Separation and characterization of two chorismate-mutase isoenzymes from Nicotiana silvestris

Planta ◽  
1984 ◽  
Vol 162 (2) ◽  
pp. 109-116 ◽  
Author(s):  
S. K. Goers ◽  
R. A. Jensen
Keyword(s):  
Chorismate Mutase

scholarly journals Feedback Inhibition of Chorismate Mutase/Prephenate Dehydrogenase (TyrA) of Escherichia coli: Generation and Characterization of Tyrosine-Insensitive Mutants

2005 ◽  
Vol 71 (11) ◽  
pp. 7224-7228 ◽  
Author(s):  
Tina Lütke-Eversloh ◽  
Gregory Stephanopoulos
Keyword(s):  
Escherichia Coli ◽  
Dna Sequences ◽  
Molecular Level ◽  
Feedback Inhibition ◽  
Feedback Regulation ◽  
The Other ◽  
Chorismate Mutase ◽  
Amino Acid Substitutions ◽  
Prephenate Dehydrogenase

ABSTRACT In order to get insights into the feedback regulation by tyrosine of the Escherichia coli chorismate mutase/prephenate dehydrogenase (CM/PDH), which is encoded by the tyrA gene, feedback-inhibition-resistant (fbr) mutants were generated by error-prone PCR. The tyrA fbr mutants were selected by virtue of their resistance toward m-fluoro-d,l-tyrosine, and seven representatives were characterized on the biochemical as well as on the molecular level. The PDH activities of the purified His6-tagged TyrA proteins exhibited up to 35% of the enzyme activity of TyrAWT, but tyrosine did not inhibit the mutant PDH activities. On the other hand, CM activities of the TyrAfbr mutants were similar to those of the TyrAWT protein. Analyses of the DNA sequences of the tyrA genes revealed that tyrA fbr contained amino acid substitutions either at Tyr263 or at residues 354 to 357, indicating that these two sites are involved in the feedback inhibition by tyrosine.

Characterization of the secreted chorismate mutase from the pathogen Mycobacterium tuberculosis

FEBS Journal ◽  
2004 ◽  
Vol 272 (2) ◽  
pp. 375-389 ◽  
Author(s):  
Severin Sasso ◽  
Chandra Ramakrishnan ◽  
Marianne Gamper ◽  
Donald Hilvert ◽  
Peter Kast
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Chorismate Mutase

scholarly journals Design, selection, and characterization of a split chorismate mutase

2010 ◽  
Vol 19 (5) ◽  
pp. 1000-1010 ◽  
Author(s):  
Manuel M. Müller ◽  
Hajo Kries ◽  
Eva Csuhai ◽  
Peter Kast ◽  
Donald Hilvert
Keyword(s):  
Chorismate Mutase ◽  
Design Selection

Characterization of a chorismate mutase from the potato cyst nematode Globodera pallida

2002 ◽  
Vol 4 (1) ◽  
pp. 43-50 ◽  
Author(s):  
John T. Jones ◽  
Cleber Furlanetto ◽  
Erin Bakker ◽  
Bryony Banks ◽  
Vivian Blok ◽  
...  
Keyword(s):  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Globodera Pallida ◽  
Chorismate Mutase
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