. Distinct differences in the stability in the amino acid sequences among various proteins demands that the systematist select those most appropriate to the taxonomic problem to be resolved. As sequencing is a difficult and time consuming task, an alternative analytical means of obtaining objective data by which taxa may be phenetically compared is proposed. Electrophoretic studies on the enzyme GDH from cockroaches and bumble bees are outlined and the use of the data in superspecific classifications is documented.

3-Dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 164-165
Author(s):  
Thomas J. Deerinck ◽  
Maryann E. Martone ◽  
Varda Lev-Ram ◽  
David P. L. Green ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Reactive Oxygen ◽  
Confocal Laser Scanning Microscope ◽  
Fluorescent Dye ◽  
Confocal Laser ◽  
3 Dimensional ◽  
Voltage Electron ◽  
Dimensional Distribution ◽  
Electron Microscopes ◽  
New Generation

The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.

Further Comparative Studies on Ascites in Liver and Heart Disease

1950 ◽  
Vol 16 (1) ◽  
pp. 91-103 ◽  
Author(s):  
John A. Layne ◽  
F.R. Schemm ◽  
W.W. Hurst
Keyword(s):  
Heart Disease ◽  
Comparative Studies

A Reply to Kuder's Criticism of SVIB-KOIS Comparative Studies

1972 ◽  
Vol 5 (1) ◽  
pp. 306-309
Author(s):  
Arthur J. O'Shea ◽  
Mervin D. Lynch ◽  
Thomas F. Harrington
Keyword(s):  
Comparative Studies

The Use of Radioiodinated Congo Red in the Study of Amyloidosis

1963 ◽  
Vol 03 (01) ◽  
pp. 25-38
Author(s):  
Manuel Tubis ◽  
William Blahd ◽  
John Endow
Keyword(s):  
Rheumatoid Arthritis ◽  
Multiple Myeloma ◽  
Comparative Studies ◽  
Congo Red ◽  
Half Time ◽  
Positive Correlation ◽  
Autopsy Findings ◽  
Percentage Removal

SummaryA study of the removal of I131-labeled Congo red from the blood of amyloid, non-amyloid, multiple myeloma, rheumatoid arthritis and other patients is presented. The percentage removal of the labeled dye shows the same variation reported by many other workers using Bennhold’s test and its modifications.However, there seems to be a positive correlation between the percentage removal of the labeled dye and the presence of amyloid as revealed by biopsy and autopsy. The half-time of disappearance is also correlated with the amyloidosis.The availability of the I131-labeled dye permits the use of very small weights of the dye thereby drastically reducing the possibility of toxic and sometimes fatal reactions encountered with the unlabeled dye. The I131 present permits easy quantitation of the dye in the blood without separation of plasma and obviates the need of fasting. It also permits external counting and scanning of deposits in the organs containing the dye.The availability and use of the labeled dye may stimulate more comparative studies of the removal of the dye from the blood correlated with biopsy and autopsy findings.

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