Lectin-gene expression in pea (Pisum sativum L.) roots

Planta ◽  
1988 ◽  
Vol 173 (3) ◽  
pp. 367-372 ◽  
Author(s):  
Dominique Buffard ◽  
Pierre-Alexandre Kaminski ◽  
A. Donny Strosberg
Keyword(s):  
Gene Expression ◽  
Pisum Sativum ◽  
Lectin Gene ◽  
Pisum Sativum L

scholarly journals Transcriptome Analysis of Alternative Splicing Events Induced by Arbuscular Mycorrhizal Fungi (Rhizophagus irregularis) in Pea (Pisum sativum L.) Roots

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1700
Author(s):  
Evgeny A. Zorin ◽  
Alexey M. Afonin ◽  
Olga A. Kulaeva ◽  
Emma S. Gribchenko ◽  
Oksana Y. Shtark ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alternative Splicing ◽  
Pisum Sativum ◽  
Mycorrhizal Fungi ◽  
Intron Retention ◽  
Protein A ◽  
Fine Tuning ◽  
Mrna Isoforms ◽  
Pisum Sativum L ◽  
Mycorrhizal Roots

Alternative splicing (AS), a process that enables formation of different mRNA isoforms due to alternative ways of pre-mRNA processing, is one of the mechanisms for fine-tuning gene expression. Currently, the role of AS in symbioses formed by plants with soil microorganisms is not fully understood. In this work, a comprehensive analysis of the transcriptome of garden pea (Pisum sativum L.) roots in symbiosis with arbuscular mycorrhiza was performed using RNAseq and following bioinformatic analysis. AS profiles of mycorrhizal and control roots were highly similar, intron retention accounting for a large proportion of the observed AS types (67%). Using three different tools (SUPPA2, DRIMSeq and IsoformSwitchAnalyzeR), eight genes with AS events specific for mycorrhizal roots of pea were identified, among which four were annotated as encoding an apoptosis inhibitor protein, a serine/threonine-protein kinase, a dehydrodolichyl diphosphate synthase, and a pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1. In pea mycorrhizal roots, the isoforms of these four genes with preliminary stop codons leading to a truncated ORFs were up-regulated. Interestingly, two of these four genes demonstrating mycorrhiza-specific AS are related to the process of splicing, thus forming parts of the feedback loops involved in fine-tuning of gene expression during mycorrhization.

Research Note: Boron deficiency affects early infection events in the pea-Rhizobium symbiotic interaction

2001 ◽  
Vol 28 (8) ◽  
pp. 819 ◽  
Author(s):  
Miguel Redondo-Nieto ◽  
Rafael Rivilla ◽  
Abdelaziz El-Hamdaoui ◽  
Ildefonso Bonilla ◽  
Luis Bolaños
Keyword(s):  
Gene Expression ◽  
Pisum Sativum ◽  
Cell Invasion ◽  
Root Hair ◽  
Boron Deficiency ◽  
Symbiotic Interaction ◽  
Pisum Sativum L ◽  
Plant Exudates ◽  
Hair Curling ◽  
Root Hair Curling

The effects of the deprivation of boron (B) on Rhizobium–legume signaling and preinfection events have been investigated in pea (Pisum sativum L. cv. Argona). The capacity of root exudates to induce the activity of nodulation genes was modulated by B nutrition in the host plant. Exudates derived from B-deficient pea plants led to a low level of nod-gene expression that could be correlated with poor root hair curling. However, inoculation of B-deficient plants with bacteria grown in the presence of the nod-gene inducing hesperetin, restored root hair curling. The attachment of bacteria to roots was also diminished in plants grown in the absence of the micronutrient, and it was not recovered by hesperetin. Both phenomena provoked a reduction in nodulation of more than 50%. Furthermore, infection thread development was arrested at very early stages, and cell invasion by endocytosis was precluded, leading to almost empty of bacteria B-deficient nodules.

scholarly journals Regulation of storage-protein synthesis in pea (Pisum sativum L.) cotyledons under conditions of sulphur deficiency

1985 ◽  
Vol 232 (1) ◽  
pp. 261-265 ◽  
Author(s):  
I M Evans ◽  
J A Gatehouse ◽  
D Boulter
Keyword(s):  
Gene Expression ◽  
Pisum Sativum ◽  
Gene Transcription ◽  
Storage Protein ◽  
Control Of Gene Expression ◽  
Sulphur Deficiency ◽  
Pisum Sativum L ◽  
S Deficiency ◽  
Vicilin Gene ◽  
Storage Protein Genes

The effects of sulphur deficiency on the expression of storage-protein genes in developing pea (Pisum sativum) cotyledons were studied. Legumin-gene transcription was decreased by S-deficiency, but not to the same extent as the decrease in the level of legumin mRNA. Vicilin-gene transcription was not significantly affected. Control of gene expression may thus occur during transcription and/or post-transcriptional events.

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