Induction of stress proteins by sodium chloride treatment in Bacillus subtilis

1988 ◽  
Vol 150 (6) ◽  
pp. 564-566 ◽  
Author(s):  
Michael Hecker ◽  
Christine Heim ◽  
Uwe V�lker ◽  
Lothar W�lfel
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Chloride ◽  
Stress Proteins ◽  
Chloride Treatment

scholarly journals Mechanisms of Adaptation to Nitrosative Stress in Bacillus subtilis

2007 ◽  
Vol 189 (8) ◽  
pp. 3063-3071 ◽  
Author(s):  
Annika Rogstam ◽  
Jonas T. Larsson ◽  
Peter Kjelgaard ◽  
Claes von Wachenfeldt
Keyword(s):  
Bacillus Subtilis ◽  
Stress Proteins ◽  
Nitrosative Stress ◽  
Vital Role ◽  
Null Mutant ◽  
Truncated Hemoglobin ◽  
Sigma B ◽  
Nitrosonium Cation ◽  
Mechanisms Of Adaptation ◽  
Increased Sensitivity

ABSTRACT Bacteria use a number of mechanisms for coping with the toxic effects exerted by nitric oxide (NO) and its derivatives. Here we show that the flavohemoglobin encoded by the hmp gene has a vital role in an adaptive response to protect the soil bacterium Bacillus subtilis from nitrosative stress. We further show that nitrosative stress induced by the nitrosonium cation donor sodium nitroprusside (SNP) leads to deactivation of the transcriptional repressor NsrR, resulting in derepression of hmp. Nitrosative stress induces the sigma B-controlled general stress regulon. However, a sigB null mutant did not show increased sensitivity to SNP, suggesting that the sigma B-dependent stress proteins are involved in a nonspecific protection against stress whereas the Hmp flavohemoglobin plays a central role in detoxification. Mutations in the yjbIH operon, which encodes a truncated hemoglobin (YjbI) and a predicted 34-kDa cytosolic protein of unknown function (YjbH), rendered B. subtilis hypersensitive to SNP, suggesting roles in nitrosative stress management.

scholarly journals Specific and general stress proteins in Bacillus subtilis - a two-dimensional protein electrophoresis study

Microbiology ◽  
1997 ◽  
Vol 143 (3) ◽  
pp. 999-1017 ◽  
Author(s):  
J. Bernhardt ◽  
U. Volker ◽  
A. Volker ◽  
H. Antelmann ◽  
R. Schmid ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stress Proteins ◽  
Protein Electrophoresis ◽  
Two Dimensional ◽  
General Stress

scholarly journals Plant Response to Saline Substrates VII. Growth and Ion Uptake Throughout Plant Development in Two Varieties of Hordeum Vulgare

1965 ◽  
Vol 18 (4) ◽  
pp. 763 ◽  
Author(s):  
H Greenway
Keyword(s):  
Sodium Chloride ◽  
Hordeum Vulgare ◽  
Plant Development ◽  
Plant Response ◽  
Ion Uptake ◽  
Main Experiment ◽  
Grain Formation ◽  
Chloride Treatment

In the main experiment, sodium chloride treatment was imposed on two varieties of H. vulgare during early tillering and continued until grain formation.

scholarly journals Plant Response to Saline Substrates II. Chloride, Sodium, and Potassium Uptake and Translocation in Young Plants of Hordeum Vulgare During and After a Short Sodium Chloride Treatment

1962 ◽  
Vol 15 (1) ◽  
pp. 39 ◽  
Author(s):  
H Greenway
Keyword(s):  
Sodium Chloride ◽  
Hordeum Vulgare ◽  
Salinity Stress ◽  
Chloride Concentration ◽  
Plant Response ◽  
Potassium Uptake ◽  
Sodium Chloride Concentration ◽  
Subsequent Response ◽  
Chloride Treatment ◽  
Sodium And Potassium

Young barley plants, Hordeum vulgare cv. Chevron, were subjected to a sodium chloride concentration of 100 m-equiv/l. In a "continued" treatment, the salinity stress was maintained for 15 days. In a "removed" treatment, sodium chloride was removed from the substrate after 5 days, and the subsequent response was studied over a period of 10 days.

scholarly journals Bacillus subtilis Tolerance of Moderate Concentrations of Rifampin Involves the σB-Dependent General and Multiple Stress Response

2002 ◽  
Vol 184 (2) ◽  
pp. 459-467 ◽  
Author(s):  
Julia Elisabeth Bandow ◽  
Heike Brötz ◽  
Michael Hecker
Keyword(s):  
Bacillus Subtilis ◽  
Stress Response ◽  
Rna Polymerase ◽  
Stress Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Growth Arrest ◽  
Wild Type ◽  
General Stress ◽  
Inhibition Of Growth ◽  
Increased Sensitivity

ABSTRACT Low concentrations of the RNA polymerase inhibitor rifampin added to an exponentially growing culture of Bacillus subtilis led to an instant inhibition of growth. Survival experiments revealed that during the growth arrest the cells became tolerant to the antibiotic and the culture was able to resume growth some time after rifampin treatment. l-[35S]methionine pulse-labeled protein extracts were separated by two-dimensional polyacrylamide gel electrophoresis to investigate the change in the protein synthesis pattern in response to rifampin. The σB-dependent general stress proteins were found to be induced after treatment with the antibiotic. Part of the oxidative stress signature was induced as indicated by the catalase KatA and MrgA. The target protein of rifampin, the β subunit (RpoB) of the DNA-dependent RNA polymerase, and the flagellin protein Hag belonging to the σD regulon were also induced. The rifampin-triggered growth arrest was extended in a sigB mutant in comparison to the wild-type strain, and the higher the concentration, the more pronounced this effect was. Activity of the RsbP energy-signaling phosphatase in the σB signal transduction network was also important for this protection against rifampin, but the RsbU environmental signaling phosphatase was not required. The sigB mutant strain was less capable of growing on rifampin-containing agar plates. When plated from a culture that had already reached stationary phase without previous exposure to the antibiotic during growth, the survival rate of the wild type exceeded that of the sigB mutant by a factor of 100. We conclude that the general stress response of B. subtilis is induced by rifampin depending on RsbP activity and that loss of SigB function causes increased sensitivity to the antibiotic.

Bromism: The Sodium Chloride Treatment

BMJ ◽  
1926 ◽  
Vol 1 (3405) ◽  
pp. 636-636
Author(s):  
R. W. Nichol
Keyword(s):  
Sodium Chloride ◽  
Chloride Treatment

Effect of 0.25 N sodium chloride treatment on DNA denaturation in situ in thymus lymphocytes

1976 ◽  
Vol 100 (2) ◽  
pp. 393-396 ◽  
Author(s):  
Z. Darżynkiewicz ◽  
F. Traganos ◽  
T. Sharpless ◽  
M.R. Melamed
Keyword(s):  
Sodium Chloride ◽  
Dna Denaturation ◽  
Chloride Treatment

scholarly journals Bacillus subtilis improves maize tolerance to salinity

2018 ◽  
Vol 48 (8) ◽  
Author(s):  
Nathalia Calhabeu Ferreira ◽  
Rita de Cassia Lima Mazzuchelli ◽  
Ana Claudia Pacheco ◽  
Fabio Fernando de Araujo ◽  
Jadson Emanuel Lopes Antunes ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Chloride ◽  
Plant Growth ◽  
Water Content ◽  
Relative Water Content ◽  
Saline Stress ◽  
Biochemical Responses ◽  
Relative Water ◽  
Four Levels ◽  
Tolerance To Salinity

ABSTRACT: The aim of this study was to evaluate the biochemical responses of maize, under saline stress, inoculated with Bacillus subtilis. Four levels of salinity were assessed: 0mM, 50mM, 100mM, and 200mM of sodium chloride (NaCl). Saline conditions influenced negatively maize growth. However, the inoculation of B. subtilis improved the plant growth at highest level of NaCl. Chlorophyll content decreased while proline increased in inoculated plants submitted to highest salt levels. Also, B. subtilis increased the relative water content in leaves. B. subtilis improves the plant growth under salinity and ameliorates the biochemical damages in maize.

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