Relationship between substrate concentration, growth rate, and respiration rate of Escherichia coli in continuous culture

1964 ◽  
Vol 48 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Karl L. Schulze ◽  
Robert S. Lipe
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Continuous Culture ◽  
Respiration Rate ◽  
Substrate Concentration

Evolution of Escherichia coli During Growth in a Constant Environment

Genetics ◽  
1987 ◽  
Vol 116 (3) ◽  
pp. 349-358
Author(s):  
Robert B Helling ◽  
Christopher N Vargas ◽  
Julian Adams
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Continuous Culture ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Constant Environment ◽  
Single Clone ◽  
Mixed Populations

ABSTRACT Populations of Escherichia coli, initiated with a single clone and maintained for long periods in glucose-limited continuous culture, developed extensive polymorphisms. In one population, examined after 765 generations, two majority and two minority types were identified. Stable mixed populations were reestablished from the isolated strains. Factors involved in the development of this polymorphism included differences in the maximum specific growth rate and in the transport of glucose, and excretion of metabolites by some clones which were utilized by minority clones.

scholarly journals Respiration Rate, Growth Rate and the Accumulation of Streptomycin in Escherichia coli

Microbiology ◽  
1985 ◽  
Vol 131 (10) ◽  
pp. 2573-2579
Author(s):  
M. E. MUIR ◽  
M. BALLESTEROS ◽  
B. J. WALLACE
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Respiration Rate

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

Effect of growth rate on the physiological rates of a chemostat-grown rotifer Encentrum linnhei

1988 ◽  
Vol 68 (1) ◽  
pp. 165-177 ◽  
Author(s):  
J. M. Scott
Keyword(s):  
Growth Rate ◽  
Oxygen Consumption ◽  
Steady State ◽  
Respiration Rate ◽  
Clearance Rate ◽  
Ingestion Rate ◽  
Physiological State ◽  
Growth Efficiency ◽  
Theoretical Figure ◽  
Marine Rotifer

The physiological rates of a normally omnivorous marine rotifer, Encentrum linnhei, were measured under the steady-state chemostat conditions in which the physiological state of the food-algae was kept constant whilst the rotifer growth rate was changed to preset levels. The specific clearance rate ranged between 50 and 100 μl/μg rotifer C/day (1.5–3.0 μ/rot/day) and varied hyperbolically with growth rate, a similar curve was obtained with the specific ingestion rate which varied between 1–2 μg C/μg rot C/day. A mean respiration rate of 0.45 μg C/μg rot C/day was obtained from oxygen consumption measurements. About 60‰ of ingested energy was found to be egested as paniculate matter and 9–4 °0 dissipated as heat, the latter comparing with a theoretical figure of 4–5‰.From rates, transfer efficiencies were obtained giving a mean net growth efficiency (K2) of 38‰ and a mean overall growth efficiency (K1 of 15‰. A curvilinear increase of Kl with growth rate contrasts with linear and hyperbolic responses found with brachionid rotifers.

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