In vivo 31P NMR studies on the role of the vacuole in phosphate metabolism in yeasts

1983 ◽  
Vol 134 (4) ◽  
pp. 270-275 ◽  
Author(s):  
K. Nicolay ◽  
W. A. Scheffers ◽  
P. M. Bruinenberg ◽  
R. Kaptein
1983 ◽  
Vol 33 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Shoji NARUSE ◽  
Soshun TAKADA ◽  
Izumi KOIZUKA ◽  
Hiroshi WATARI

1991 ◽  
Vol 201 (1) ◽  
pp. 283-287 ◽  
Author(s):  
Helena SANTOS ◽  
Paula FARELEIRA ◽  
Carmen PEDREGAL ◽  
Jean LeGALL ◽  
Antonio V. XAVIER

1989 ◽  
Vol 482 (1) ◽  
pp. 1-11 ◽  
Author(s):  
J.A. Stolk ◽  
J.I. Olsen ◽  
P.M. Reeves ◽  
M. Chen ◽  
C. Perry ◽  
...  
Keyword(s):  
31P Nmr ◽  

1987 ◽  
Vol 62 (4) ◽  
pp. 1569-1574 ◽  
Author(s):  
D. P. Younkin ◽  
L. C. Wagerle ◽  
B. Chance ◽  
J. Maria ◽  
M. Delivoria-Papadopoulos

We measured cerebral phosphocreatine (PCr), inorganic phosphate (Pi), ATP, and intracellular pH (pHi) with in vivo phosphorus nuclear magnetic resonance (NMR) during 10- to 15-min periods of reversible hypoxic hypoxia in 20 newborn lambs (1–11 days). There was a significant correlation between arterial O2 partial pressure (PaO2) and the PCr/Pi ratio or pHi; however, between PaO2 130–33 mmHg, metabolite changes were not significant. PCr/Pi and pHi decreased significantly when PaO2 was lowered below 33 and 28 mmHg, respectively. After recovery, metabolite ratios and pHi returned to base-line values within 5 min. During the early phases of hypoxia and recovery, there were large fluctuations in metabolites and pHi, indicating that mitochondrial reactions were not in a steady state. After several minutes of hypoxia or recovery, PCr/Pi and pHi stabilized, suggesting steady state kinetics for mitochondrial respiration. NMR is extremely sensitive to changes in mitochondrial oxygenation, and stable PCr/Pi and pHi indicate that O2 tension in cerebral mitochondria of the newborn lamb is constant between PaO2 of 30 and 140 mmHg.


FEBS Letters ◽  
1983 ◽  
Vol 159 (1-2) ◽  
pp. 207-210 ◽  
Author(s):  
Klaus J. Neurohr ◽  
Gerald Gollin ◽  
Eugene J. Barrett ◽  
Robert G. Shulman

1986 ◽  
Vol 21 (9) ◽  
pp. S28
Author(s):  
P Mills ◽  
C Buonomo ◽  
J H Anderson ◽  
R Pillai ◽  
S Rajan ◽  
...  
Keyword(s):  
31P Nmr ◽  

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