Inhibition of nitrogenase activity by metronidazole in Rhodopseudomonas capsulata

1981 ◽  
Vol 129 (5) ◽  
pp. 344-348 ◽  
Author(s):  
Bruce C. Kelley ◽  
D. J. D. Nicholas
Keyword(s):  
Nitrogenase Activity ◽  
Rhodopseudomonas Capsulata

scholarly journals Regulation of nitrogenase A and R concentrations in Rhodopseudomonas capsulata by glutamine synthetase

1980 ◽  
Vol 187 (1) ◽  
pp. 273-276 ◽  
Author(s):  
D C Yoch
Keyword(s):  
Glutamine Synthetase ◽  
Nitrogenase Activity ◽  
Photosynthetic Bacterium ◽  
Rhodopseudomonas Capsulata ◽  
Wild Type ◽  
Sulphur Bacteria ◽  
Intracellular Concentrations ◽  
Methionine Sulphoximine ◽  
Negative Mutant

Nitrogen-starved purple non-sulphur bacteria have an active unregulated form of nitrogenase (nitrogenase A); however, the nitrogenase of a glutamine synthetase-negative mutant of Rhodopseudomonas capsulata, when nitrogen-starved, was predominantly inactive and required activation by Mn2+ and activating-factor protein. This regulatory form of nitrogenase has been called nitrogenase R. Treatment of wild-type cells (containing nitrogenase A) with methionine sulphoximine, an inhibitor of glutamine synthetase, converted the enzyme into nitrogenase R. Glutamine synthetase thus appears to control the intracellular concentrations of nitrogenase A and R and in this way regulates nitrogenase activity in the photosynthetic bacterium.

Regulation of Nitrogenase Activity in Rhodopseudomonas capsulata AD 2

1983 ◽  
Vol 38 (5-6) ◽  
pp. 436-438
Author(s):  
Kassem Alef
Keyword(s):  
Nitrogenase Activity ◽  
Rhodopseudomonas Capsulata ◽  
N Starvation

Regulation of nitrogenase activity in Rhodopseudomonas capsulata AD 2 differs in several respects from other Rhodospirillaceae: 1)Nitrogenase activity in this strain grown under severe N-starvation was fully derepressed, but in contrast to other Rhodospirillaceae, it was inhibited by ammonia in vivo. 2)Nitrogenase in extracts of glutamate grown cells was fully active and not further stimulated by Mn2+. 3)Treatment of N-starved or glutamate grown cells with ammonia before harvest did not cause inactivation of nitrogenase in vitro.

scholarly journals Screening, Identification and Growth-Promotion Products of Multifunctional Bacteria in a Chinese Fir Plantation

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 120
Author(s):  
Guangyu Zhao ◽  
Yihui Wei ◽  
Jiaqi Chen ◽  
Yuhong Dong ◽  
Lingyu Hou ◽  
...  
Keyword(s):  
High Efficiency ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Acc Deaminase ◽  
Inorganic Phosphorus ◽  
Chinese Fir ◽  
Biochemical Tests ◽  
Growth Promoting ◽  
Chinese Fir Plantation ◽  
Physiological And Biochemical

Purpose: This research was aimed to screen and identify multifunctional phosphorus-dissolving bacteria of a Chinese fir (Cunninghamia lanceolata) plantation and study its phosphorus-dissolving characteristics in order to provide strain resources and a theoretical basis for developing the appropriate bacterial fertilizer of a Chinese fir plantation. Methods: First, phosphorus-dissolving bacteria were isolated from the woodland soil of a Chinese fir plantation by Pikovskava inorganic phosphorus medium (PVK). Then, some growth-promoting indicators of primary screening strains were determined, including the capacity of phosphorus-solubilized, nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, production of indole-3-acetic acid (IAA), secretion of iron carrier and so on. Finally, the screening multifunctional phosphorus-dissolving bacteria were identified, which were combined with colony characteristics, physiological and biochemical tests and molecular biotechnology. Results: (1) Thirteen phosphorus-dissolving bacteria were isolated and screened in total, and P5 (195.61 mg·L−1) had the strongest capacity of phosphorus-solubilized. Five phosphorus-dissolving bacteria were provided with nitrogenase activity, and the highest activity of nitrogenase was P10 and P5 (71.90 C2H4 nmol·mL−1·h−1 and 71.00 C2H4 nmol·mL−1·h−1, respectively). Four strains were provided with ACC deaminase activity, and the highest activity of ACC deaminase was P5 and P9, (0.74 μmol·mg−1·h−1 and 0.54 μmol·mg−1·h−1, respectively). Most strains could secrete IAA, and three strains of bacteria had a strong secretory ability, which could secrete IAA with a concentration greater than 15 mg·mL−1, and P5 was 18.00, P2 was 17.30, P6 was 15.59 (mg·mL−1). P5 produced carriers of iron better than others, and the ratio of the diameter of the iron production carrier ring to the diameter of the colony was 1.80, respectively, which was significantly higher than other strains. Combining all kinds of factors, P5 multifunctional phosphorus-dissolving bacteria were screened for eventual further study. (2) Strain P5 was identified as Burkholderia ubonensis, based on the colony characteristics, physiological and biochemical tests, 16SrDNA sequence analysis and phylogenetic tree construction. Conclusion: P5 has a variety of high-efficiency growth-promoting capabilities, and the ability to produce IAA, ACC deaminase activity and siderophore performance are significantly higher than other strains, which had great potential in the development of microbial fertilizer.

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