Drug resistance in the fission yeast Schizosaccharomyces pombe: pleiotropic mutations affecting the oleic acid and sterol composition of cell membranes

1984 ◽  
Vol 8 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Paul A. Johnston ◽  
Alan Coddington
Keyword(s):  
Drug Resistance ◽  
Oleic Acid ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Cell Membranes ◽  
Sterol Composition

scholarly journals Loss of Regulators of Vacuolar ATPase Function and Ceramide Synthesis Results in Multidrug Sensitivity in Schizosaccharomyces pombe

2008 ◽  
Vol 7 (6) ◽  
pp. 926-937 ◽  
Author(s):  
Keren Dawson ◽  
W. Mark Toone ◽  
Nic Jones ◽  
Caroline R. M. Wilkinson
Keyword(s):  
Drug Resistance ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Pathogenic Fungi ◽  
Vacuolar Atpase ◽  
Ceramide Synthase ◽  
Fluorescent Properties ◽  
Ceramide Synthesis ◽  
Increased Sensitivity ◽  
In Cells

ABSTRACT We undertook a screen to isolate determinants of drug resistance in fission yeast and identified two genes that, when mutated, result in sensitivity to a range of structurally unrelated compounds, some of them commonly used in the clinic. One gene, rav1, encodes the homologue of a budding yeast protein which regulates the assembly of the vacuolar ATPase. The second gene, lac1, encodes a homologue of genes that are required for ceramide synthesis. Both mutants are sensitive to the chemotherapeutic agent doxorubicin, and using the naturally fluorescent properties of this compound, we found that both rav1 and lac1 mutations result in an increased accumulation of the drug in cells. The multidrug-sensitive phenotype of rav1 mutants can be rescued by up-regulation of the lag1 gene which encodes a homologue of lac1, whereas overexpression of either lac1 or lag1 confers multidrug resistance on wild-type cells. These data suggest that changing the amount of ceramide synthase activity in cells can influence innate drug resistance. The function of Rav1 appears to be conserved, as we show that SpRav1 is part of a RAVE-like complex in fission yeast and that loss of rav1 results in defects in vacuolar (H+)-ATPase activity. Thus, we conclude that loss of normal V-ATPase function results in an increased sensitivity of Schizosaccharomyces pombe cells to drugs. The rav1 and lac1 genes are conserved in both higher eukaryotes and various pathogenic fungi. Thus, our data could provide the basis for strategies to sensitize tumor cells or drug-resistant pathogenic fungi to drugs.

scholarly journals Upstream – News in Genomics

2002 ◽  
Vol 3 (3) ◽  
pp. 221-225
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ovarian Cancer ◽  
Oryza Sativa ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Proteomic Analysis ◽  
Large Scale ◽  
Protein Complexes ◽  
The Other ◽  
Blood Samples

In recent months a bumper crop of genomes has been completed, including the fission yeast (Schizosaccharomyces pombe) and rice (Oryza sativa). Two large-scale studies ofSaccharomyces cerevisiaeprotein complexes provided a picture of the eukaryotic proteome as a network of complexes. Amongst the other stories of interest was a demonstration that proteomic analysis of blood samples can be used to detect ovarian cancer, perhaps even as early as stage I.

scholarly journals Analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.

1986 ◽  
Vol 83 (21) ◽  
pp. 8253-8257 ◽  
Author(s):  
L. Clarke ◽  
H. Amstutz ◽  
B. Fishel ◽  
J. Carbon
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Centromeric Dna

Transport of l-lysine in the fission yeast Schizosaccharomyces pombe

1989 ◽  
Vol 978 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Hana Sychrová ◽  
Jaroslav Horák ◽  
Arnošt Kotyk
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast
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