A method for the hyaluronic acid synthetase assay in human skin

1982 ◽  
Vol 273-273 (3-4) ◽  
pp. 199-204 ◽  
Author(s):  
Hannu Larjava ◽  
Jari Vaarala ◽  
Heikki Saarni ◽  
Risto Penttinen ◽  
V�in� K. Hopsu-Havu
Keyword(s):  
1949 ◽  
Vol 27e (1) ◽  
pp. 43-57 ◽  
Author(s):  
R. H. Pearce ◽  
E. M. Watson

The mean values and standard deviations for the concentrations of the hyaluronic acid and chondroitin sulphuric acid fractions isolated from 11 specimens of human skin were 24.5 ± 5.7 and 26.2 ± 4.7 mgm. per 100 gm. of fresh tissue, respectively.The individual differences in the concentrations of these substances were masked by the technical errors. The small variations in moisture and ash content of the skin did not contribute appreciably to the uncertainty of the concentration values. The purity of the products, determined by their amino sugar content, approximated 60%, which value compared favorably with those obtained by other workers.The content of hexosamine and uronic acid identified both fractions as acid mucopolysaccharides. The release of reducing substances, including N-acetyl hexosamine, from the hyaluronic acid fraction by hyaluronidase paralleled the action of the enzyme upon hyaluronate from umbilical cord. The chondroitin sulphuric acid fraction, which was relatively resistant to the action of hyaluronidase, was assumed to be identical with the chondroitin sulphatelike substance that Meyer and Chaffee isolated from pig skin.


Dermatology ◽  
2013 ◽  
Vol 228 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Christian Tran ◽  
Pierre Carraux ◽  
Patrick Micheels ◽  
Gürkan Kaya ◽  
Denis Salomon

2009 ◽  
Vol 38 (3) ◽  
pp. 1098-1110 ◽  
Author(s):  
Antonio Peramo ◽  
Cynthia L. Marcelo ◽  
Steven A. Goldstein ◽  
David C. Martin

1990 ◽  
Vol 172 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Toshiya Nakamura ◽  
Keiichi Takagaki ◽  
Kohmei Kubo ◽  
Akiko Morikawa ◽  
Shinri Tamura ◽  
...  

Author(s):  
Antonio Scarano ◽  
Andrea Sbarbati ◽  
Roberto Amore ◽  
Eugenio Luigi Iorio ◽  
Giueseppe Ferraro ◽  
...  

2017 ◽  
Vol Volume11 ◽  
pp. 669-676 ◽  
Author(s):  
Magdalena Donejko ◽  
Edyta Rysiak ◽  
Elżbieta Galicka ◽  
Robert Terlikowski ◽  
Edyta Głażewska ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document